FastQCFastQC Report
Wed 25 May 2016
SRR1294830_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294830_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3910506
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT116150.29702038559715804No Hit
GTACATGGGAAGCAGTGGTATCAAC95600.24446964152465178No Hit
CCCATGTACTCTGCGTTGATACCAC89250.2282313337455562No Hit
GGTATCAACGCAGAGTACTTTTTTT72880.1863697434551948No Hit
TATCAACGCAGAGTACTTTTTTTTT66930.17115432120549107No Hit
GAGTACATGGGAAGCAGTGGTATCA45130.11540705985363532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCTT851.736953E-814.51802619
TTAGACG751.43390025E-512.705664
CGTATCC907.2173116E-611.6468543
GCGTATA803.659927E-410.7228781
TTGACGC1201.5482146E-610.27978917
GTCTTAG4200.09.5314471
TGCGCTG3200.09.5292445
TACGACA1002.673415E-49.5292444
GTACGTC800.00440490949.5292443
TACGTCT900.00108341559.5292444
TTGCGGA1502.681918E-79.49255719
TAGACAG5200.09.345995
GTCCTAG4700.09.328651
ACCGGAG1856.843038E-99.23257518
GTCACCG1752.7241185E-89.2179215
TCACCGG1651.08613676E-79.20148916
TGGACCG1451.6382473E-69.200655
GACACCG1054.3592966E-49.0754725
AAGGGCG1054.3592966E-49.0754725
CGACGTA850.00751132758.9306299
Produced by FastQC (version 0.11.5)