FastQCFastQC Report
Wed 25 May 2016
SRR1294828_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294828_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4307532
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT148290.34425745415240094No Hit
GGTATCAACGCAGAGTACTTTTTTT90850.21090963456568634No Hit
TATCAACGCAGAGTACTTTTTTTTT86950.20185572620238226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22950.012.9610351
ACGCAAC550.003089423512.0807116
TCGGCAC600.00573641511.1198283
TACGACC600.00573641511.1198284
GTATCAA50800.010.8100211
CGTATAC803.660682E-410.7226913
AAAACCG750.002577923710.1666995
CGTCCGC900.00112764969.48768910
TCGACCG1054.5541956E-49.0358959
TATTCCG1752.49991E-78.71431355
GTATTAA9300.08.7137231
CACGGGT1000.0028213368.5781534
GTCCTAG4250.08.5243721
GTATAGA7050.08.3843681
TACTCCG1606.177099E-68.339875
AGGACCG1502.483736E-58.2604445
CTACACT5450.08.2196384
GTCCTAT3150.08.1718621
TATCAAC66100.08.148152
TAGACAG5150.08.143235
Produced by FastQC (version 0.11.5)