FastQCFastQC Report
Wed 25 May 2016
SRR1294821_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294821_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148663
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC60294.055481188997935No Hit
CCCATGTACTCTGCGTTGATACCAC58923.9633264497554874No Hit
GTATCAACGCAGAGTACTTTTTTTT34432.3159764030054553No Hit
GAGTACATGGGAAGCAGTGGTATCA33142.2292029624049023No Hit
GGTATCAACGCAGAGTACTTTTTTT32562.1901885472511653No Hit
CATGTACTCTGCGTTGATACCACTG25301.7018356954992164No Hit
TATCAACGCAGAGTACTTTTTTTTT24451.6446593974290844No Hit
ACGCAGAGTACATGGGAAGCAGTGG23111.5545226451773475No Hit
GCGTTGATACCACTGCTTCCCATGT21231.4280621270928207No Hit
GGTATCAACGCAGAGTACATGGGAA20311.366177192711031No Hit
ACTCTGCGTTGATACCACTGCTTCC19371.3029469336687676No Hit
TATCAACGCAGAGTACATGGGAAGC17251.1603425196585566No Hit
GTATCAACGCAGAGTACATGGGAAG16811.1307453771281355No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16561.1139288188722143No Hit
GTGGTATCAACGCAGAGTACATGGG14931.0042848590436086No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13290.8939682368847661No Hit
GTACTCTGCGTTGATACCACTGCTT12360.8314106401727396No Hit
GCTTCCCATGTACTCTGCGTTGATA12000.8071947962842133No Hit
GATACCACTGCTTCCCATGTACTCT11320.7614537578281079No Hit
CAGTGGTATCAACGCAGAGTACATG10430.7015868104370288No Hit
GGGAAGCAGTGGTATCAACGCAGAG9840.6618997329530549No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9830.661227070622818No Hit
CATGGGAAGCAGTGGTATCAACGCA9820.6605544082925812No Hit
ATACCACTGCTTCCCATGTACTCTG9730.6545004473204495No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA9510.639701876055239No Hit
CTGCTTCCCATGTACTCTGCGTTGA9480.6376838890645286No Hit
GCAGAGTACATGGGAAGCAGTGGTA9120.6134680451760021No Hit
GTTGATACCACTGCTTCCCATGTAC8200.5515831107942124No Hit
ACATGGGAAGCAGTGGTATCAACGC8100.5448564874918439No Hit
TACCACTGCTTCCCATGTACTCTGC7850.5280399292359229No Hit
GCAGTGGTATCAACGCAGAGTACAT7820.5260219422452124No Hit
AAGCAGTGGTATCAACGCAGAGTAC7370.4957521373845543No Hit
CTTCCCATGTACTCTGCGTTGATAC7010.47153629349602794No Hit
CCACTGCTTCCCATGTACTCTGCGT6960.46817298184484374No Hit
GTGGTATCAACGCAGAGTACTTTTT6660.44799311193773833No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6590.4432844756260805No Hit
ATGGGAAGCAGTGGTATCAACGCAG6370.42848590436086986No Hit
TGATACCACTGCTTCCCATGTACTC5470.36794629463955386No Hit
CCATGTACTCTGCGTTGATACCACT5450.3666009699790802No Hit
TTCCCATGTACTCTGCGTTGATACC4430.29798941229492204No Hit
ATCAACGCAGAGTACATGGGAAGCA4130.27780954238781674No Hit
GGAAGCAGTGGTATCAACGCAGAGT3940.26502895811331667No Hit
ATCAACGCAGAGTACTTTTTTTTTT3720.2502303868481061No Hit
GTACATGGTAAGCAGTGGTATCAAC3010.20247136140129018No Hit
CTGCGTTGATACCACTGCTTCCCAT2950.19843538741986913No Hit
TCAACGCAGAGTACATGGGAAGCAG2740.18430947848489537No Hit
TGGGAAGCAGTGGTATCAACGCAGA2740.18430947848489537No Hit
AAAAAGTACTCTGCGTTGATACCAC2560.17220155654063218No Hit
CTCTGCGTTGATACCACTGCTTCCC2440.16412960857779005No Hit
AGTGGTATCAACGCAGAGTACATGG2400.16143895925684265No Hit
AACGCAGAGTACTTTTTTTTTTTTT2190.14731305032186892No Hit
ACCATGTACTCTGCGTTGATACCAC2180.1466403879916321No Hit
CAACGCAGAGTACTTTTTTTTTTTT2110.14193175167997418No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT2060.13856844002878996No Hit
AACGCAGAGTACATGGGAAGCAGTG1910.12847850507523728No Hit
ACTTAGAGCTGCATTCCCAAACAAC1870.1257878557542899No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT1850.12444253109381621No Hit
ACTGCTTCCCATGTACTCTGCGTTG1850.12444253109381621No Hit
ATGTACTCTGCGTTGATACCACTGC1800.12107921944263199No Hit
TCAACGCAGAGTACTTTTTTTTTTT1800.12107921944263199No Hit
AAAGTACTCTGCGTTGATACCACTG1780.1197338947821583No Hit
GGTATCAACGCAGAGTACATGGGGT1720.11569792080073724No Hit
TCCATGTACTCTGCGTTGATACCAC1700.11435259614026355No Hit
TCCCATGTACTCTGCGTTGATACCA1650.11098928448907933No Hit
CTGTACTTGTTCGCTATCGGTCTCT1620.1089712974983688No Hit
GGTATCAACGCAGAGTACATGGGGG1590.10695331050765826No Hit
CCCCATGTACTCTGCGTTGATACCA1520.10224467419600033No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA1490.10022668720528981No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACG250.006060728318.97073716
ATCGCAC250.006060728318.97073715
CGCACGG250.006060728318.97073717
CACGGGA250.006060728318.97073719
TCACCCA350.0021801416.26063212
GAACAAA508.278007E-515.2742051
CACCCAG400.00530340214.22805213
CTCGACT500.001505841413.27951612
GACTCTT500.001505841413.27951615
CGACTCT500.001505841413.27951614
ACTCGAC550.003081431812.07228711
TCGACTC550.003081431812.07228713
TCCCAAA1200.00160062087.9499322
GAAGCAG17450.07.06646359
AACAAAA1500.00181082656.99594072
GGAAGCA17750.06.94703058
CATGGGT1656.460954E-46.93812274
AGCAGTG19550.06.74407311
CAGTGGT19550.06.695554313
AAGCAGT19550.06.695554310
Produced by FastQC (version 0.11.5)