FastQCFastQC Report
Wed 25 May 2016
SRR1294818_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294818_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2415543
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC51240.21212621758337566No Hit
CCCATGTACTCTGCGTTGATACCAC45710.18923281431959604No Hit
ATTCCATTCCATTCCATTCCATTCC25030.10362059379609471No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTCG701.09824476E-412.20608616
GTTCTAG2350.010.9173031
AGCGTCT700.001494857710.8545828
GTCTAGC1106.822392E-59.5020961
TCTAACC2002.6375346E-109.5015053
TATACTG4750.09.4024655
GTGTAGG3650.09.3719311
GCTACAC1856.682967E-99.2447073
TAAGACT2750.08.9841734
CTATACT4100.08.8071874
CCTATAC2401.0186341E-108.7097133
GTATAAG3550.08.565271
GTCTTAG2356.148184E-108.4912361
TACACTG5600.08.4843675
CTTATAC3050.08.4111693
GTGTTAG3850.08.3914621
GTCTAGA2502.3464963E-108.3618461
GCTGGAC2752.1827873E-118.2922223
TTATACT4950.08.2546894
GCCTTAC1502.5794248E-58.2351511
Produced by FastQC (version 0.11.5)