FastQCFastQC Report
Wed 25 May 2016
SRR1294823_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294823_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3998105
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT90920.22740773441417872No Hit
GTACATGGGAAGCAGTGGTATCAAC80580.20154548217217907No Hit
CCCATGTACTCTGCGTTGATACCAC76300.19084041064454285No Hit
GGTATCAACGCAGAGTACTTTTTTT55120.13786531369236174No Hit
TATCAACGCAGAGTACTTTTTTTTT51560.12896109531890734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGAC3100.010.1468153
ACCGCCC1054.1683983E-59.93938
CTTAGAC3550.09.9346113
AGGACCG2251.8189894E-129.7436765
AATACCG1002.6660966E-49.53185755
ACACCGT1651.0798976E-79.2049626
TTAGACA3950.09.16988854
TAGACGT1252.6497255E-59.1505824
GTGCTAG3350.09.1079181
ATACCGC1151.1209E-49.0798956
TCGTCCG950.00184673158.988179
GTTCTAG3400.08.9739781
GTCCTAA4100.08.837181
GTATTGG3250.08.8014021
TACATCG1304.2036703E-58.7986375
TAGCCCG1252.6476415E-48.3880345
GTATAGG6150.08.3720661
TAAGACT3650.08.3566974
CGAATTA1252.7836027E-48.3492215
TTGCGGA1150.00111951048.25465719
Produced by FastQC (version 0.11.5)