FastQCFastQC Report
Wed 25 May 2016
SRR1294824_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294824_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2699963
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC68360.2531886548074918No Hit
CCCATGTACTCTGCGTTGATACCAC62050.22981796417210162No Hit
GAGTACATGGGAAGCAGTGGTATCA30350.11240894782632208No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAC400.00527909814.2505443
ATCGCTA400.00528495214.24816210
CGGCGTA604.0994977E-412.66456212
ACGACAG701.0901399E-412.2158865
CTATGCG600.005883211.0819049
TAGACAG3700.011.0419865
GCACCGT1408.416464E-910.852526
GTCGTAC750.002646996410.1340961
GATACGA951.6438989E-410.00056652
GTCTAGC1356.440714E-79.8525921
GTATTAA5300.09.6799741
GTGCTCG900.00111330539.5007141
ATCGGCG800.0045220719.49877510
TTGGCGT1054.4882938E-49.0488034
TAACGTG1054.4882938E-49.0488035
GGATACG950.00182311389.0006771
ATTAGAC1901.0579242E-89.0003423
TACGTTC950.00183268248.99550117
GGACCGA850.00746373938.9373696
ATTGGAC1606.740793E-78.90658953
Produced by FastQC (version 0.11.5)