FastQCFastQC Report
Wed 25 May 2016
SRR1294824_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294824_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2699963
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC68130.2523367912819546No Hit
CCCATGTACTCTGCGTTGATACCAC64710.23966995103266228No Hit
GTATCAACGCAGAGTACTTTTTTTT64290.23811437415994222No Hit
GGTATCAACGCAGAGTACTTTTTTT38420.14229824630930127No Hit
TATCAACGCAGAGTACTTTTTTTTT37000.13703891497772377No Hit
GAGTACATGGGAAGCAGTGGTATCA30650.11352007416397929No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGA603.9755745E-412.7107261
ACGAAAC802.9032422E-511.86091315
TAGGACG657.8182475E-411.729054
AAGGGCG907.209861E-611.6475975
TAGGACC1604.3655746E-1111.31674
TACGATG1002.4318118E-510.4376047
GTCGTAG750.002573434510.168581
ACAATCC2500.09.8679158
TAGACTG3700.09.5298535
GTCACCG1002.7895786E-49.4887315
TTAGGAC2950.09.368333
GTCCTAG2600.09.1663891
CTAGACT2304.0017767E-119.1155114
CTTTGCG1151.1255502E-49.07617717
CGGGACA950.00177300949.0282824
GTTCTAA2356.184564E-118.9245521
GTCCAGG8100.08.8268931
CTGTGCG1304.4337987E-58.7585059
GTCTTAG2409.458745E-118.7386241
TGGCGTA1201.7054229E-48.7356995
Produced by FastQC (version 0.11.5)