FastQCFastQC Report
Wed 25 May 2016
SRR1294820_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294820_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2699657
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74090.27444227174044705No Hit
GGTATCAACGCAGAGTACTTTTTTT48090.17813374069372517No Hit
TATCAACGCAGAGTACTTTTTTTTT42420.15713107257699774No Hit
GTACATGGGAAGCAGTGGTATCAAC40970.15176001988400747No Hit
CCCATGTACTCTGCGTTGATACCAC37570.1391658273625131No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA604.1360836E-412.6512317
ACCGTCC604.139316E-412.650068
ACCGTAT951.3810468E-510.9855788
AGACCGT1053.5033681E-610.8481326
TACGTCG750.002574905310.1678815
GTACCGT750.002666955810.1249246
AGTACCG1252.4621331E-69.9136845
TCTATAC2700.09.8854393
ACCGTGT2500.09.8670468
GTCTTAG2450.09.7287371
GTCCTAG3300.09.5341621
TGTACCG1106.589676E-59.5323885
GCACCGT1856.7993824E-99.2355726
CTACGTC1356.5613185E-69.1793374
CTTAGAC2106.2573235E-109.0784653
CCAGGAC6000.09.0557693
ACGTCGC1054.5316265E-49.040116
CCCCTAG1606.4040614E-78.9382771
TTAGGAC2605.456968E-128.7991273
GTCTTAC2409.458745E-118.7396491
Produced by FastQC (version 0.11.5)