FastQCFastQC Report
Wed 25 May 2016
SRR1294816_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294816_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39061
Sequences flagged as poor quality0
Sequence length25
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA31558.077110161030184No Hit
GTATCAACGCAGAGTACTTTTTTTT4231.0829215841888329No Hit
GGTATCAACGCAGAGTACTTTTTTT4091.0470802078799826No Hit
TATCAACGCAGAGTACTTTTTTTTT2610.6681856583292799No Hit
AAACAAAAAAAAAAAAAAAAAAAAA2580.6605053634059548No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1540.3942551393973529No Hit
GTACATGGGAAGCAGTGGTATCAAC1400.3584137630885026No Hit
GTGGTATCAACGCAGAGTACATGGG1260.32257238677965233No Hit
CCCATGTACTCTGCGTTGATACCAC1230.31489209185632727No Hit
AAAAACAAAAAAAAAAAAAAAAAAA1080.276490617239702No Hit
ACACAAAAAAAAAAAAAAAAAAAAA1060.27137042062415195No Hit
GTGGTATCAACGCAGAGTACTTTTT740.18944727477535137No Hit
GGTATCAACGCAGAGTACATGGGGG730.18688717646757635No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA680.17408668492870127No Hit
GAGTACTTTTTTTTTTTTTTTTTTT650.16640639000537621No Hit
GTACTTTTTTTTTTTTTTTTTTTTT640.16384629169760118No Hit
GAGTACATGGGAAGCAGTGGTATCA590.1510458001587261No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA580.14848570185095109No Hit
GGTATCAACGCAGAGTACATGGGAA570.14592560354317605No Hit
GTACTGGTTCACTATCGGTCAGTCA550.14080540692762603No Hit
GGTATCAACGCAGAGTACATGGGGA540.138245308619851No Hit
GGTATCAACGCAGAGTACATGGGCA540.138245308619851No Hit
ACGCAGAGTACATGGGAAGCAGTGG530.13568521031207598No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA510.13056501369652596No Hit
GCAGAGTACTTTTTTTTTTTTTTTT510.13056501369652596No Hit
ATACAGGGTGACAGCCCCGTACACA510.13056501369652596No Hit
TATCAACGCAGAGTACATGGGAAGC500.12800491538875092No Hit
GGATACCACGTGTCCCGCCCTACTC450.11520442384987584No Hit
CATGTACTCTGCGTTGATACCACTG420.10752412892655078No Hit
AAAAAAAACAAAAAAAAAAAAAAAA410.10496403061877575No Hit
GTATCAACGCAGAGTACATGGGAAG400.10240393231100076No Hit
ATCAACGCAGAGTACTTTTTTTTTT400.10240393231100076No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAAAA856.295075E-410.0581441
GGTATCA1553.7718564E-79.1929271
GTATCAA2102.857516E-56.7852562
TATCAAC2401.8119317E-45.93709853
TACATGG2752.9563009E-55.872332
GTACATG2855.1035495E-55.6662841
ACATGGG2906.649432E-55.5685893
AACGCAG2605.3775916E-45.48039877
ATCAACG2656.950553E-45.3769954
TCAACGC2708.9326536E-45.2774215
ACAAAAA3101.8196156E-45.20932533
CAACGCA2750.00116160145.1748256
Produced by FastQC (version 0.11.5)