FastQCFastQC Report
Wed 25 May 2016
SRR1294816_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294816_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39061
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17474.472491743682958No Hit
GGTATCAACGCAGAGTACTTTTTTT16804.300965157062031No Hit
TATCAACGCAGAGTACTTTTTTTTT12543.210363277949873No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7671.963595402063439No Hit
GTACTTTTTTTTTTTTTTTTTTTTT4991.2774890555797342No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4211.0778013875732828No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3951.0112388315711323No Hit
GTGGTATCAACGCAGAGTACTTTTT2930.7501088041780803No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2910.7449886075625304No Hit
ATCAACGCAGAGTACTTTTTTTTTT1640.419856122475103No Hit
CCCATGTACTCTGCGTTGATACCAC1470.37633445124292775No Hit
GTACATGGGAAGCAGTGGTATCAAC1330.3404930749340775No Hit
GTGGTATCAACGCAGAGTACATGGG1270.32513248508742737No Hit
AACGCAGAGTACTTTTTTTTTTTTT1050.2688103223163769No Hit
CAACGCAGAGTACTTTTTTTTTTTT1040.26625022400860193No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT830.21248815954532657No Hit
GGTATCAACGCAGAGTACATGGGGG820.2099280612375515No Hit
GGTATCAACGCAGAGTACATGGGAA750.1920073730831264No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT730.18688717646757635No Hit
TCAACGCAGAGTACTTTTTTTTTTT650.16640639000537621No Hit
GAGTACATGGGAAGCAGTGGTATCA610.15616599677427614No Hit
GTACTGGTTCACTATCGGTCAGTCA600.1536058984665011No Hit
CATGTACTCTGCGTTGATACCACTG590.1510458001587261No Hit
GCGTTGATACCACTGCTTCCCATGT560.14336550523540104No Hit
GGTATCAACGCAGAGTACATGGGGA530.13568521031207598No Hit
ACGCAGAGTACATGGGAAGCAGTGG530.13568521031207598No Hit
GGATACCACGTGTCCCGCCCTACTC510.13056501369652596No Hit
TATCAACGCAGAGTACATGGGAAGC470.12032462046542586No Hit
GGTATCAACGCAGAGTACATGGGCA470.12032462046542586No Hit
ACTCTGCGTTGATACCACTGCTTCC450.11520442384987584No Hit
GTATCAACGCAGAGTACATGGGAAG430.1100842272343258No Hit
GGTATCAACGCAGAGTACATGGGGT410.10496403061877575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3350.012.5590661
GTGGTAT1053.9777966E-49.1066821
TGGTATC1200.00150105837.9683462
GTATCAA5500.07.64961242
TCAACGC7200.05.97625975
TATCAAC7050.05.9677833
CAACGCA7350.05.9383576
ATCAACG7250.05.93504434
AACGCAG7350.05.9307537
AGTACTT6901.8189894E-125.21883913
GAGTACT6901.8189894E-125.21883912
ACGCAGA8450.05.1587038
CGCAGAG8300.05.13775979
Produced by FastQC (version 0.11.5)