FastQCFastQC Report
Wed 25 May 2016
SRR1294825_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294825_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4022641
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG1251.8402716E-811.3977359
CGTAGCA1106.036438E-610.3658392
AAGCGTC1201.5342775E-610.28705918
GGTATCA19300.010.0435841
CGCCTAA1252.593326E-69.87385514
TGCGCTG3600.09.7668175
CGGACTG1201.6959384E-59.5028495
GTCTTAG4100.09.502021
GCTGCGC3300.09.5020193
ACTGCGT2201.6370905E-119.4981128
CGACCGC1701.6734703E-89.49787510
CTGCGCT3900.09.2595324
GTATCAA35000.09.2576821
CGTCCGC2002.6702764E-99.02298210
AGTACCG2151.036824E-98.8398595
CCTAGAC3450.08.8134673
GCGCTGA4100.08.8011546
GTCCTAA2607.2759576E-128.7710951
TAAACAG6950.08.7508245
TCGTCCG1752.636407E-78.6839899
Produced by FastQC (version 0.11.5)