FastQCFastQC Report
Wed 25 May 2016
SRR1294827_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294827_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4490811
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC148110.32980679881651664No Hit
CCCATGTACTCTGCGTTGATACCAC138420.3082294044438744No Hit
GAGTACATGGGAAGCAGTGGTATCA55660.12394197840879967No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTA751.4890989E-512.659162514
GCGGGAC2000.011.8756493
TCTCACG7150.011.61048919
CGTCGTA1001.9365016E-611.3978249
TGCGCTG4500.010.1342265
AGCGCAC750.002653679810.13128610
CGAATTA856.632107E-410.05320115
GCGCTGA3800.09.9953036
GTCCTAA4600.09.9138071
CTAATAC4350.09.609723
TCGTCCG1106.8567184E-59.4981879
CGGACGT900.00111643649.49808110
ACCGTAC800.00452578779.498088
AGACCGT2106.730261E-119.4955376
CGTCTCG1201.7140874E-59.49437214
CTGCGCT4850.09.4032054
TTAGACA4200.09.2749384
GTATTAA7600.09.2507121
TCTATAC4650.09.1940513
TACGCAG1151.1104641E-49.0877575
Produced by FastQC (version 0.11.5)