FastQCFastQC Report
Wed 25 May 2016
SRR1294822_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294822_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3965150
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC196720.4961224669936825No Hit
CCCATGTACTCTGCGTTGATACCAC184860.4662118709254379No Hit
GAGTACATGGGAAGCAGTGGTATCA93920.23686367476640227No Hit
GTATCAACGCAGAGTACTTTTTTTT82340.20765923105052772No Hit
CATGTACTCTGCGTTGATACCACTG70370.1774712179867092No Hit
GCGTTGATACCACTGCTTCCCATGT66030.16652585652497381No Hit
GTATCAACGCAGAGTACATGGGAAG57030.14382810234165164No Hit
ACGCAGAGTACATGGGAAGCAGTGG50750.12799011386706682No Hit
GGTATCAACGCAGAGTACTTTTTTT50510.12738484042217824No Hit
TATCAACGCAGAGTACTTTTTTTTT46500.11727172994716467No Hit
TATCAACGCAGAGTACATGGGAAGC44670.11265651992988916No Hit
ACTCTGCGTTGATACCACTGCTTCC42870.10811696909322473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG1052.7729766E-711.7456849
TCGTCCG1302.6775524E-911.676189
TCGTTAC700.001509406310.84230613
ACGGGTC1502.0172592E-910.8045
ACGTCGC1106.113416E-610.3548286
GCTCGTT3950.010.32763911
TATACTG5800.010.1903855
TTCGTCC951.667081E-49.98696316
TCTCGTA1356.5069617E-79.8455919
GTATTAA4650.09.6383761
CGGGACT2600.09.5329414
TACGTCG1201.6336213E-59.5329415
TCGTCTA3100.09.48701714
CGTCGAG1606.786104E-89.48701714
CGCACGA1502.7047827E-79.48701712
ACCGTCT2850.09.3204618
GTCTTAG2053.947207E-109.3032591
TCGGACG1752.7343049E-89.2158439
CGTAGCA1451.6277008E-69.2050352
CTAGACT2304.0017767E-119.1184644
Produced by FastQC (version 0.11.5)