FastQCFastQC Report
Wed 25 May 2016
SRR1294814_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294814_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816839
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC114990.6329124374807014No Hit
CCCATGTACTCTGCGTTGATACCAC114030.6276285350545646No Hit
GAGTACATGGGAAGCAGTGGTATCA56090.30872300737709835No Hit
CATGTACTCTGCGTTGATACCACTG48620.2676076416237212No Hit
GCGTTGATACCACTGCTTCCCATGT38970.21449341411099165No Hit
GTATCAACGCAGAGTACATGGGAAG31910.17563471501877712No Hit
ACGCAGAGTACATGGGAAGCAGTGG28970.15945276383873308No Hit
ACTCTGCGTTGATACCACTGCTTCC24590.13534495901948385No Hit
GCTTCCCATGTACTCTGCGTTGATA20480.11272325175758556No Hit
TATCAACGCAGAGTACATGGGAAGC18840.10369658511293516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCG307.7168416E-419.0024015
ATACCGT350.002177366316.277896
CGGGTCT851.027729E-915.639546
TACCGTT400.005288663314.2462967
AACGCGA400.005289631414.24590311
CGACCGC655.465366E-513.15078910
TTATACT957.36909E-813.002724
GTCGCGC604.1025458E-412.6630268
TTAGACT853.93512E-612.2966894
CACGGGT1302.6066118E-911.6947534
TACGACC600.00586715311.0856524
TTACGGA600.00586715311.0856524
CGTACCC700.00149003810.8585153
TTACACT2001.8189894E-1210.4521864
GTGTTAG2100.010.4069381
CTACACT3400.010.3403984
TAGAACT1201.5196656E-610.2938194
TATACTG1303.834757E-710.2320615
TACACCG750.0026451610.1346145
TACGTCG750.0026451610.1346145
Produced by FastQC (version 0.11.5)