FastQCFastQC Report
Wed 25 May 2016
SRR1294819_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294819_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4521422
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG551.9592125E-413.82004453
GGTATCA17800.011.2097481
GTCGTAA700.00149026510.8589681
CGACGTA700.001495372910.8545149
TCGTCCG700.001495372910.8545149
AGCGTAA803.782125E-410.68491310
GCGACGT909.546773E-510.55299958
GTATCAA34950.09.8142391
TCGACCG800.00452729459.49779
GAACCGA1356.8950103E-69.1439076
CGCTCGA850.0074541238.93901210
GTAGACC3000.08.8678623
GTATTAA8500.08.8308961
GTTCTAG3950.08.6596831
TAGACCG1107.136243E-48.6379115
ACGACTG1000.00290191038.5515325
GTGTTAG5150.08.4868631
GTATAAA8650.08.4580691
CTTATAC5200.08.4049793
ACCGTCT2159.775249E-98.3933168
Produced by FastQC (version 0.11.5)