FastQCFastQC Report
Wed 25 May 2016
SRR1294828_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294828_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4307532
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44120.10242524025358371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC1052.0019797E-812.6644488
GAACCGT604.1103622E-412.66091256
GGTATCA17050.012.2033331
AGTGCGC550.003073990312.0887928
ATCGCTC951.364279E-510.9980738
CGCGTGC700.001494511810.8552428
AACCGTG1053.4780314E-610.8551167
GTATCAA37350.010.1494151
GACGCGT750.002659200710.1287316
ACCGGAC856.5952033E-410.0592863
AGGACCG1054.103572E-59.9535335
GTATTAG6750.09.5711431
AATACCG900.00111317679.50115
CTAGGAC4100.09.5004363
TTGCGTG1451.7209932E-69.16739516
TCTATAC4500.09.0781953
CTAGACT3050.08.7225254
CTCGTTG1201.7753814E-48.70366917
CTTGCGT1651.0593212E-68.63173815
GGGCCGA1000.00291853898.5461176
Produced by FastQC (version 0.11.5)