FastQCFastQC Report
Wed 25 May 2016
SRR1294821_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294821_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148663
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC58683.947182553829803No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA57643.877225671485171No Hit
CCCATGTACTCTGCGTTGATACCAC56463.7978515165172233No Hit
GAGTACATGGGAAGCAGTGGTATCA30702.0650733538271124No Hit
CATGTACTCTGCGTTGATACCACTG24551.6513860207314532No Hit
ACGCAGAGTACATGGGAAGCAGTGG21141.4220081661206891No Hit
GCGTTGATACCACTGCTTCCCATGT20981.4112455688368994No Hit
GGTATCAACGCAGAGTACATGGGAA19411.3056375829897149No Hit
ACTCTGCGTTGATACCACTGCTTCC18351.2343353759846096No Hit
TATCAACGCAGAGTACATGGGAAGC18211.2249181033612937No Hit
GTATCAACGCAGAGTACATGGGAAG16571.1146014812024512No Hit
GTGGTATCAACGCAGAGTACATGGG14971.006975508364556No Hit
GTACTCTGCGTTGATACCACTGCTT12720.8556264840612661No Hit
GCTTCCCATGTACTCTGCGTTGATA11950.803831484633029No Hit
GATACCACTGCTTCCCATGTACTCT10980.7385832386000551No Hit
ATACCACTGCTTCCCATGTACTCTG9520.6403745383854759No Hit
GGGAAGCAGTGGTATCAACGCAGAG9090.6114500581852916No Hit
CAGTGGTATCAACGCAGAGTACATG8980.6040507725526864No Hit
CATGGGAAGCAGTGGTATCAACGCA8970.6033781102224495No Hit
CTGCTTCCCATGTACTCTGCGTTGA8790.5912701882781862No Hit
GTATCAACGCAGAGTACTTTTTTTT8670.5831982403153441No Hit
GGTATCAACGCAGAGTACTTTTTTT8140.5475471368127913No Hit
GTTGATACCACTGCTTCCCATGTAC8080.5435111628313702No Hit
GCAGAGTACATGGGAAGCAGTGGTA7970.536111877198765No Hit
TACCACTGCTTCCCATGTACTCTGC7810.5253492799149755No Hit
GCAGTGGTATCAACGCAGAGTACAT7620.5125686956404754No Hit
CTTCCCATGTACTCTGCGTTGATAC7490.5038240853473964No Hit
CCACTGCTTCCCATGTACTCTGCGT7210.4849895401007648No Hit
ACATGGGAAGCAGTGGTATCAACGC6860.4614463585424753No Hit
AAGCAGTGGTATCAACGCAGAGTAC6180.4157053200863698No Hit
TATCAACGCAGAGTACTTTTTTTTT5990.40292473581186977No Hit
ATGGGAAGCAGTGGTATCAACGCAG5570.3746729179419223No Hit
CCATGTACTCTGCGTTGATACCACT5430.3652556453186065No Hit
AAACAAAAAAAAAAAAAAAAAAAAA5300.35651103502552756No Hit
TTCCCATGTACTCTGCGTTGATACC5070.3410398014300801No Hit
TGATACCACTGCTTCCCATGTACTC5010.3370038274486591No Hit
ATCAACGCAGAGTACATGGGAAGCA4290.2885721396716062No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA3830.2576296724807114No Hit
GGAAGCAGTGGTATCAACGCAGAGT3520.2367771402433692No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3200.21525194567579023No Hit
TCAACGCAGAGTACATGGGAAGCAG2840.1910361017872638No Hit
GTACATGGTAAGCAGTGGTATCAAC2770.1863274654756059No Hit
AAAAACAAAAAAAAAAAAAAAAAAA2680.1802735045034743No Hit
CTCTGCGTTGATACCACTGCTTCCC2650.17825551751276378No Hit
TGGGAAGCAGTGGTATCAACGCAGA2490.16749292022897427No Hit
CTGCGTTGATACCACTGCTTCCCAT2480.1668202578987374No Hit
AAAAAGTACTCTGCGTTGATACCAC2200.14798571265210578No Hit
AGTGGTATCAACGCAGAGTACATGG2130.14327707634044787No Hit
ACACAAAAAAAAAAAAAAAAAAAAA2110.14193175167997418No Hit
ACCATGTACTCTGCGTTGATACCAC2030.13655045303807942No Hit
ACTGCTTCCCATGTACTCTGCGTTG1980.1331871413868952No Hit
AACGCAGAGTACATGGGAAGCAGTG1910.12847850507523728No Hit
ATGTACTCTGCGTTGATACCACTGC1890.1271331804147636No Hit
GTGGTATCAACGCAGAGTACTTTTT1840.12376986876357937No Hit
TCCATGTACTCTGCGTTGATACCAC1630.10964395982860564No Hit
CTGTACTTGTTCGCTATCGGTCTCT1600.10762597283789511No Hit
CCCCATGTACTCTGCGTTGATACCA1600.10762597283789511No Hit
AAAGTACTCTGCGTTGATACCACTG1590.10695331050765826No Hit
TCCCATGTACTCTGCGTTGATACCA1580.10628064817742142No Hit
TTGATACCACTGCTTCCCATGTACT1570.10560798584718459No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA1570.10560798584718459No Hit
GGTATCAACGCAGAGTACATGGGGG1560.10493532351694773No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA1510.1015720118657635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA903.5255653E-813.7300111
GAAAAAA3650.010.677291
ATGGTAA1002.711035E-49.5021915
CATGGTA1002.711035E-49.5021914
TGGTAAG1002.738729E-49.4925956
GGTAAGC1107.078324E-48.6325387
CACAAAA1855.8949627E-78.218112
ACATGGT1200.00166448677.9184923
TAAGCAG1200.00167559067.91315949
GTAAGCA1200.00167559067.91315948
ACACAAA1752.0663321E-57.60431431
AGTGGTA19750.07.2576314
CAGTGGT19700.07.23029813
AGCAGTG19900.07.109914311
AAGCAGT19950.07.044496510
GAAGCAG18150.06.95834839
TGGTATC20900.06.90370516
GTGGTAT21050.06.809415315
GGAAGCA18600.06.79000148
CCATGTA18250.06.71661662
Produced by FastQC (version 0.11.5)