FastQCFastQC Report
Wed 25 May 2016
SRR1294826_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294826_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2640838
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC112220.42494087104169204No Hit
CCCATGTACTCTGCGTTGATACCAC110300.4176704515763557No Hit
GAGTACATGGGAAGCAGTGGTATCA41710.15794228953082318No Hit
CATGTACTCTGCGTTGATACCACTG36800.1393497064189473No Hit
GCGTTGATACCACTGCTTCCCATGT32240.12208246018877342No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCCG456.77669E-414.774289
AGTACCG1051.9912477E-812.6687165
CGTCCGC951.0434123E-611.99738710
TCGACCG951.0436524E-611.997169
ATCGACC1052.7308124E-711.759128
GTCGTAC855.31403E-511.1789161
GGGTACG600.00586793211.0857591
TCGTCTA951.3704059E-510.99322314
TAGACAG3750.010.8950975
ATCGTCC803.7808117E-410.684978
CGCACGA803.782138E-410.68456512
CGGGACT2050.010.6606664
ACACCGT1252.2586937E-710.6342536
ACGCGCG909.581422E-510.54885316
CTCGCAT750.002654658610.1305513
AACGTGT750.002660514810.127866
CGATTTC750.00266260910.12689916
GAACCGT856.6290365E-410.0533896
TCGCATC951.6550666E-49.99383914
CTTAGAC1554.0563464E-89.808043
Produced by FastQC (version 0.11.5)