FastQCFastQC Report
Wed 25 May 2016
SRR1294822_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294822_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3965150
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC196070.4944831847471092No Hit
CCCATGTACTCTGCGTTGATACCAC179530.45276975650353707No Hit
GAGTACATGGGAAGCAGTGGTATCA90330.22780979281994376No Hit
CATGTACTCTGCGTTGATACCACTG68300.1722507345245451No Hit
GCGTTGATACCACTGCTTCCCATGT64390.16238982131823512No Hit
GTATCAACGCAGAGTACATGGGAAG55330.13954074877369077No Hit
ACGCAGAGTACATGGGAAGCAGTGG47080.11873447410564544No Hit
TATCAACGCAGAGTACATGGGAAGC42920.10824306772757651No Hit
ACTCTGCGTTGATACCACTGCTTCC40940.10324956180724564No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC551.9648483E-413.8152858
TCGTCTA2650.012.53883314
TCGTCCG2000.011.3976119
CACGGGT1950.011.208594
TATACCG600.00586653811.0863365
ATCGCTC1252.2496715E-710.6377698
ACGGGTC1856.002665E-1110.2730535
TGCACCG3150.010.2567465
AACGTCG750.002558935410.17572419
TTGCGTG3000.010.126471516
CGTCGAG856.638179E-410.0521414
TGGTCGG1901.0004442E-109.99790410
TTCGCGG1054.138682E-59.94576814
GCACCGT3100.09.8011746
ATTAGAC1651.0202712E-89.7899123
CGTAGCA1752.568413E-99.77345852
CGGGACT3250.09.6491344
CTCGTCT3850.09.6206313
TAGACAG4500.09.5025745
GCTCGTC3750.09.37065812
Produced by FastQC (version 0.11.5)