FastQCFastQC Report
Wed 25 May 2016
SRR1294819_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294819_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4521422
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT94100.2081203656725694No Hit
TATCAACGCAGAGTACTTTTTTTTT56200.12429717907330923No Hit
GGTATCAACGCAGAGTACTTTTTTT55190.12206336855971418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23050.011.8704031
TTACGCA600.005739151511.1191364
ACGAGCG803.662866E-410.7220235
CGAGCGT909.5997755E-510.5472386
GTCGCGC750.002677160810.1205358
GTATCAA47150.010.0491471
TACGTCG1053.9658542E-59.98452955
GTATACG1159.853673E-69.9480491
TAACGTG1252.4691417E-69.9119155
GTCTACG1606.294067E-89.5335461
ACTGCGT1856.85759E-99.2315698
CGCACGA1054.551808E-49.03639112
CTTATAC5700.09.0290723
TAGACCG850.00724510068.9700595
ATCGTCG1304.4379398E-58.7581559
GGACGGT1201.7802998E-48.7014726
GCCCTAG3400.08.6923511
CTAGGAC3550.08.59104253
GACCGTG2106.526534E-98.5851427
TCGACCG1000.00294005968.5392029
Produced by FastQC (version 0.11.5)