FastQCFastQC Report
Wed 25 May 2016
SRR1294815_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294815_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2847187
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC158770.5576381179037415No Hit
GTACATGGGAAGCAGTGGTATCAAC157100.5517726794903179No Hit
GAGTACATGGGAAGCAGTGGTATCA72830.2557963351195408No Hit
CATGTACTCTGCGTTGATACCACTG66850.2347931484654854No Hit
GCGTTGATACCACTGCTTCCCATGT59060.20743280999807884No Hit
GTATCAACGCAGAGTACATGGGAAG52440.18418179065863957No Hit
ACGCAGAGTACATGGGAAGCAGTGG40070.14073539953645475No Hit
ACTCTGCGTTGATACCACTGCTTCC38870.13652071325136003No Hit
ATTCCATTCCATTCCATTCCATTCC36100.12679181240993304No Hit
TATCAACGCAGAGTACATGGGAAGC34070.11966196811098112No Hit
GGTATCAACGCAGAGTACATGGGAA31720.11140820746933727No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCC700.001495766610.85391411
CTAGTAC1252.240522E-710.6405813
GTCTAAC1701.382432E-1010.6189761
TATTCCG909.5169424E-510.5559475
TAGGACT3400.010.3393474
CTAGGAC2950.010.3056483
CTATACT3250.010.2318694
GTGTAGG3150.09.9536261
TCTATAC2700.09.852393
CTACACT4450.09.821284
ATTAGAC1651.02227204E-89.7884143
GTATAGG2900.09.5011881
AAACCCG1201.7006656E-59.5003525
TACACTG5750.09.2525175
GCACTGT4750.09.1958086
GTATAAG3100.09.1946981
GTATTAG4400.09.0693161
CTATACC3250.09.0625124
TTCTATA3250.09.0621932
AAGACCG950.00182381079.0003345
Produced by FastQC (version 0.11.5)