FastQCFastQC Report
Wed 25 May 2016
SRR1294829_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294829_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2668966
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC88760.3325632473399811No Hit
CCCATGTACTCTGCGTTGATACCAC80840.3028888341027948No Hit
GAGTACATGGGAAGCAGTGGTATCA39430.14773511539674916No Hit
CATGTACTCTGCGTTGATACCACTG30010.11244054813736855No Hit
ATTCCATTCCATTCCATTCCATTCC28460.10663305564776772No Hit
GCGTTGATACCACTGCTTCCCATGT28140.10543408945636625No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG602.570577E-514.2494352
CACCGCT853.953848E-612.2920167
AGTACGG1501.4551915E-1112.0330843
GTACGGT951.04024E-612.0004284
TACGGTC600.005876790311.0835195
AACGCTG700.001491798610.8573265
TAGACTG3400.010.61782555
TTAGACA3400.010.0591814
TCGTGGG1803.965397E-1010.02493412
TTAGAAC2500.09.8797953
TACGGGC1356.4457345E-79.8520175
TACGGTG900.00111389139.500165
GTCTTAT2700.09.4998031
GTCTAGG3250.09.3536521
GTCCTAT2357.2759576E-129.2976791
GTTAGAC1356.852506E-69.1479583
GTACGGG1151.11064925E-49.0872794
ACACCGC1054.510272E-49.04437356
CTACGGT850.00743549468.9414954
CTAGGAC3350.08.7908623
Produced by FastQC (version 0.11.5)