FastQCFastQC Report
Wed 25 May 2016
SRR1294815_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294815_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2847187
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC159780.5611854788603629No Hit
GTACATGGGAAGCAGTGGTATCAAC156700.5503677840619531No Hit
GAGTACATGGGAAGCAGTGGTATCA78860.27697513370214183No Hit
CATGTACTCTGCGTTGATACCACTG66130.23226433669442859No Hit
GTATCAACGCAGAGTACTTTTTTTT63730.22383496412423914No Hit
GCGTTGATACCACTGCTTCCCATGT58610.20585230264116827No Hit
GTATCAACGCAGAGTACATGGGAAG55540.19506973022846763No Hit
ACGCAGAGTACATGGGAAGCAGTGG42850.15049942276359088No Hit
TATCAACGCAGAGTACATGGGAAGC37690.13237627173768354No Hit
GGTATCAACGCAGAGTACTTTTTTT36880.1295313584952446No Hit
ACTCTGCGTTGATACCACTGCTTCC36810.12928550179528076No Hit
ATTCCATTCCATTCCATTCCATTCC32530.11425312071177622No Hit
GGTATCAACGCAGAGTACATGGGAA32400.11379652969755762No Hit
TATCAACGCAGAGTACTTTTTTTTT31450.11045990305519097No Hit
GAATGGAATGGAATGGAATGGAATG29250.10273297819918395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACCG951.002134E-612.0376415
GTCTAGC1303.67314E-710.2648531
TATACTG4250.010.0903755
ATACCGT856.64913E-410.0501376
TAGACAG4400.09.7463865
TTAGACA2700.09.5297994
AGACCGT1201.7189004E-59.4917966
TGTCCGA800.0045622429.48862910
TTACACT4000.09.29155354
TTATACT3600.09.2650824
CTAATAC2700.09.1768443
GTCTAGA3350.09.1048571
CTGTCGC2850.08.9892279
CACACCG850.00724926648.9692225
TAGGACC1606.447117E-78.9341874
GTCCTAG3850.08.912711
TTAGGAC2159.822543E-108.8649283
GTTCTAC1401.0409965E-58.8508161
GTCTAGG2800.08.8508161
GTGTAAC2053.9035513E-98.834211
Produced by FastQC (version 0.11.5)