##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294821_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	148663
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.211949173634327	33.0	31.0	34.0	18.0	34.0
2	30.385193356786825	33.0	31.0	34.0	23.0	34.0
3	30.77006383565514	34.0	31.0	34.0	25.0	34.0
4	33.52094334165192	37.0	35.0	37.0	23.0	37.0
5	34.34584261046797	37.0	35.0	37.0	30.0	37.0
6	34.16594579686943	37.0	35.0	37.0	27.0	37.0
7	34.384170910044865	37.0	35.0	37.0	29.0	37.0
8	34.34378426373745	37.0	35.0	37.0	28.0	37.0
9	35.67449869839839	39.0	37.0	39.0	27.0	39.0
10	35.90335860301487	39.0	37.0	39.0	28.0	39.0
11	35.77777254595966	39.0	37.0	39.0	27.0	39.0
12	35.970840087984236	39.0	37.0	39.0	28.0	39.0
13	35.60706429979215	39.0	37.0	39.0	27.0	39.0
14	36.99207603774981	41.0	38.0	41.0	27.0	41.0
15	36.907677095174996	41.0	38.0	41.0	27.0	41.0
16	36.839879458910424	40.0	38.0	41.0	27.0	41.0
17	36.87682207408703	40.0	38.0	41.0	27.0	41.0
18	36.802687958671626	40.0	38.0	41.0	27.0	41.0
19	36.78092060566516	40.0	37.0	41.0	27.0	41.0
20	36.80718806966091	40.0	38.0	41.0	27.0	41.0
21	36.74596234436275	40.0	37.0	41.0	27.0	41.0
22	36.796438925623725	41.0	37.0	41.0	27.0	41.0
23	36.800885223626594	40.0	37.0	41.0	26.0	41.0
24	36.94642917201994	41.0	37.0	41.0	27.0	41.0
25	36.04040009955403	40.0	35.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.33073182605401286
1101	2	-0.6467588458900622
1101	3	-0.7709949181456146
1101	4	-0.5139261060274407
1101	5	-1.3922628062227531
1101	6	-0.7562115914449805
1101	7	-0.934190048793937
1101	8	-0.7873629337901349
1101	9	-1.099918333716566
1101	10	-1.1123914091021305
1101	11	-0.5132862698037712
1101	12	-1.0232795977136675
1101	13	-0.28453408810297987
1101	14	-0.8577654648216111
1101	15	-1.3332770106257286
1101	16	-0.7225305678113543
1101	17	-0.9945783539856521
1101	18	-0.5775409681052466
1101	19	-0.977203598271501
1101	20	-0.47243038677682847
1101	21	-0.7036455911157873
1101	22	-0.22678865605394805
1101	23	-0.7597134552050946
1101	24	-0.5447203805398573
1101	25	-0.8519903641635338
1102	1	0.15919923345251874
1102	2	0.0032774385801843664
1102	3	0.32679172916933297
1102	4	0.22845415522074575
1102	5	0.18112253485126928
1102	6	0.20079131131264205
1102	7	0.15298339097384428
1102	8	0.11013343776284046
1102	9	0.5343342061963483
1102	10	0.15529727014315142
1102	11	0.17312519608882582
1102	12	-0.3348724859575043
1102	13	0.2197293371510156
1102	14	0.41636370789246513
1102	15	0.560137358024491
1102	16	0.07581848879242159
1102	17	0.3900370306297347
1102	18	0.2610838504724029
1102	19	0.28014400695346353
1102	20	0.08834610088645434
1102	21	-0.24818477834364927
1102	22	-0.4262625312353734
1102	23	0.13252166816210575
1102	24	-0.43396203511170484
1102	25	-0.42474072700824195
1103	1	-0.026773088617932927
1103	2	0.28499922147019063
1103	3	0.5226040943742234
1103	4	0.5551975053285787
1103	5	0.3267461869001522
1103	6	0.5006655025419704
1103	7	0.08886584963667588
1103	8	0.2998350912495411
1103	9	0.07549164688650478
1103	10	0.5145262421057666
1103	11	0.3750932047157107
1103	12	0.32674685264781544
1103	13	0.2764780790633097
1103	14	0.672564283018275
1103	15	0.706839370964822
1103	16	0.2469633486055045
1103	17	0.3278515525564032
1103	18	-0.024816580871956262
1103	19	0.18277700479674053
1103	20	0.3882202921157827
1103	21	0.0329528923968212
1103	22	0.14585285338001341
1103	23	-0.09752865534616006
1103	24	0.2729484609343089
1103	25	-0.199812617734338
1104	1	0.5582376949909822
1104	2	0.3590466693494143
1104	3	0.4998686522462563
1104	4	0.49768492445151225
1104	5	-0.2948390036102708
1104	6	-0.11170868868735795
1104	7	0.08086800635845748
1104	8	0.39378728391668716
1104	9	0.1977957446578884
1104	10	0.4437588086046915
1104	11	0.19716365762728572
1104	12	0.1507044371194226
1104	13	0.537037029458709
1104	14	0.40194063096939203
1104	15	0.7235988964860312
1104	16	0.2537031041770348
1104	17	0.1592677998605012
1104	18	0.7418530812416293
1104	19	0.5734132377226899
1104	20	0.26623071627106754
1104	21	0.49219983704096393
1104	22	0.4391220841492469
1104	23	0.38732936046979916
1104	24	0.14296104181137537
1104	25	0.24352850376099155
1105	1	0.2220010616691468
1105	2	0.44821534820419373
1105	3	0.1530680313424959
1105	4	0.26570838081578074
1105	5	0.573089592457336
1105	6	0.32605002121260895
1105	7	-0.02326272837765231
1105	8	0.04058359298878145
1105	9	-0.2167006334035122
1105	10	0.295151530232836
1105	11	-0.05982150967178512
1105	12	0.18515597213149704
1105	13	0.17201891976571204
1105	14	0.1520915450777025
1105	15	0.5511040706840262
1105	16	0.004349878926952044
1105	17	-0.27708956474798185
1105	18	0.01464111159692294
1105	19	0.0017074771155876078
1105	20	0.2831115372438191
1105	21	-0.26428946961650723
1105	22	0.06513536459077329
1105	23	0.3676027305974259
1105	24	0.30907435674755845
1105	25	-0.055518581440800574
1106	1	0.6048901166434035
1106	2	0.9633913986941458
1106	3	0.30613645851406446
1106	4	0.5350780868446776
1106	5	0.8102179764467081
1106	6	0.35463746515879535
1106	7	0.643902194392652
1106	8	0.5752331528625518
1106	9	0.22450514636729224
1106	10	0.5789083812542657
1106	11	0.8072063926700181
1106	12	0.49436540578039256
1106	13	0.6696937246153993
1106	14	-0.02451948299072626
1106	15	0.1073239677110962
1106	16	0.6336888591627954
1106	17	0.6906068311995313
1106	18	0.9991536510421994
1106	19	0.6916468559563071
1106	20	0.8162663288066838
1106	21	0.5938736262147515
1106	22	0.923630631157792
1106	23	0.8486897593301919
1106	24	0.79306787941821
1106	25	0.653250725983213
1107	1	-0.40971102295773676
1107	2	-0.6665943562916112
1107	3	-0.4926527152751099
1107	4	-0.32282789606130535
1107	5	-0.3514629352342027
1107	6	-0.8857471502258178
1107	7	-0.43302088253042825
1107	8	-0.34178963916023974
1107	9	-0.9207939989318561
1107	10	-0.1646813623354788
1107	11	-0.5635201030564758
1107	12	-0.37386821245322466
1107	13	-0.7134971585754784
1107	14	-0.1680380015092453
1107	15	0.019004879392014118
1107	16	-0.7473652718913399
1107	17	0.031062671655377017
1107	18	-0.8633819614934097
1107	19	-0.5686807793713271
1107	20	-0.6306709931306429
1107	21	-0.9602574279163036
1107	22	-0.4481642406370838
1107	23	-0.6495296138891788
1107	24	-0.6396244282740966
1107	25	-0.7954672227347359
1108	1	0.3609381859893439
1108	2	0.4662479786783855
1108	3	0.46324835764723815
1108	4	0.1908109473647741
1108	5	0.494890947289889
1108	6	0.38921193324389947
1108	7	-0.04593232097378319
1108	8	0.42367680928493456
1108	9	0.03703469719471286
1108	10	0.09120152546230287
1108	11	0.5539558016534727
1108	12	0.23167825054004254
1108	13	0.503342266774581
1108	14	0.6321984051101381
1108	15	0.6698141174352941
1108	16	0.4005525313456104
1108	17	0.5543578162271103
1108	18	0.2341812318144605
1108	19	0.6215719938601723
1108	20	0.3469794887751618
1108	21	0.02303044260560938
1108	22	0.3756400874225676
1108	23	0.013209065870775305
1108	24	-0.011601151314650338
1108	25	0.34804978035673884
1109	1	0.31013703022365036
1109	2	0.39086053449661406
1109	3	0.37455209637414555
1109	4	0.38230030906689905
1109	5	0.3812056307676954
1109	6	0.35926709478842156
1109	7	0.1459598075613755
1109	8	0.4687516713810709
1109	9	0.5516656401944502
1109	10	0.21882607672751675
1109	11	0.022741372093889822
1109	12	0.10650532347586505
1109	13	0.029776029142148275
1109	14	0.3443076775031031
1109	15	0.18139804178517238
1109	16	0.3672279538129999
1109	17	0.3058331686480926
1109	18	0.3890713465154505
1109	19	0.43290199998923384
1109	20	0.2612251765433058
1109	21	0.42103412004190943
1109	22	0.28577054885326447
1109	23	0.2710148622108548
1109	24	0.4067510069902269
1109	25	-0.2623871340990931
1110	1	-0.7411894735851234
1110	2	-0.9294967021726812
1110	3	-0.30696440831020766
1110	4	-0.9581252228479968
1110	5	-0.4285133080292596
1110	6	-0.538447641224181
1110	7	-0.7310387039525068
1110	8	-0.2997274460342112
1110	9	-0.4055184942946468
1110	10	-0.9683729426228069
1110	11	-0.4290229217507857
1110	12	-0.23215153669399768
1110	13	-0.44559390343818706
1110	14	-0.8060994672302826
1110	15	-1.091888010224281
1110	16	-0.7909368303565145
1110	17	-0.9647911199431007
1110	18	-0.361563449036602
1110	19	-0.5528960912462111
1110	20	-0.5125949793099451
1110	21	-0.2067567914369448
1110	22	-0.16904076364126297
1110	23	-0.18679093412922043
1110	24	-0.19032455556996553
1110	25	-0.12003532602624034
1111	1	-0.34329149870688624
1111	2	0.053362393785622686
1111	3	-0.06071985563140814
1111	4	-0.2683021005716526
1111	5	-0.08480656616819715
1111	6	-0.31453924166851266
1111	7	-0.37483943880681636
1111	8	-0.20817827060911043
1111	9	-0.2928977987464947
1111	10	-0.09244369371226924
1111	11	-0.0771644239297089
1111	12	-0.35558997128342185
1111	13	-0.16988681978442344
1111	14	-0.05625754635529745
1111	15	0.01540071916134167
1111	16	-0.18346865980195304
1111	17	-0.22751008089328195
1111	18	-0.2894451887614551
1111	19	0.14072093003038333
1111	20	0.14128864027477306
1111	21	0.3252435503557578
1111	22	0.4470576726571238
1111	23	0.4675541056041794
1111	24	0.6446408379188782
1111	25	0.5254060138011525
1112	1	0.23253561928890676
1112	2	0.11484642514917098
1112	3	0.025051802429405967
1112	4	-0.07404889728231012
1112	5	0.22423914046787274
1112	6	0.18598667150800452
1112	7	-0.13387558338512662
1112	8	0.23727018989958992
1112	9	0.002282924145070808
1112	10	-0.06394876160288021
1112	11	-0.04398408352045635
1112	12	-0.11410020268521492
1112	13	-0.5141687092470306
1112	14	-0.1442284776997198
1112	15	0.3956837560708877
1112	16	0.10062007609401036
1112	17	-0.010451369858671455
1112	18	-0.13444423255567983
1112	19	-0.1529298636204075
1112	20	0.049731937272291304
1112	21	0.2671540494951756
1112	22	0.4553766629038236
1112	23	0.5821553677958065
1112	24	0.28314724449757733
1112	25	0.4627745355070232
1113	1	0.1473985341518187
1113	2	0.10059678589953336
1113	3	-0.15135512397752038
1113	4	0.170179096945688
1113	5	0.28791157914031373
1113	6	-0.13778677726544686
1113	7	-0.24372406823361104
1113	8	-0.17687122424182178
1113	9	-0.23635343949129606
1113	10	0.02082757317345596
1113	11	-0.17637946591583642
1113	12	0.26667180308678695
1113	13	0.22104052596220214
1113	14	-0.332179415650657
1113	15	-0.1279453925582601
1113	16	-0.36546938999545375
1113	17	-0.1120025964098943
1113	18	-0.2693648674763196
1113	19	-0.11306694875749201
1113	20	0.06037407291442776
1113	21	0.0101194174605439
1113	22	-0.028389570862408675
1113	23	0.1319389175793546
1113	24	-0.08824525189491794
1113	25	-0.10306240532991495
1114	1	0.16248870713673114
1114	2	0.42179970578666115
1114	3	0.2372168303839075
1114	4	0.42683472202236317
1114	5	0.16518123930470807
1114	6	0.22761317366082068
1114	7	0.1400785326742522
1114	8	0.10153019889644099
1114	9	0.24182408473885886
1114	10	0.2390523308718997
1114	11	0.24169807058274984
1114	12	0.3667975545283255
1114	13	0.5028771104303686
1114	14	0.10614103582769019
1114	15	-0.05701851242085354
1114	16	0.13882456166691526
1114	17	0.07829613994147167
1114	18	0.2623490326586335
1114	19	0.3107614158603411
1114	20	0.29937848955042057
1114	21	0.1366836427089808
1114	22	0.11346418941240444
1114	23	0.3977038543969229
1114	24	0.5247930256170434
1114	25	0.2589637264330591
1115	1	0.06148053058675629
1115	2	-0.00942995206838404
1115	3	-0.12256724518963935
1115	4	-0.2529938085801575
1115	5	-0.13673191462083167
1115	6	-0.14879390739022114
1115	7	-0.153483426522385
1115	8	0.04870809839632528
1115	9	0.3336554731646757
1115	10	0.20701295641766393
1115	11	0.05116064103603435
1115	12	0.011903532142049755
1115	13	-0.21897729934973853
1115	14	-0.12424715183604462
1115	15	-0.10749084710371903
1115	16	0.01331848879242159
1115	17	0.1897353715045469
1115	18	0.14239983840603543
1115	19	0.07797583199116787
1115	20	0.019153039046329923
1115	21	-0.1581960905608497
1115	22	0.0948724612231473
1115	23	0.3175895414652743
1115	24	0.3811397748430494
1115	25	0.3427932096433466
1116	1	0.22328786221505936
1116	2	0.5035282747005851
1116	3	0.278882030172678
1116	4	0.36490900471907395
1116	5	0.019960327493407704
1116	6	-0.28775383918902975
1116	7	0.24583456154240935
1116	8	-0.20065251541441853
1116	9	0.542862634289996
1116	10	-0.2191592515959755
1116	11	-0.12189988752321312
1116	12	0.05998537357427125
1116	13	-0.16058002739747934
1116	14	-0.13856940247542582
1116	15	-0.24517200652400817
1116	16	-0.41276846772931464
1116	17	0.03150860253608556
1116	18	-0.07081414283863552
1116	19	-0.146235591708745
1116	20	0.07371399386304489
1116	21	-0.30499915961455315
1116	22	0.3719398099017539
1116	23	0.3984079557875191
1116	24	0.14149783110903513
1116	25	0.013637199413167878
1201	1	0.22526048701377377
1201	2	-0.1384604531577054
1201	3	0.26909942147702637
1201	4	0.2804484572150443
1201	5	-0.2916338754051466
1201	6	0.06760042812175726
1201	7	-0.004245953755500409
1201	8	0.07060352323292562
1201	9	-0.10882818696604346
1201	10	-0.05535087800499383
1201	11	-0.06779360732998185
1201	12	-0.3204494090344241
1201	13	0.29932335424503265
1201	14	0.007887924416685621
1201	15	0.1073239677110962
1201	16	-0.21816299268905937
1201	17	0.11653275712546218
1201	18	0.2815610584496113
1201	19	0.3305357448452
1201	20	0.21904410658446238
1201	21	-0.026496744155615204
1201	22	-0.08099899847184133
1201	23	0.13572679636722995
1201	24	0.06158639793672904
1201	25	0.38473220746469394
1202	1	-0.5407917802067175
1202	2	-0.6121970402120525
1202	3	-0.3699192701117582
1202	4	-0.7251763264471194
1202	5	-0.5470855888007833
1202	6	-0.611484030168306
1202	7	-0.17281639335397614
1202	8	-0.2982957498719543
1202	9	-0.4151625587209722
1202	10	-0.4621092719129223
1202	11	0.01060722340729825
1202	12	-0.533066231464332
1202	13	-0.47118547233856134
1202	14	-0.35080020296303616
1202	15	-0.06418866641836019
1202	16	0.12079512430644002
1202	17	-0.489905456789387
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2210	6	-0.37836253484120164
2210	7	0.08479108328153728
2210	8	0.2428257454551499
2210	9	-0.23505040918826836
2210	10	-0.13220272985685
2210	11	-0.2126824962188678
2210	12	-0.08244940903442455
2210	13	-0.24823220131052182
2210	14	-0.2084824459536847
2210	15	-0.32882418043704575
2210	16	0.0373184887924225
2210	17	0.2494216460143477
2210	18	0.1679684658570153
2210	19	-0.2792790699696184
2210	20	0.24119225473260997
2210	21	0.08439214473327183
2210	22	-0.038147146619984085
2210	23	-0.27793987029943423
2210	24	0.17784565719598788
2210	25	0.4950285037609916
2211	1	-0.07086000056241204
2211	2	0.028147610602253792
2211	3	0.03439478012681363
2211	4	0.23884039345508512
2211	5	-0.05323725093256826
2211	6	-0.02985620572727754
2211	7	-0.024170942034920984
2211	8	0.08712954292349906
2211	9	-0.38379302522201897
2211	10	-0.22207614757837035
2211	11	-0.319551694531107
2211	12	-0.0957658647306232
2211	13	-0.28787777093077693
2211	14	0.10270742746403272
2211	15	0.11529396302286443
2211	16	-0.2060907939080039
2211	17	0.3514132071957832
2211	18	-0.24404841178011338
2211	19	0.35169139416540673
2211	20	-0.1148752558159174
2211	21	0.061759233340865194
2211	22	0.34838449894963475
2211	23	0.05608544615625988
2211	24	-0.26090539765633025
2211	25	0.3192141577694301
2212	1	0.13819720916102085
2212	2	0.37321670983524413
2212	3	-0.21217588216669725
2212	4	-0.06709240348370571
2212	5	-0.025102161505010656
2212	6	0.37741074451102463
2212	7	0.15627286465805668
2212	8	0.06104436893692622
2212	9	0.40376740457691795
2212	10	0.040671764070275174
2212	11	0.5170017143074475
2212	12	0.12388257477124398
2212	13	-0.05987592600688174
2212	14	-0.03960795202656442
2212	15	-0.13394158934393374
2212	16	-0.5777746286164884
2212	17	-0.6059143863743159
2212	18	-0.17894651390006544
2212	19	0.23383833893726802
2212	20	0.06456067578523772
2212	21	0.027371901818291633
2212	22	-0.0687220454054085
2212	23	-0.18124351402413197
2212	24	-0.09059158976757686
2212	25	0.08487465760714485
2213	1	0.24621276340083043
2213	2	-0.30240057905435336
2213	3	-0.21558437298937605
2213	4	0.030521652940414867
2213	5	-0.027470502546115938
2213	6	-0.20712142812183743
2213	7	-0.158908521461548
2213	8	-0.28721378023655575
2213	9	-0.7282836107692958
2213	10	-0.46303276938254356
2213	11	0.11446374884042143
2213	12	-0.2285521758328457
2213	13	-0.04872231988760234
2213	14	-0.3298737503015161
2213	15	-0.11592299466629896
2213	16	-0.23174079974512551
2213	17	-0.7653293421279415
2213	18	-0.3431461586489135
2213	19	-0.13991148103681184
2213	20	-0.2859698006033611
2213	21	-0.4291098315513153
2213	22	-0.13082698851722085
2213	23	-0.4395367082440984
2213	24	-0.3150239080214021
2213	25	-0.5195643816145008
2214	1	-0.03406999731670979
2214	2	0.32058513088787777
2214	3	0.3017917291693344
2214	4	0.20922338598997925
2214	5	-0.38906977284104016
2214	6	0.1834836190049458
2214	7	0.14817569866615088
2214	8	0.04282574545514706
2214	9	-0.027204255342113015
2214	10	-0.22451042216454198
2214	11	0.18947134993497627
2214	12	-0.08583402441904298
2214	13	-0.41777066284898723
2214	14	-0.1586362921075377
2214	15	-0.19851648812935707
2214	16	0.2863954118693428
2214	17	0.18619087678357715
2214	18	-0.08218538029682776
2214	19	-0.10543291612346195
2214	20	-0.22511543757508434
2214	21	0.17008445242557713
2214	22	0.3150836226107856
2214	23	0.37867551431595103
2214	24	0.44199950334983384
2214	25	0.43102850376099155
2215	1	0.8810883737240118
2215	2	0.3866484793041671
2215	3	0.4068445195162411
2215	4	0.6058297208316077
2215	5	0.6985622030262277
2215	6	0.5963041318254625
2215	7	0.36416363230114257
2215	8	0.1741982944747562
2215	9	0.4748319437529105
2215	10	0.49328746622158093
2215	11	0.8197880920164238
2215	12	0.4315113752792996
2215	13	0.3339638771208513
2215	14	0.6953606913012109
2215	15	0.48466601564138045
2215	16	0.9780243711453593
2215	17	0.8603236067986586
2215	18	0.6482821913472137
2215	19	0.4936621065009703
2215	20	1.1407216664973134
2215	21	0.8065097917920951
2215	22	0.9693822651447164
2215	23	0.8822954238182135
2215	24	0.9693750689606944
2215	25	1.0878912488590302
2216	1	-0.05914692039363345
2216	2	-0.18464563834289294
2216	3	-0.11190057852297386
2216	4	-0.22969969093310283
2216	5	-0.29030054207181166
2216	6	-0.13036253484120408
2216	7	-0.27920891671845993
2216	8	-0.6971742545448549
2216	9	-0.5190504091882673
2216	10	-0.6242027298568473
2216	11	-0.5846824962188677
2216	12	-0.1984494090344242
2216	13	-0.7082322013105227
2216	14	-1.0404824459536854
2216	15	-0.6768241804370518
2216	16	-0.6786815112075786
2216	17	-0.2305783539856492
2216	18	-0.500031534142984
2216	19	-0.5672790699696151
2216	20	-0.15080774526739305
2216	21	-0.3836078552667317
2216	22	-0.25814714661999005
2216	23	-0.48593987029943264
2216	24	-0.6261543428040142
2216	25	-0.816971496239006
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	147.0
3	24.0
4	13.0
5	14.0
6	12.0
7	41.0
8	24.0
9	41.0
10	94.0
11	125.0
12	250.0
13	425.0
14	767.0
15	1245.0
16	1702.0
17	2057.0
18	1986.0
19	1787.0
20	1669.0
21	1585.0
22	1393.0
23	1216.0
24	1126.0
25	1039.0
26	962.0
27	935.0
28	884.0
29	832.0
30	973.0
31	1152.0
32	1539.0
33	1986.0
34	2664.0
35	4002.0
36	7175.0
37	17313.0
38	89464.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.05282419741126	25.24286214041603	11.051236511396356	21.65307715077635
2	12.929704084589622	31.11054202861437	30.17380228726236	25.785951599533654
3	20.455341978270834	36.602593481249826	24.541018521014745	18.40104601946459
4	13.206860531724903	28.48131549684941	25.995215149779288	32.31660882164639
5	10.825857502005162	45.22434234027782	29.903549980790878	14.046250176926137
6	27.920454468876564	37.75784572048547	16.12205427479733	18.199645535840638
7	18.968770638739503	42.45548771447441	19.735689350747375	18.84005229603871
8	24.357264128062088	39.69668795645027	19.0105640445199	16.93548387096774
9	24.007062089785578	25.62703001388159	17.78258467095244	32.5833232253804
10	16.982429180904624	38.280391720754054	26.38049214469135	18.356686953649973
11	24.06858451795459	33.99245435558849	16.437377888567	25.501583237889914
12	21.22263622091809	39.58643551614707	23.443406078818462	15.747522184116375
13	27.133825789232468	29.390990213786967	20.466125684091338	23.009058312889223
14	20.116580747329763	36.080730482832976	21.658411671552276	22.144277098284984
15	30.69054742459772	29.81523409072532	19.69151774884774	19.802700735829223
16	16.44485474969508	34.924763643959864	28.48401943409322	20.146362172251834
17	22.97273621649304	36.37282516408135	22.736216493039176	17.91822212638644
18	22.602578184489115	29.90586189933894	27.16190809911118	20.32965181706076
19	21.695456505335777	36.40118033847149	26.688315188099605	15.215047968093135
20	22.898906238417112	30.94273757135059	24.99679891095581	21.161557279276487
21	20.073189470420942	33.48991117520993	27.092234907198986	19.344664447170143
22	22.263319589295822	33.15681677311558	20.062252405206564	24.51761123238203
23	18.724815029447043	32.06627943019636	26.556245872697133	22.652659667659467
24	21.464289083875837	37.300688744221155	22.306686614640196	18.928335557262816
25	21.48981416264832	29.82946849433819	21.461369670043887	27.219347672969608
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6082.0
1	6082.0
2	4421.0
3	2760.0
4	2760.0
5	2760.0
6	2472.0
7	2184.0
8	2184.0
9	2184.0
10	2041.0
11	1898.0
12	1898.0
13	1898.0
14	1902.5
15	1907.0
16	1907.0
17	1907.0
18	2242.5
19	2578.0
20	2578.0
21	2578.0
22	3142.0
23	3706.0
24	3706.0
25	3706.0
26	4329.5
27	4953.0
28	4953.0
29	4953.0
30	5376.0
31	5799.0
32	5799.0
33	5799.0
34	6363.5
35	6928.0
36	6928.0
37	6928.0
38	6844.0
39	6760.0
40	6760.0
41	6760.0
42	8509.0
43	10258.0
44	10258.0
45	10258.0
46	25050.0
47	39842.0
48	39842.0
49	39842.0
50	34021.5
51	28201.0
52	28201.0
53	28201.0
54	20275.5
55	12350.0
56	12350.0
57	12350.0
58	8908.5
59	5467.0
60	5467.0
61	5467.0
62	4497.0
63	3527.0
64	3527.0
65	3527.0
66	2650.0
67	1773.0
68	1773.0
69	1773.0
70	1395.0
71	1017.0
72	1017.0
73	1017.0
74	727.5
75	438.0
76	438.0
77	438.0
78	304.0
79	170.0
80	170.0
81	170.0
82	111.5
83	53.0
84	53.0
85	53.0
86	30.0
87	7.0
88	7.0
89	7.0
90	5.5
91	4.0
92	4.0
93	4.0
94	2.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012780584274500042
2	0.18565480314536906
3	0.19574473809892173
4	0.18700012780584274
5	0.19910804975010596
6	0.181618829163948
7	0.1863274654756059
8	0.15807564760565843
9	0.17825551751276378
10	0.19641740042915856
11	0.15673032294518474
12	0.16547493323826373
13	0.19574473809892173
14	0.1802735045034743
15	0.1742195435313427
16	0.17758285518252692
17	0.17556486819181638
18	0.17758285518252692
19	0.15538499828471106
20	0.18565480314536906
21	0.18901811479655328
22	0.15807564760565843
23	0.17556486819181638
24	0.1863274654756059
25	0.6773709665485023
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	148663.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.92908793714643
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.54123711340206	27.326907165871805
2	11.022997620935765	7.480005112233709
3	3.455590800951626	3.517351324808459
4	1.486915146708961	2.0179869907105332
5	0.7474226804123711	1.2679684924964516
6	0.42426645519429024	0.8636984320241082
7	0.30729579698651865	0.7298386283069762
8	0.22601110229976212	0.6134680451760021
9	0.1744647105471848	0.5327485655475808
>10	1.2628865979381443	8.692142631320502
>50	0.1823949246629659	4.229028070199041
>100	0.0971451229183188	5.930191103368021
>500	0.04163362410785091	10.342183327391483
>1k	0.023790642347343377	14.83422236871313
>5k	0.005947660586835844	11.622259741832197
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	5868	3.947182553829803	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	5764	3.877225671485171	No Hit
CCCATGTACTCTGCGTTGATACCAC	5646	3.7978515165172233	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3070	2.0650733538271124	No Hit
CATGTACTCTGCGTTGATACCACTG	2455	1.6513860207314532	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2114	1.4220081661206891	No Hit
GCGTTGATACCACTGCTTCCCATGT	2098	1.4112455688368994	No Hit
GGTATCAACGCAGAGTACATGGGAA	1941	1.3056375829897149	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1835	1.2343353759846096	No Hit
TATCAACGCAGAGTACATGGGAAGC	1821	1.2249181033612937	No Hit
GTATCAACGCAGAGTACATGGGAAG	1657	1.1146014812024512	No Hit
GTGGTATCAACGCAGAGTACATGGG	1497	1.006975508364556	No Hit
GTACTCTGCGTTGATACCACTGCTT	1272	0.8556264840612661	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1195	0.803831484633029	No Hit
GATACCACTGCTTCCCATGTACTCT	1098	0.7385832386000551	No Hit
ATACCACTGCTTCCCATGTACTCTG	952	0.6403745383854759	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	909	0.6114500581852916	No Hit
CAGTGGTATCAACGCAGAGTACATG	898	0.6040507725526864	No Hit
CATGGGAAGCAGTGGTATCAACGCA	897	0.6033781102224495	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	879	0.5912701882781862	No Hit
GTATCAACGCAGAGTACTTTTTTTT	867	0.5831982403153441	No Hit
GGTATCAACGCAGAGTACTTTTTTT	814	0.5475471368127913	No Hit
GTTGATACCACTGCTTCCCATGTAC	808	0.5435111628313702	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	797	0.536111877198765	No Hit
TACCACTGCTTCCCATGTACTCTGC	781	0.5253492799149755	No Hit
GCAGTGGTATCAACGCAGAGTACAT	762	0.5125686956404754	No Hit
CTTCCCATGTACTCTGCGTTGATAC	749	0.5038240853473964	No Hit
CCACTGCTTCCCATGTACTCTGCGT	721	0.4849895401007648	No Hit
ACATGGGAAGCAGTGGTATCAACGC	686	0.4614463585424753	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	618	0.4157053200863698	No Hit
TATCAACGCAGAGTACTTTTTTTTT	599	0.40292473581186977	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	557	0.3746729179419223	No Hit
CCATGTACTCTGCGTTGATACCACT	543	0.3652556453186065	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	530	0.35651103502552756	No Hit
TTCCCATGTACTCTGCGTTGATACC	507	0.3410398014300801	No Hit
TGATACCACTGCTTCCCATGTACTC	501	0.3370038274486591	No Hit
ATCAACGCAGAGTACATGGGAAGCA	429	0.2885721396716062	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	383	0.2576296724807114	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	352	0.2367771402433692	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	320	0.21525194567579023	No Hit
TCAACGCAGAGTACATGGGAAGCAG	284	0.1910361017872638	No Hit
GTACATGGTAAGCAGTGGTATCAAC	277	0.1863274654756059	No Hit
AAAAACAAAAAAAAAAAAAAAAAAA	268	0.1802735045034743	No Hit
CTCTGCGTTGATACCACTGCTTCCC	265	0.17825551751276378	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	249	0.16749292022897427	No Hit
CTGCGTTGATACCACTGCTTCCCAT	248	0.1668202578987374	No Hit
AAAAAGTACTCTGCGTTGATACCAC	220	0.14798571265210578	No Hit
AGTGGTATCAACGCAGAGTACATGG	213	0.14327707634044787	No Hit
ACACAAAAAAAAAAAAAAAAAAAAA	211	0.14193175167997418	No Hit
ACCATGTACTCTGCGTTGATACCAC	203	0.13655045303807942	No Hit
ACTGCTTCCCATGTACTCTGCGTTG	198	0.1331871413868952	No Hit
AACGCAGAGTACATGGGAAGCAGTG	191	0.12847850507523728	No Hit
ATGTACTCTGCGTTGATACCACTGC	189	0.1271331804147636	No Hit
GTGGTATCAACGCAGAGTACTTTTT	184	0.12376986876357937	No Hit
TCCATGTACTCTGCGTTGATACCAC	163	0.10964395982860564	No Hit
CTGTACTTGTTCGCTATCGGTCTCT	160	0.10762597283789511	No Hit
CCCCATGTACTCTGCGTTGATACCA	160	0.10762597283789511	No Hit
AAAGTACTCTGCGTTGATACCACTG	159	0.10695331050765826	No Hit
TCCCATGTACTCTGCGTTGATACCA	158	0.10628064817742142	No Hit
TTGATACCACTGCTTCCCATGTACT	157	0.10560798584718459	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA	157	0.10560798584718459	No Hit
GGTATCAACGCAGAGTACATGGGGG	156	0.10493532351694773	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA	151	0.1015720118657635	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACAAA	90	3.5255653E-8	13.730011	1
GAAAAAA	365	0.0	10.67729	1
ATGGTAA	100	2.711035E-4	9.502191	5
CATGGTA	100	2.711035E-4	9.502191	4
TGGTAAG	100	2.738729E-4	9.492595	6
GGTAAGC	110	7.078324E-4	8.632538	7
CACAAAA	185	5.8949627E-7	8.21811	2
ACATGGT	120	0.0016644867	7.918492	3
TAAGCAG	120	0.0016755906	7.9131594	9
GTAAGCA	120	0.0016755906	7.9131594	8
ACACAAA	175	2.0663321E-5	7.6043143	1
AGTGGTA	1975	0.0	7.25763	14
CAGTGGT	1970	0.0	7.230298	13
AGCAGTG	1990	0.0	7.1099143	11
AAGCAGT	1995	0.0	7.0444965	10
GAAGCAG	1815	0.0	6.9583483	9
TGGTATC	2090	0.0	6.903705	16
GTGGTAT	2105	0.0	6.8094153	15
GGAAGCA	1860	0.0	6.7900014	8
CCATGTA	1825	0.0	6.7166166	2
>>END_MODULE