FastQCFastQC Report
Wed 25 May 2016
SRR1294817_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294817_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3375732
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC72700.21536069806489377No Hit
CCCATGTACTCTGCGTTGATACCAC67350.19951228355805498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGTA500.001504196613.29528311
AGTACGG1406.8030204E-1011.5369313
GCGGAAA600.005893609511.07940313
TACGACG803.7673942E-410.6892624
CGCGTCC803.7824118E-410.68466110
GCGCTGA2450.010.4647436
AGACCGT1201.5339192E-610.2871016
ATACCGT951.6524992E-49.99556356
ATACGAC1151.0259922E-59.914093
CGGGACT1201.6983908E-59.5015674
GTACTAG2106.730261E-119.5014261
GTAGCAC2401.8189894E-129.5010033
CGTGCGT900.00111693439.49747710
CGTGCAT800.00452767139.49747710
CCGTAAT800.00453765889.4949414
TTAGACT2750.09.3288114
GTGTAGA4800.09.303481
CTAGGAC4100.09.269273
ACTGCGT1356.877104E-69.1455828
GCTGCGC2600.09.1355793
Produced by FastQC (version 0.11.5)