FastQCFastQC Report
Wed 25 May 2016
SRR1294829_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294829_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2668966
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC90590.33941983524705827No Hit
CCCATGTACTCTGCGTTGATACCAC83120.3114314682165303No Hit
GTATCAACGCAGAGTACTTTTTTTT73060.27373896857434676No Hit
GGTATCAACGCAGAGTACTTTTTTT46280.17340048543143674No Hit
TATCAACGCAGAGTACTTTTTTTTT44300.16598188212214018No Hit
GAGTACATGGGAAGCAGTGGTATCA42440.15901289113461917No Hit
CATGTACTCTGCGTTGATACCACTG31710.11881005602918883No Hit
GCGTTGATACCACTGCTTCCCATGT29560.11075450193071025No Hit
ATTCCATTCCATTCCATTCCATTCC27420.10273641552571297No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG603.9812364E-412.7085715
CGCAATC604.1374742E-412.65071717
TATACCG802.7728287E-511.9142865
GCACCGC855.3741744E-511.1665826
GTCTTAG2053.6379788E-1210.2323171
TAGAGCG1201.6360515E-59.5314285
GACCGTG1002.7900218E-49.4885717
AGTGCGG800.0045651359.48786110
GTCTAGA1951.5770638E-99.2901811
GTCTAAT2700.09.1815231
TAGTACC1356.569593E-69.1784144
GGACCGT950.0018391018.9920386
ACCGTCT950.00184569678.98858
CTAGAAC2451.6370905E-118.9478723
GTCTTAT2159.731593E-108.86945151
TATTCCG1401.0412086E-58.8506135
TAGCACT2950.08.7236814
GTCTATA2850.08.6982861
AGCACCG1106.9155154E-48.6649365
CTTATAC3300.08.6649363
Produced by FastQC (version 0.11.5)