FastQCFastQC Report
Wed 25 May 2016
SRR1294823_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294823_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3998105
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC78590.1965681241488155No Hit
CCCATGTACTCTGCGTTGATACCAC74030.18516272083899749No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA500.001503357413.29636811
TGCACCG1459.640644E-1111.7955015
TCGTGGG3000.011.39688712
TCGTCGA752.0806397E-411.39688713
GTCTCGT1553.6834535E-910.416779
CCGGATA1303.835048E-710.2329784
CGAGAAA1554.105823E-89.80082514
CGCGTCC800.0045273249.49764310
TTATACT6300.09.0495724
ATTCGTC950.00182930298.99754111
GTATACG850.00742585968.9431071
ATAACGC850.00742662378.9429943
CGTCGAG850.0074741218.93604614
CGGATAG1401.083295E-58.8232225
CCGATCT1107.1661785E-48.6342219
CGGTACT1000.00290079488.5518464
GTACACT4450.08.5411684
CTATACT6450.08.39716154
ACCGAAT1252.7524607E-48.3579268
TGGACCG1606.43794E-68.314195
Produced by FastQC (version 0.11.5)