FastQCFastQC Report
Wed 25 May 2016
SRR1294818_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294818_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2415543
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65190.26987720773341645No Hit
GTACATGGGAAGCAGTGGTATCAAC51350.2125816017350964No Hit
CCCATGTACTCTGCGTTGATACCAC46000.19043337253776893No Hit
GGTATCAACGCAGAGTACTTTTTTT41340.171141643928508No Hit
TATCAACGCAGAGTACTTTTTTTTT38700.16021242428720994No Hit
ATTCCATTCCATTCCATTCCATTCC24750.10246143413716917No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCCG307.5426244E-419.06745
AAACCGT500.001511124713.2869256
AGACCGT500.001511124713.2869256
GCCCTAG1850.011.3418551
CGGACAC600.0057243511.122653
AGGCTCG700.001448486610.8956575
TACACCG909.1834285E-510.5929995
GTCTAGC1553.436071E-910.4604491
GCGCTCA750.002679362210.119189
TACCGTG856.673424E-410.0460227
GTCTTAG2105.456968E-129.9916341
ACACCGT1356.533446E-79.8421676
TAGGACC1659.651558E-99.8225994
GTATTAG4000.09.5374691
TATTCCG800.00438692239.5336995
CGTGCAT800.00456935079.48673210
CTAAGAC2550.09.3467653
TTAGAAC2409.094947E-129.1364623
GTCTAAG2002.4756446E-99.06059551
CTACACT4350.08.98578554
Produced by FastQC (version 0.11.5)