FastQCFastQC Report
Wed 25 May 2016
SRR1294827_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294827_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4490811
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC145370.3237054509753361No Hit
CCCATGTACTCTGCGTTGATACCAC143080.31860614931245157No Hit
GAGTACATGGGAAGCAGTGGTATCA55590.1237861045588425No Hit
GTATCAACGCAGAGTACTTTTTTTT54900.12224963375212182No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCT550.002989510512.1338714
TCGTCCG1158.9851346E-710.7244929
CTAGACT2700.010.5930624
ACCGTCC909.655928E-510.5411688
TAGCCCG1002.30896E-510.4871325
CGTCGTA1002.4375535E-510.4357569
GTATTAG3750.010.4269561
ACCGATC750.002679380610.1195218
TGCGCTG4650.010.0463245
TACGGGT951.5875365E-410.0355334
CGGGACT2500.09.9151074
GCGCTGA4600.09.9037226
TCTAGAC3250.09.6804293
TAGACAG5800.09.5337565
TCTCACG6750.09.42139219
GTATTAA5600.09.36654951
TGGACCG1856.313712E-99.2760885
TAGGTTG3450.09.1192465
ATGCGGG2002.4811015E-99.0600081
GCTGCGC4850.09.0423263
Produced by FastQC (version 0.11.5)