FastQCFastQC Report
Wed 25 May 2016
SRR1294817_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294817_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3375732
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT93390.27665110855956576No Hit
GTACATGGGAAGCAGTGGTATCAAC73250.21698997432260617No Hit
CCCATGTACTCTGCGTTGATACCAC69490.20585164936079048No Hit
GGTATCAACGCAGAGTACTTTTTTT56730.16805244018186277No Hit
TATCAACGCAGAGTACTTTTTTTTT55870.1655048445788943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCCG704.5079832E-714.9086839
CGTCCGC759.829018E-713.91476910
GACGCTT752.0918105E-411.39021119
TACCGTG855.392408E-511.1630697
AAAAGCG600.005730404511.121285
ATACCGT803.803209E-410.6783236
ATCGTCC1106.1478586E-610.3498298
GTACCGT951.6594394E-49.9914136
ACCGCTC951.667396E-49.9866778
AGGACCG1002.6639417E-49.5325255
TCTACGC900.0010799289.5325253
GTATTAG5500.09.3617141
CTTAGAC2304.0017767E-119.1180683
GTCCGCA1252.7986676E-59.10811911
GTCGTGC1151.1275663E-49.0748518
TAGACTG4100.09.0675245
GACGTAG1054.5336757E-49.039856
GGACCGG1054.5336757E-49.039856
TTGACGC1054.5538595E-49.03583317
AAGCGTC950.0018468768.98800918
Produced by FastQC (version 0.11.5)