FastQCFastQC Report
Wed 25 May 2016
SRR1294830_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294830_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3910506
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC95110.24321660675114676No Hit
CCCATGTACTCTGCGTTGATACCAC84990.21733760285753304No Hit
GAGTACATGGGAAGCAGTGGTATCA43510.11126437345959832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG802.8768109E-511.871839
TAGACGT600.00587602611.0839234
GCGACGT803.782747E-410.6846488
CGTCCCT1002.407327E-510.44747810
CGACGTA1054.1206014E-59.9497249
GTGTTAG5050.09.7820721
TCTATAC4250.09.6112923
ATCGACC1201.7073167E-59.4974648
TGCCGAC1201.7083525E-59.49697718
GTCTAGA4550.09.1867141
AACCGTG1252.765035E-59.1175657
GTTCTAT3350.09.0745291
CGACCGC1054.5061787E-49.04543510
GTATTAG5150.09.0387381
CTATACT6550.08.9928464
ACGTCGT850.00743648978.9415385
CATGCGG850.00745370058.93901810
CCTATAC3950.08.8982953
CCAATAC3850.08.8826793
GTTCTAG3750.08.866571
Produced by FastQC (version 0.11.5)