FastQCFastQC Report
Wed 25 May 2016
SRR1294820_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294820_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2699657
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC40540.15016722494746554No Hit
CCCATGTACTCTGCGTTGATACCAC34960.12949793251513061No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG402.7748127E-416.61496417
TACGACC905.392285E-712.6700724
GCGTGCT701.0934661E-412.2118999
ACGGATC700.001494451510.8550218
CTTTGCG1201.536644E-610.28545417
GGTACGA750.002644786110.1351162
GACCGAA856.614923E-410.05573711
CTTACAC3800.010.0013893
CTTGGAC2600.09.8667543
TTGCGGA1401.0023887E-69.53852319
AGTACCG1201.6966485E-59.5022015
GTATAGG2550.09.315711
ATTAGAC2151.05501385E-109.2803583
GTCTTAG2501.8189894E-129.1219431
CTAAGAC3050.09.034043
GTCTAAG2002.6484486E-99.0269231
TAGACTG2950.09.0190385
TGGACAG6900.08.9513485
CAAACGC850.00744964748.93942810
GTGTAAG4150.08.9296131
Produced by FastQC (version 0.11.5)