##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294816_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 39061 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.888277309848696 33.0 31.0 34.0 30.0 34.0 2 31.893781521210414 33.0 31.0 34.0 30.0 34.0 3 31.953739023578507 34.0 31.0 34.0 28.0 34.0 4 35.37548961880136 37.0 35.0 37.0 33.0 37.0 5 35.53744143775121 37.0 35.0 37.0 33.0 37.0 6 35.32697575586903 37.0 36.0 37.0 33.0 37.0 7 35.33434883899542 37.0 36.0 37.0 33.0 37.0 8 35.33921302578019 37.0 37.0 37.0 33.0 37.0 9 36.97260694810681 39.0 39.0 39.0 34.0 39.0 10 37.082025549781115 39.0 39.0 39.0 34.0 39.0 11 37.078902229845625 39.0 39.0 39.0 34.0 39.0 12 37.09830777501856 39.0 39.0 39.0 34.0 39.0 13 36.987122705511894 39.0 38.0 39.0 34.0 39.0 14 38.25096643711119 41.0 39.0 41.0 33.0 41.0 15 38.19333862420317 41.0 39.0 41.0 33.0 41.0 16 38.18335424080285 41.0 38.0 41.0 33.0 41.0 17 38.37600163846292 41.0 39.0 41.0 34.0 41.0 18 38.4028314687284 41.0 39.0 41.0 34.0 41.0 19 38.50037121425463 41.0 39.0 41.0 34.0 41.0 20 38.50346893320704 41.0 39.0 41.0 34.0 41.0 21 38.488799569903485 41.0 39.0 41.0 34.0 41.0 22 38.469854842425946 41.0 39.0 41.0 34.0 41.0 23 38.40311307954225 41.0 39.0 41.0 33.0 41.0 24 38.36617086096106 41.0 39.0 41.0 33.0 41.0 25 37.52036558203835 40.0 37.0 41.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.6588427167359612 1101 2 -0.9849570959242513 1101 3 -0.2799090547198908 1101 4 -0.22339876749710186 1101 5 0.3128706365301568 1101 6 -0.05010346772191099 1101 7 0.11310084008359667 1101 8 -0.11217872675728557 1101 9 0.3246449815944672 1101 10 0.4094326992654018 1101 11 -0.19206304158940668 1101 12 0.055504525794674464 1101 13 0.033668274657031816 1101 14 0.07337508050177632 1101 15 0.44886490294287995 1101 16 0.37938293785500576 1101 17 0.3042068606476178 1101 18 0.382706812532561 1101 19 0.08200616639854275 1101 20 -0.036810439125822825 1101 21 0.12107443416269348 1101 22 0.20538631374584781 1101 23 0.45482050187017364 1101 24 0.46236089660184376 1101 25 0.30835054506025017 1102 1 -0.671157987671922 1102 2 -0.7768290171065182 1102 3 0.2428741965116359 1102 4 -1.05837413695523 1102 5 -0.22900128465211367 1102 6 -0.042098541613540874 1102 7 -0.23111098257649587 1102 8 -0.5155038499099902 1102 9 -0.6501087129868139 1102 10 0.1569696450782132 1102 11 0.10658227860763958 1102 12 0.04072620067152144 1102 13 -0.450937636673018 1102 14 0.3855671987283813 1102 15 0.25674667634189774 1102 16 -0.2800998207656846 1102 17 -0.49443845954942844 1102 18 0.21214031006950762 1102 19 -0.07562930158174908 1102 20 -0.13163802533271962 1102 21 -0.28779256091119265 1102 22 -1.0421506320669636 1102 23 -0.5538001877850007 1102 24 -0.4582056058612096 1102 25 0.06943428890261316 1103 1 -0.16016897668291108 1103 2 0.07207208179458036 1103 3 -0.013993935356495513 1103 4 0.5212961927150985 1103 5 0.7160536604028351 1103 6 0.975758601243605 1103 7 0.924108797643278 1103 8 0.9693862599801193 1103 9 0.9639022760241787 1103 10 1.209167447276016 1103 11 1.5804833775087417 1103 12 0.8539130138583388 1103 13 1.506479945744566 1103 14 1.3896880778492573 1103 15 1.1358675554627737 1103 16 0.9218232561573956 1103 17 1.0316604415494695 1103 18 1.6393381122673105 1103 19 1.1304146544622071 1103 20 1.1183619746672804 1103 21 0.8578118346932015 1103 22 1.0210361811198538 1103 23 1.0038921199073059 1103 24 0.8343768117212065 1103 25 0.9018518713201971 1104 1 -1.153637772038497 1104 2 -0.8691470764057101 1104 3 -0.4272740514667994 1104 4 -0.41956012078271954 1104 5 -1.7599985892343213 1104 6 -1.0855621534733757 1104 7 -1.3621756726034562 1104 8 -0.47001867470783765 1104 9 -0.10866666446928974 1104 10 -0.2668848023611403 1104 11 -1.3937546486160812 1104 12 -0.08730614434195161 1104 13 0.02716209378520773 1104 14 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2214 18 -0.4366692137400179 2214 19 -0.42483565078809704 2214 20 -0.14354278723747882 2214 21 -0.5576338307524651 2214 22 -0.36357920349553297 2214 23 -0.49427637826119053 2214 24 -0.5157452884008933 2214 25 -0.5813593618910389 2215 1 0.7367095071520922 2215 2 0.9700653928313692 2215 3 0.9662178818118434 2215 4 1.2501144758812117 2215 5 0.9780380528220078 2215 6 1.2330049780551988 2215 7 1.2727710050011396 2215 8 0.651939214272204 2215 9 0.7119513284210583 2215 10 0.8769489411444624 2215 11 0.20673755811074557 2215 12 1.1287179190980225 2215 13 0.40698161798536603 2215 14 0.57112620493956 2215 15 0.2774506100893106 2215 16 0.859392932857503 2215 17 0.390913507324278 2215 18 0.8324819249763351 2215 19 0.37985724086131256 2215 20 0.7343040036527881 2215 21 0.6418140643476065 2215 22 0.4914932602725841 2215 23 5.47638301952702E-4 2215 24 0.8251794873064924 2215 25 0.16467238414070806 2216 1 0.22946313034049837 2216 2 0.40484800152702505 2216 3 0.45897150500024964 2216 4 0.4240275193594698 2216 5 0.6012264586191094 2216 6 0.9141643983450578 2216 7 0.5336405702185303 2216 8 0.46353341717075125 2216 9 0.8134006037833785 2216 10 0.7175286512893919 2216 11 0.6705056740527766 2216 12 0.8678483538806319 2216 13 0.16060480639116292 2216 14 0.6870682339250678 2216 15 0.6252766970458268 2216 16 1.0043204690893859 2216 17 0.8256961160199268 2216 18 0.6440761278748894 2216 19 0.3363789799917498 2216 20 0.06763733698612384 2216 21 0.17804594840557542 2216 22 0.23062369505519342 2216 23 0.20344618902659306 2216 24 0.549817168465907 2216 25 0.12119412327114532 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 6.0 9 13.0 10 29.0 11 36.0 12 47.0 13 61.0 14 87.0 15 109.0 16 147.0 17 170.0 18 192.0 19 212.0 20 212.0 21 212.0 22 190.0 23 148.0 24 182.0 25 144.0 26 165.0 27 165.0 28 151.0 29 157.0 30 200.0 31 261.0 32 399.0 33 492.0 34 785.0 35 1355.0 36 2213.0 37 5604.0 38 24916.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.604691060866 21.25316877064502 15.153765395744244 17.98837477274473 2 17.4829113437956 24.392616676480376 36.12298712270551 22.00148485701851 3 19.27242006093034 30.273162489439592 31.386805253321725 19.06761219630834 4 12.055502931312564 21.22833516807045 34.15427152402653 32.56189037659046 5 11.384496721943695 38.52166088186142 32.74971075973775 17.344131636457128 6 26.5720652647217 38.2444097231116 16.682462027099714 18.501062985066984 7 20.432199918066367 39.45616550594019 21.768742318721834 18.342892257271608 8 25.155525972197335 33.483525767389466 20.411663807890225 20.949284452522974 9 25.00448017203861 16.571516346227693 22.25237449118046 36.17162899055324 10 19.367143698318014 30.9490284426922 27.597859757814703 22.085968101175084 11 28.49645426384373 26.70950564501677 20.304139678963672 24.489900412175828 12 23.11512762090064 31.681216558715853 28.59885819615473 16.60479762422877 13 29.594736437879217 25.26561019943166 26.67366426870792 18.465989093981207 14 22.44182176595581 26.47653669900924 29.935229512813294 21.146412022221654 15 26.461176109162594 26.973195770717595 28.790865569237862 17.774762550881952 16 18.01797189012058 30.24756150636184 31.299761910857377 20.434704692660198 17 17.72657450076805 29.034818228366614 31.118791602662572 22.119815668202765 18 17.569954686259955 24.054683699854074 36.94221858119352 21.433143032692456 19 18.38662604644018 24.968638795729756 40.244745398223294 16.39998975960677 20 20.09933181434167 21.561147948081206 40.436752771306416 17.902767466270706 21 20.862775217613926 22.688172043010752 39.49308755760369 16.955965181771635 22 19.548910678170042 22.88471877320089 40.283146872839914 17.28322367578915 23 18.931926985996263 22.70551189165664 40.70044289700724 17.662118225339853 24 19.0173839576026 22.68875291226094 40.53099157684529 17.762871553291173 25 18.748398995850195 22.744505353757877 40.463138480454944 18.043957169936984 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 515.0 1 515.0 2 428.0 3 341.0 4 341.0 5 341.0 6 508.0 7 675.0 8 675.0 9 675.0 10 635.0 11 595.0 12 595.0 13 595.0 14 485.0 15 375.0 16 375.0 17 375.0 18 602.0 19 829.0 20 829.0 21 829.0 22 1301.0 23 1773.0 24 1773.0 25 1773.0 26 2354.5 27 2936.0 28 2936.0 29 2936.0 30 3208.0 31 3480.0 32 3480.0 33 3480.0 34 3730.0 35 3980.0 36 3980.0 37 3980.0 38 3586.0 39 3192.0 40 3192.0 41 3192.0 42 3130.0 43 3068.0 44 3068.0 45 3068.0 46 3663.5 47 4259.0 48 4259.0 49 4259.0 50 4271.0 51 4283.0 52 4283.0 53 4283.0 54 3736.0 55 3189.0 56 3189.0 57 3189.0 58 2780.0 59 2371.0 60 2371.0 61 2371.0 62 2031.5 63 1692.0 64 1692.0 65 1692.0 66 1257.5 67 823.0 68 823.0 69 823.0 70 631.0 71 439.0 72 439.0 73 439.0 74 303.0 75 167.0 76 167.0 77 167.0 78 110.0 79 53.0 80 53.0 81 53.0 82 35.5 83 18.0 84 18.0 85 18.0 86 10.5 87 3.0 88 3.0 89 3.0 90 3.0 91 3.0 92 3.0 93 3.0 94 2.0 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020480786462200147 2 0.0 3 0.0 4 0.0 5 0.42497631909065314 6 0.05120196615550038 7 0.012800491538875094 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0025600983077750184 18 0.0 19 0.0 20 0.0 21 0.0025600983077750184 22 0.0 23 0.0 24 0.005120196615550037 25 0.05888226107882543 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 39061.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.376488057141394 #Duplication Level Percentage of deduplicated Percentage of total 1 81.72787193973635 44.440746524666544 2 10.965160075329566 11.924937917616036 3 3.6346516007532954 5.929187680806943 4 1.3370998116760828 2.9082716776324213 5 0.6026365348399246 1.638462916976012 6 0.38135593220338987 1.244207777578659 7 0.20244821092278717 0.7705895906402807 8 0.17890772128060262 0.7782698855636057 9 0.1318267419962335 0.6451447735593047 >10 0.7109227871939736 6.9404265123780755 >50 0.05649717514124294 1.9738357952945393 >100 0.051789077212806026 6.858503366529274 >500 0.004708097928436911 1.963595402063439 >1k 0.014124293785310734 11.983820178694863 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1747 4.472491743682958 No Hit GGTATCAACGCAGAGTACTTTTTTT 1680 4.300965157062031 No Hit TATCAACGCAGAGTACTTTTTTTTT 1254 3.210363277949873 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 767 1.963595402063439 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 499 1.2774890555797342 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 421 1.0778013875732828 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 395 1.0112388315711323 No Hit GTGGTATCAACGCAGAGTACTTTTT 293 0.7501088041780803 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 291 0.7449886075625304 No Hit ATCAACGCAGAGTACTTTTTTTTTT 164 0.419856122475103 No Hit CCCATGTACTCTGCGTTGATACCAC 147 0.37633445124292775 No Hit GTACATGGGAAGCAGTGGTATCAAC 133 0.3404930749340775 No Hit GTGGTATCAACGCAGAGTACATGGG 127 0.32513248508742737 No Hit AACGCAGAGTACTTTTTTTTTTTTT 105 0.2688103223163769 No Hit CAACGCAGAGTACTTTTTTTTTTTT 104 0.26625022400860193 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 83 0.21248815954532657 No Hit GGTATCAACGCAGAGTACATGGGGG 82 0.2099280612375515 No Hit GGTATCAACGCAGAGTACATGGGAA 75 0.1920073730831264 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 73 0.18688717646757635 No Hit TCAACGCAGAGTACTTTTTTTTTTT 65 0.16640639000537621 No Hit GAGTACATGGGAAGCAGTGGTATCA 61 0.15616599677427614 No Hit GTACTGGTTCACTATCGGTCAGTCA 60 0.1536058984665011 No Hit CATGTACTCTGCGTTGATACCACTG 59 0.1510458001587261 No Hit GCGTTGATACCACTGCTTCCCATGT 56 0.14336550523540104 No Hit GGTATCAACGCAGAGTACATGGGGA 53 0.13568521031207598 No Hit ACGCAGAGTACATGGGAAGCAGTGG 53 0.13568521031207598 No Hit GGATACCACGTGTCCCGCCCTACTC 51 0.13056501369652596 No Hit TATCAACGCAGAGTACATGGGAAGC 47 0.12032462046542586 No Hit GGTATCAACGCAGAGTACATGGGCA 47 0.12032462046542586 No Hit ACTCTGCGTTGATACCACTGCTTCC 45 0.11520442384987584 No Hit GTATCAACGCAGAGTACATGGGAAG 43 0.1100842272343258 No Hit GGTATCAACGCAGAGTACATGGGGT 41 0.10496403061877575 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 335 0.0 12.559066 1 GTGGTAT 105 3.9777966E-4 9.106682 1 TGGTATC 120 0.0015010583 7.968346 2 GTATCAA 550 0.0 7.6496124 2 TCAACGC 720 0.0 5.9762597 5 TATCAAC 705 0.0 5.967783 3 CAACGCA 735 0.0 5.938357 6 ATCAACG 725 0.0 5.9350443 4 AACGCAG 735 0.0 5.930753 7 AGTACTT 690 1.8189894E-12 5.218839 13 GAGTACT 690 1.8189894E-12 5.218839 12 ACGCAGA 845 0.0 5.158703 8 CGCAGAG 830 0.0 5.1377597 9 >>END_MODULE