##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294816_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39061
Sequences flagged as poor quality	0
Sequence length	25
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.63518599114206	31.0	28.0	34.0	15.0	34.0
2	28.923401858631372	33.0	28.0	34.0	15.0	34.0
3	29.22664550318732	33.0	28.0	34.0	16.0	34.0
4	31.807480607255318	37.0	32.0	37.0	12.0	37.0
5	32.77499295972965	37.0	32.0	37.0	19.0	37.0
6	32.63917974450219	37.0	32.0	37.0	17.0	37.0
7	32.80079875067202	37.0	32.0	37.0	18.0	37.0
8	32.792964849850236	37.0	32.0	37.0	18.0	37.0
9	33.72084688052021	39.0	32.0	39.0	17.0	39.0
10	34.07158034868539	39.0	33.0	39.0	17.0	39.0
11	33.930851744707	39.0	33.0	39.0	17.0	39.0
12	34.19231458488006	39.0	34.0	39.0	18.0	39.0
13	33.8117559714293	39.0	33.0	39.0	17.0	39.0
14	34.90304907708456	40.0	33.0	41.0	17.0	41.0
15	34.9224546222575	40.0	33.0	41.0	17.0	41.0
16	34.907503648140086	40.0	33.0	41.0	17.0	41.0
17	34.85625048001843	40.0	33.0	41.0	17.0	41.0
18	34.750390414991934	40.0	33.0	41.0	17.0	41.0
19	34.74386216430711	40.0	33.0	41.0	18.0	41.0
20	34.68687437597604	40.0	33.0	41.0	18.0	41.0
21	34.698266813445635	40.0	32.0	41.0	18.0	41.0
22	34.751619262179666	40.0	32.0	41.0	18.0	41.0
23	34.723918998489545	40.0	32.0	41.0	18.0	41.0
24	34.90033537287832	40.0	32.0	41.0	21.0	41.0
25	33.92237781930826	39.0	30.0	41.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8090324367241166
1101	2	0.6816218474148457
1101	3	0.41276956411494936
1101	4	-0.007411499950212175
1101	5	0.29428285660575426
1101	6	0.43718381257757954
1101	7	0.534558835007644
1101	8	0.7140550017392044
1101	9	0.46430812929105514
1101	10	0.6331654396727586
1101	11	0.7032769535164078
1101	12	1.4186694006542808
1101	13	1.5722654971125252
1101	14	0.7271378746904418
1101	15	2.089754443056762
1101	16	1.032334132269419
1101	17	0.39189384906733693
1101	18	1.0255099361497173
1101	19	0.058370347757772834
1101	20	-0.5166712547971386
1101	21	0.4507518998322908
1101	22	-0.8298448047593681
1101	23	-0.33718223746119236
1101	24	0.5743413494487015
1101	25	0.6777355688249287
1102	1	-1.1410907159852428
1102	2	-1.024658940762496
1102	3	-0.7239299432742143
1102	4	-0.17120460339848975
1102	5	0.008568570891469562
1102	6	-0.8115846603288261
1102	7	-0.2924116083421069
1102	8	-0.67757061402434
1102	9	-0.23950960469909433
1102	10	-0.16424835343068622
1102	11	-1.4020186129860548
1102	12	-1.0259118801338971
1102	13	-0.25162612850323995
1102	14	0.4660541308480788
1102	15	-1.0863539313274728
1102	16	0.07235876281129094
1102	17	0.0969431101510807
1102	18	-1.1025688815842756
1102	19	-0.3892897507643909
1102	20	-1.5936416981468895
1102	21	-0.05786878982288357
1102	22	-0.13341623333079866
1102	23	-0.15922657243656602
1102	24	-1.06358968503406
1102	25	-0.4885205888105375
1103	1	-1.4144423643368924
1103	2	-0.13866992977348502
1103	3	-1.5865673059115757
1103	4	-2.113512295706183
1103	5	-1.590332528009629
1103	6	-1.9434527921969575
1103	7	-2.1756533665838695
1103	8	-1.5319662184199458
1103	9	-0.3480260882155761
1103	10	-0.923863738046073
1103	11	-0.2522933382607775
1103	12	-0.8445931988152111
1103	13	-0.28184590872301385
1103	14	-0.3663634515695051
1103	15	-1.1138264588000055
1103	16	-0.47297090751838056
1103	17	-0.6956393074313354
1103	18	-0.7921293211447136
1103	19	-0.484069970544617
1103	20	-0.8422680717732689
1103	21	0.21136197940788293
1103	22	-0.5221524970670615
1103	23	-0.6276331658431573
1103	24	-1.1789743004186732
1103	25	-2.3209381712281214
1104	1	-1.021481551564758
1104	2	-1.0613840081479395
1104	3	-0.3728517761583099
1104	4	-0.43063856566264036
1104	5	-1.479302049054624
1104	6	-0.48409139887329644
1104	7	-0.11215554365208646
1104	8	-2.4619371908437486
1104	9	-1.451436828418771
1104	10	-2.322267221355215
1104	11	-1.3956169957353808
1104	12	-3.0198471901069404
1104	13	-1.443674646023453
1104	14	-1.0379889958365567
1104	15	-1.4509091873921633
1104	16	-1.1449592964878974
1104	17	-2.057032631088809
1104	18	-1.1713020352230856
1104	19	-1.7373355728668187
1104	20	-4.069046010815356
1104	21	-3.3361706766153354
1104	22	-3.35814669155182
1104	23	-3.3293748204150013
1104	24	-2.157929307675566
1104	25	-1.3746391871932886
1105	1	-1.7186668283974207
1105	2	-2.009729198373737
1105	3	-1.8498081634147283
1105	4	-2.314647226349308
1105	5	-1.7057171433942457
1105	6	-0.08910222473163287
1105	7	-1.6791212102156479
1105	8	-1.6400998880290274
1105	9	-1.5992871222634975
1105	10	-1.0392483534306862
1105	11	-1.6681193155621656
1105	12	-1.526497360227573
1105	13	-1.9735230840067501
1105	14	-3.208418937067613
1105	15	-1.7169159922174089
1105	16	-1.826060440935784
1105	17	-2.1281739858676545
1105	18	-1.789922511560853
1105	19	-1.0710813198510465
1105	20	-2.271334906577806
1105	21	-2.0701638717900934
1105	22	-2.2888611982019924
1105	23	-2.4428916778229848
1105	24	-2.194737226017665
1105	25	-2.478567427218035
1106	1	-1.0541859540804808
1106	2	-0.9758494169529719
1106	3	-2.0763108956551655
1106	4	-1.3212046033984883
1106	5	-0.6723838100609143
1106	6	-2.180632279376443
1106	7	-1.0269354178659178
1106	8	-1.9870944235481538
1106	9	-1.612128652318141
1106	10	-2.2392483534306855
1106	11	-1.5984471844146242
1106	12	-0.7830547372767498
1106	13	-1.0921023189794283
1106	14	-2.4791839643900175
1106	15	-2.279211074184616
1106	16	-1.6716888562363295
1106	17	-2.94948546127749
1106	18	-1.6882831672985574
1106	19	-1.4404802269548682
1106	20	-0.9281655076707054
1106	21	-1.7745354564895521
1106	22	-2.2298448047593666
1106	23	-1.9366075248175179
1106	24	-1.5802563517007258
1106	25	-2.433758684048634
1107	1	1.4780544830779867
1107	2	0.8263363753967568
1107	3	0.7239623283885486
1107	4	0.980434740863803
1107	5	1.195922200868047
1107	6	0.664996135924099
1107	7	0.7307148553581229
1107	8	0.7533427349217945
1107	9	0.5974341892119099
1107	10	0.07550574492996986
1107	11	-0.19270947949659245
1107	12	0.5226829676412805
1107	13	0.5182801946817719
1107	14	-0.09366483870695674
1107	15	0.6601331881104642
1107	16	0.04279201808061117
1107	17	0.06854732560776
1107	18	0.3740119146686496
1107	19	0.30596786047682656
1107	20	0.5319437819467865
1107	21	1.0773771118164603
1107	22	0.9078601132734221
1107	23	0.6718624205376713
1107	24	0.3626398231626595
1107	25	0.8493014252409807
1108	1	-0.3778418680589759
1108	2	-0.33283866426479847
1108	3	0.8371299645598889
1108	4	-0.8889465388823616
1108	5	-0.044426820813598056
1108	6	0.43495911847301727
1108	7	-0.10059133184441293
1108	8	-0.8526858213976141
1108	9	-1.2642791899525534
1108	10	0.02526777560157001
1108	11	-0.04199557151139999
1108	12	-1.2136998985670715
1108	13	0.8186503691926177
1108	14	-0.6238076203039995
1108	15	0.9078857000089329
1108	16	0.6697089932260383
1108	17	-1.188195138696841
1108	18	0.36978134883047176
1108	19	-0.31037270007315243
1108	20	-0.055047228100811196
1108	21	-0.35625588659707574
1108	22	-0.4266189983077524
1108	23	-0.9489731162153703
1108	24	-0.48294452374373975
1108	25	-0.31225330770455173
1109	1	0.5531057125861842
1109	2	1.5043589163803617
1109	3	0.505980771011501
1109	4	1.0475453966015102
1109	5	1.6380328566057543
1109	6	1.1370760539568892
1109	7	0.7678562488007472
1109	8	0.24207224311851405
1109	9	0.2805796810151904
1109	10	1.6170016465693138
1109	11	1.0265528155853758
1109	12	0.5075702627232488
1109	13	0.828731014353906
1109	14	1.7093577022766482
1109	15	1.7082889258153813
1109	16	0.8022694770970062
1109	17	1.1661395387225113
1109	18	0.7835918327014397
1109	19	-8.968936215367762E-4
1109	20	0.1385011589959646
1109	21	0.23900621017711643
1109	22	0.9826551952406319
1109	23	1.6800591418491493
1109	24	1.18641031496594
1109	25	1.911032982618032
1110	1	0.8670261671316375
1110	2	1.0635445224409672
1110	3	1.331264861920591
1110	4	1.5333408511469635
1110	5	0.20337376569666077
1110	6	0.5390646903205223
1110	7	-0.06784450877501058
1110	8	0.755329818876092
1110	9	1.0196895295000417
1110	10	-0.054399868582201805
1110	11	0.8030679671005245
1110	12	0.8669452627232488
1110	13	0.11092798405087478
1110	14	0.007179671973617019
1110	15	1.2026071076335612
1110	16	0.9722505377030686
1110	17	1.3323327205406912
1110	18	1.7026259236105332
1110	19	1.850428863954221
1110	20	1.547592068086871
1110	21	1.4497069677528742
1110	22	1.2459127709982099
1110	23	1.119453081243087
1110	24	0.93641031496594
1110	25	0.8177564674665163
1111	1	1.7616870617925322
1111	2	1.7987537576502035
1111	3	1.5181335487892795
1111	4	2.3010176188237317
1111	5	2.2149177772406716
1111	6	2.037621688877522
1111	7	1.1897312488007472
1111	8	1.7668738304201028
1111	9	2.4251729349834434
1111	10	2.262338948156618
1111	11	2.1356797997123564
1111	12	2.5653579611359447
1111	13	2.1253579984808866
1111	14	2.021609686403629
1111	15	1.2430111480376027
1111	16	1.8322793977319236
1111	17	1.3251177133256888
1111	18	1.6037803247649336
1111	19	2.791265804791159
1111	20	2.3289773494721544
1111	21	2.969908987954895
1111	22	2.51936154444698
1111	23	2.60863057042058
1111	24	2.428473807029434
1111	25	2.2138603635704115
1112	1	0.1587493693026012
1112	2	0.24106600593260197
1112	3	0.35229606951896386
1112	4	0.3176013667507611
1112	5	0.025626140187846147
1112	6	-1.2540153639535596
1112	7	-0.6460896467216415
1112	8	-0.4025173091202916
1112	9	0.5294136362390702
1112	10	-0.18850208477397246
1112	11	-0.37083524411611535
1112	12	-0.9091741402618254
1112	13	-0.41100779161624246
1112	14	0.06733344854530543
1112	15	0.1252665377556781
1112	16	0.76239074575372
1112	17	0.031111553647882317
1112	18	-0.3061936150597546
1112	19	0.12270385264712047
1112	20	-0.189857049959258
1112	21	0.9085491206248761
1112	22	0.15522982210630687
1112	23	-0.3871050372553313
1112	24	-0.8994105805564487
1112	25	-0.5402263457401801
1113	1	2.3830689478803
1113	2	2.119248622262713
1113	3	2.0638851827762075
1113	4	2.6817365730720972
1113	5	1.5736946213116383
1113	6	1.9762304657215921
1113	7	2.307378307624276
1113	8	1.370748713706746
1113	9	2.1270870339563643
1113	10	1.7695751759810818
1113	11	2.651552815585376
1113	12	2.3375334980173648
1113	13	2.302995720236261
1113	14	2.72865917286488
1113	15	2.3590242199330262
1113	16	2.5587033006264193
1113	17	2.6505145387225113
1113	18	2.3764227150543817
1113	19	2.931088400496108
1113	20	2.3002658648783196
1113	21	2.2215429748830005
1113	22	2.008390489358277
1113	23	2.0330003183197363
1113	24	2.127586785554172
1113	25	1.3917315120298
1114	1	-1.4589478588423859
1114	2	-0.9568017979053529
1114	3	-1.4310728004170699
1114	4	-1.2069188891127745
1114	5	-0.5628600005370998
1114	6	-0.34015608890025106
1114	7	-0.7531258940563959
1114	8	-0.8775706140243429
1114	9	-1.0002238904133804
1114	10	-1.5535340677163987
1114	11	-0.3770186129860562
1114	12	-1.0330547372767498
1114	13	-2.0730546999318094
1114	14	-1.0768030120090657
1114	15	-0.6363539313274771
1114	16	-1.2883555229029966
1114	17	-1.2209140327060624
1114	18	-2.316854595869991
1114	19	-1.4928611793358257
1114	20	-1.3614988410040354
1114	21	-1.3078687898228836
1114	22	-0.5869876619022278
1114	23	-0.7342265724365618
1114	24	-0.8350182564626323
1114	25	-0.309949160239114
1115	1	-0.1708526207471479
1115	2	0.0685950274914724
1115	3	-0.4124220067662776
1115	4	0.16212872993484595
1115	5	-0.6362726989498029
1115	6	0.347145498401332
1115	7	0.3008423599118615
1115	8	0.2351277986740712
1115	9	-0.9815730967625882
1115	10	-1.4559150200973505
1115	11	-1.6956694066368456
1115	12	-0.5358325150545298
1115	13	0.4467865699094631
1115	14	0.4124827022766482
1115	15	-0.3542110741846187
1115	16	-0.755022189569658
1115	17	0.12273676094473274
1115	18	-0.3605053895207817
1115	19	0.4122975508229061
1115	20	-0.13927661878181397
1115	21	-0.280091012045105
1115	22	-0.2187336936482538
1115	23	0.4717258085158136
1115	24	0.29752142607705423
1115	25	-0.6254253507153038
1116	1	-0.07982697972150632
1116	2	-0.11943916054271497
1116	3	-0.21349038283465305
1116	4	-0.09043537262925838
1116	5	0.14043670275960096
1116	6	0.3103933616491972
1116	7	0.3589620180315194
1116	8	0.2103414738877447
1116	9	-0.2441799343694271
1116	10	-0.19309450727683952
1116	11	0.05924512327768383
1116	12	-0.5330547372767498
1116	13	0.4950771682000621
1116	14	-0.024055759261813137
1116	15	-0.5022879972615399
1116	16	-0.04220167674914421
1116	17	-0.3879469997390288
1116	18	0.05017837116297841
1116	19	-0.8763776628523061
1116	20	-0.6922680717732632
1116	21	-1.1001764821305784
1116	22	-0.6913832662978265
1116	23	-1.1737870119970069
1116	24	-0.0020512234955987196
1116	25	-0.5209381712281242
1201	1	-0.2303764302709581
1201	2	0.13605534495178873
1201	3	0.7760700567257857
1201	4	-0.5640617462556321
1201	5	0.20499714232003896
1201	6	-0.24015608890025675
1201	7	-0.06026875119925279
1201	8	-0.39185632831005535
1201	9	-0.47165246184195553
1201	10	-0.5928197820021168
1201	11	-1.1520186129860548
1201	12	0.3490881198661029
1201	13	-0.28734041421752465
1201	14	0.07319698799093288
1201	15	0.4672174972439507
1201	16	-0.28478409433156315
1201	17	-0.6530568898489193
1201	18	-0.8347117387271297
1201	19	-0.3892897507643909
1201	20	-0.5579274124326048
1201	21	-0.879297361251453
1201	22	0.20586948095491664
1201	23	0.5193448561348646
1201	24	0.02569602925165526
1201	25	0.4936222683323166
1202	1	-0.6053764302709581
1202	2	-0.5960875121910654
1202	3	-0.1525013718456414
1202	4	-1.0819188891127745
1202	5	-1.5628600005371034
1202	6	-1.3472989460431108
1202	7	-1.6674116083421104
1202	8	-1.5347134711672012
1202	9	-2.2752238904133826
1202	10	-1.6106769248592556
1202	11	-0.9198757558431936
1202	12	-1.9723404515624665
1202	13	-1.6801975570746635
1202	14	-0.7482315834376365
1202	15	-0.7470682170417646
1202	16	-0.42764123718870906
1202	17	-1.0101997469917734
1202	18	-1.0668545958699909
1202	19	-1.1392897507643909
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2210	18	1.572034293018902
2210	19	1.378567392092748
2210	20	1.9797710002658064
2210	21	2.1921312101771164
2210	22	1.5828536079390432
2210	23	0.6879956497856554
2210	24	1.634823013378636
2210	25	0.6741778238878737
2211	1	-0.8778418680589759
2211	2	-0.30058059974867035
2211	3	0.046807383914725165
2211	4	0.20782765466602982
2211	5	0.1652505985412347
2211	6	0.22528169911817741
2211	7	0.17360221654268315
2211	8	-0.3849438859137422
2211	9	0.6712046810151904
2211	10	-0.21666770826939796
2211	11	0.3451012026821516
2211	12	1.2540420369168004
2211	13	0.5444568208055216
2211	14	0.6826439925992318
2211	15	0.5207889258153813
2211	16	1.4277735093550703
2211	17	0.6505145387225113
2211	18	0.69236199399176
2211	19	0.9315627837978155
2211	20	0.6385011589959646
2211	21	-0.7917397575648195
2211	22	-0.039522224114207916
2211	23	0.14780107733302117
2211	24	-0.8539122656792202
2211	25	-0.45741459802712825
2212	1	0.6664489665544373
2212	2	0.2590712179676622
2212	3	0.4070224376781688
2212	4	0.5867319045380164
2212	5	0.7546003169232165
2212	6	0.2757169269727626
2212	7	0.11036616943566457
2212	8	1.0049690685153365
2212	9	0.32993483974534854
2212	10	-0.277343591525927
2212	11	0.05631472034728091
2212	12	-0.18067378489580221
2212	13	0.3475802207031151
2212	14	-0.6133109485170039
2212	15	-0.3601634551369983
2212	16	-0.7074031419506142
2212	17	0.5155939038018786
2212	18	0.016478737463344828
2212	19	0.3944404079657673
2212	20	0.21786623836104724
2212	21	0.4460994641453695
2212	22	0.2812663063517462
2212	23	0.6562496180396238
2212	24	0.3808547594103828
2212	25	0.3567175064275574
2213	1	1.3389513008214777
2213	2	1.1192486222627132
2213	3	0.09329694748208794
2213	4	0.4905601024838653
2213	5	1.6619299154292833
2213	6	0.2115245833686572
2213	7	0.4397312488007472
2213	8	0.15016047841263003
2213	9	-0.4170306131024546
2213	10	-0.009836588724802198
2213	11	0.2839057567618468
2213	12	0.2492982038997198
2213	13	0.200054543765674
2213	14	0.09630623168841623
2213	15	0.21196539640361323
2213	16	-0.3824731699618198
2213	17	0.34169100931074325
2213	18	0.18524624446614268
2213	19	0.9752060475549342
2213	20	0.4032070413489066
2213	21	0.8833076807653484
2213	22	0.6260375481818059
2213	23	0.7830003183197363
2213	24	1.259939726730643
2213	25	0.39173151202979994
2214	1	0.8569251570306271
2214	2	0.41780137669782036
2214	3	0.6609906916464219
2214	4	-0.17517285736674282
2214	5	0.008568570891469562
2214	6	0.847145498401332
2214	7	1.475445534515032
2214	8	0.2906833542296283
2214	9	-0.9875254777149678
2214	10	-0.22972454390687602
2214	11	-0.11828845425589662
2214	12	-0.06956267378468795
2214	13	0.6809135540364437
2214	14	0.24383190862585735
2214	15	0.6874555924820456
2214	16	0.24497781043034195
2214	17	-0.039961651753678495
2214	18	-0.06288634190173781
2214	19	1.0293610428864
2214	20	0.6940567145515217
2214	21	0.620702638748547
2214	22	1.3606313857168217
2214	23	1.16418612597613
2214	24	0.17450555306118076
2214	25	0.737669887379937
2215	1	1.8617560749050241
2215	2	1.3777737714528264
2215	3	1.6099209884028056
2215	4	2.2382156864565808
2215	5	1.7580509725477853
2215	6	1.5041503293192093
2215	7	2.2187167560471224
2215	8	2.005659199640256
2215	9	2.330624970870261
2215	10	1.4100270088881572
2215	11	1.7457557141361022
2215	12	2.2727423641725224
2215	13	1.8658686955133277
2215	14	2.0519754558998358
2215	15	1.817890375090741
2215	16	2.201499549560772
2215	17	1.947615987997871
2215	18	2.1117168327014397
2215	19	2.0058965846393306
2215	20	2.442848985082918
2215	21	1.7718413551046552
2215	22	2.315082731472515
2215	23	2.223537402718712
2215	24	1.5595987207630415
2215	25	1.9488500116035397
2216	1	-0.3266497221964251
2216	2	-0.1439653589819585
2216	3	-1.1581949536261789
2216	4	-1.1385959077463141
2216	5	-0.2419490274522147
2216	6	-0.16251633734746207
2216	7	-0.4044716497499792
2216	8	-0.820427756881486
2216	9	-0.24908517405727082
2216	10	0.525969037873665
2216	11	-0.4861283438349133
2216	12	0.678539465621796
2216	13	-1.2355805798489925
2216	14	0.13893197763896836
2216	15	-0.8922545524454861
2216	16	-0.3057468272508217
2216	17	0.6577609155341051
2216	18	-0.5114715730956618
2216	19	-1.1245381979693647
2216	20	-0.9194698554967857
2216	21	-0.5904774854750556
2216	22	-0.8588303120057432
2216	23	-0.29820172771606934
2216	24	0.19727988018333065
2216	25	-0.007671727526897598
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	35.0
3	3.0
4	5.0
5	1.0
6	2.0
7	10.0
8	5.0
9	20.0
10	55.0
11	95.0
12	171.0
13	287.0
14	448.0
15	597.0
16	739.0
17	745.0
18	801.0
19	772.0
20	647.0
21	566.0
22	560.0
23	466.0
24	456.0
25	437.0
26	405.0
27	364.0
28	371.0
29	359.0
30	382.0
31	468.0
32	548.0
33	697.0
34	844.0
35	1075.0
36	2040.0
37	5112.0
38	18473.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.23167921339684	31.433399907819943	11.450811696625186	18.88410918215804
2	15.058202143479821	34.43156761191734	29.526690938926208	20.983539305676633
3	16.467088899715378	40.306161696453756	23.610861816969667	19.615887586861202
4	11.250993564267583	31.64534242711725	27.424937822107125	29.678726186508037
5	11.439928195922553	48.63187588152327	25.405821259135784	14.522374663418386
6	23.400764043791504	47.94502986949722	13.988667538394484	14.665538548316794
7	19.50006409434688	47.37854121266504	17.043968721958723	16.077425971029356
8	20.321993488348244	46.0737816289384	16.28682031430256	17.3174045684108
9	21.207537495192923	31.480579412895782	16.7875913344443	30.524291757466994
10	15.18578352180937	42.52121958099341	23.19922045285535	19.093776444341874
11	24.671862182116488	39.561115668580804	16.255639868744872	19.511382280557836
12	17.858333119696464	42.97177429692107	22.375471069295255	16.794421514087215
13	23.122884398399837	38.05262078161863	20.214893835265155	18.609600984716383
14	18.524216085942108	38.704715021921395	21.170166397456605	21.600902494679897
15	22.51820326120398	40.18818582709466	19.82360783509384	17.470003076607526
16	18.26829393364443	41.4440285113584	21.696323265473566	18.591354289523615
17	17.487949953850887	40.66505999384679	22.461798789867707	19.385191262434624
18	17.30848118141729	38.083273510409185	24.930776330632757	19.677468977540766
19	18.531620907995592	38.216821759081235	25.847881258171192	17.40367607475198
20	20.95897435897436	35.16923076923077	25.51282051282051	18.358974358974358
21	21.919950770493063	35.77087766980333	24.194251429450528	18.114920130253072
22	21.065989847715734	35.661180331231094	24.865405322258113	18.40742449879506
23	20.287142674016152	35.85181387001666	25.21471606204333	18.646327393923855
24	20.469755634759863	35.431677735326545	25.049360240006152	19.049206389907432
25	20.040710107959082	35.78882275643503	25.35879003375332	18.811677101852567
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3281.0
1	3281.0
2	2104.5
3	928.0
4	928.0
5	928.0
6	807.5
7	687.0
8	687.0
9	687.0
10	671.0
11	655.0
12	655.0
13	655.0
14	666.0
15	677.0
16	677.0
17	677.0
18	791.0
19	905.0
20	905.0
21	905.0
22	1197.5
23	1490.0
24	1490.0
25	1490.0
26	1761.0
27	2032.0
28	2032.0
29	2032.0
30	2247.0
31	2462.0
32	2462.0
33	2462.0
34	2647.5
35	2833.0
36	2833.0
37	2833.0
38	2871.0
39	2909.0
40	2909.0
41	2909.0
42	2983.0
43	3057.0
44	3057.0
45	3057.0
46	3672.5
47	4288.0
48	4288.0
49	4288.0
50	4239.5
51	4191.0
52	4191.0
53	4191.0
54	3698.0
55	3205.0
56	3205.0
57	3205.0
58	2779.0
59	2353.0
60	2353.0
61	2353.0
62	2008.0
63	1663.0
64	1663.0
65	1663.0
66	1219.0
67	775.0
68	775.0
69	775.0
70	579.0
71	383.0
72	383.0
73	383.0
74	282.0
75	181.0
76	181.0
77	181.0
78	123.5
79	66.0
80	66.0
81	66.0
82	47.5
83	29.0
84	29.0
85	29.0
86	18.5
87	8.0
88	8.0
89	8.0
90	4.5
91	1.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01792068815442513
2	0.1510458001587261
3	0.15872609508205116
4	0.1536058984665011
5	0.16896648831315123
6	0.14848570185095109
7	0.14336550523540104
8	0.138245308619851
9	0.14336550523540104
10	0.16384629169760118
11	0.13568521031207598
12	0.138245308619851
13	0.16640639000537621
14	0.14848570185095109
15	0.14592560354317605
16	0.1510458001587261
17	0.14592560354317605
18	0.14592560354317605
19	0.13312511200430097
20	0.15616599677427614
21	0.1536058984665011
22	0.14080540692762603
23	0.14336550523540104
24	0.15872609508205116
25	0.6400245769437546
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	39061.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.96362100304652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.3914248519716	50.83843219579632
2	10.351488682652333	12.621284657330841
3	2.9395708226598916	5.376206446327539
4	1.217822197959098	2.9697140370190214
5	0.4955276529626675	1.510458001587261
6	0.3401503380506446	1.244207777578659
7	0.18477302313862176	0.7885102787947058
8	0.15957670180153696	0.7782698855636057
9	0.11338344601688154	0.6221038887893295
>10	0.6970982236593457	7.506208238396354
>50	0.06299080334271197	2.2708071989964416
>100	0.041993868895141305	5.396687232789739
>500	0.0	0.0
>1k	0.004199386889514131	8.077110161030184
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	3155	8.077110161030184	No Hit
GTATCAACGCAGAGTACTTTTTTTT	423	1.0829215841888329	No Hit
GGTATCAACGCAGAGTACTTTTTTT	409	1.0470802078799826	No Hit
TATCAACGCAGAGTACTTTTTTTTT	261	0.6681856583292799	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	258	0.6605053634059548	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	154	0.3942551393973529	No Hit
GTACATGGGAAGCAGTGGTATCAAC	140	0.3584137630885026	No Hit
GTGGTATCAACGCAGAGTACATGGG	126	0.32257238677965233	No Hit
CCCATGTACTCTGCGTTGATACCAC	123	0.31489209185632727	No Hit
AAAAACAAAAAAAAAAAAAAAAAAA	108	0.276490617239702	No Hit
ACACAAAAAAAAAAAAAAAAAAAAA	106	0.27137042062415195	No Hit
GTGGTATCAACGCAGAGTACTTTTT	74	0.18944727477535137	No Hit
GGTATCAACGCAGAGTACATGGGGG	73	0.18688717646757635	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	68	0.17408668492870127	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	65	0.16640639000537621	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	64	0.16384629169760118	No Hit
GAGTACATGGGAAGCAGTGGTATCA	59	0.1510458001587261	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA	58	0.14848570185095109	No Hit
GGTATCAACGCAGAGTACATGGGAA	57	0.14592560354317605	No Hit
GTACTGGTTCACTATCGGTCAGTCA	55	0.14080540692762603	No Hit
GGTATCAACGCAGAGTACATGGGGA	54	0.138245308619851	No Hit
GGTATCAACGCAGAGTACATGGGCA	54	0.138245308619851	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	53	0.13568521031207598	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA	51	0.13056501369652596	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	51	0.13056501369652596	No Hit
ATACAGGGTGACAGCCCCGTACACA	51	0.13056501369652596	No Hit
TATCAACGCAGAGTACATGGGAAGC	50	0.12800491538875092	No Hit
GGATACCACGTGTCCCGCCCTACTC	45	0.11520442384987584	No Hit
CATGTACTCTGCGTTGATACCACTG	42	0.10752412892655078	No Hit
AAAAAAAACAAAAAAAAAAAAAAAA	41	0.10496403061877575	No Hit
GTATCAACGCAGAGTACATGGGAAG	40	0.10240393231100076	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	40	0.10240393231100076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAAAAA	85	6.295075E-4	10.058144	1
GGTATCA	155	3.7718564E-7	9.192927	1
GTATCAA	210	2.857516E-5	6.785256	2
TATCAAC	240	1.8119317E-4	5.9370985	3
TACATGG	275	2.9563009E-5	5.87233	2
GTACATG	285	5.1035495E-5	5.666284	1
ACATGGG	290	6.649432E-5	5.568589	3
AACGCAG	260	5.3775916E-4	5.4803987	7
ATCAACG	265	6.950553E-4	5.376995	4
TCAACGC	270	8.9326536E-4	5.277421	5
ACAAAAA	310	1.8196156E-4	5.2093253	3
CAACGCA	275	0.0011616014	5.174825	6
>>END_MODULE