FastQCFastQC Report
Wed 25 May 2016
SRR1294826_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294826_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2640838
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC112030.4242214024487681No Hit
GTACATGGGAAGCAGTGGTATCAAC111440.42198726313389917No Hit
GAGTACATGGGAAGCAGTGGTATCA42230.15991136146935178No Hit
CATGTACTCTGCGTTGATACCACTG39580.14987666793646562No Hit
GCGTTGATACCACTGCTTCCCATGT31950.12098432391536323No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG400.00532744814.2309568
ACACGAC551.9747538E-413.8067736
ACGTCGC551.9747538E-413.8067736
GCGGCTT500.001514789813.28272913
GTATTAA2050.013.0231211
GTCCTAA1850.012.8848441
GGGTCGT604.122327E-412.656216
AGACCGA1155.3969416E-912.3810756
CGTACCC701.0574686E-412.2557443
CGCACGA701.1047752E-412.19842512
TTACGCT657.8016974E-411.7319944
GTCGTAC907.168277E-611.65362551
CGTCCGC1406.9667294E-1011.52029910
TTTCCCG1001.951681E-611.38994219
TCGTCCG1201.3002864E-711.06852159
TTAGACT1451.3704266E-810.518344
TACATCG1201.4598681E-610.3265985
AAACCGA750.002666826110.1249686
AATCGGA750.002676910.12036617
ACCGTTC750.002678161510.1197928
Produced by FastQC (version 0.11.5)