FastQCFastQC Report
Wed 25 May 2016
SRR1294825_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294825_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4022641
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85790.21326785064836756No Hit
GGTATCAACGCAGAGTACTTTTTTT51580.12822421886516844No Hit
TATCAACGCAGAGTACTTTTTTTTT50020.12434616959355806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG1052.7683564E-711.7472089
GGTATCA22550.010.5687791
TCGTCTA1502.4967449E-810.12067214
ACTGTCG750.002676751510.1206728
AGACCGT1901.0186341E-109.9925976
TCGTCCG1054.165442E-59.9399459
ACGGGTC1252.470164E-69.9115135
ATCGACC1252.614228E-69.8676548
GTATCAA45750.09.6476441
AAGCGTC1201.7270873E-59.48824718
GACGCGT800.00456491629.488139
TACATCG2053.9835868E-109.2978545
AGGGCCG1451.6358299E-69.2016715
TAGCCCG1252.654843E-59.149095
TAAGGCG1151.0735596E-49.1159415
CCGTGAC1252.7973325E-59.10860359
GTCTAGT2750.09.0130561
TACGGGT850.0072472198.9696954
CTAGACT4050.08.9420114
TAGACAG6100.08.9053635
Produced by FastQC (version 0.11.5)