FastQCFastQC Report
Wed 25 May 2016
SRR1294814_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294814_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816839
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC115630.6364350390981259No Hit
GTACATGGGAAGCAGTGGTATCAAC111810.6154095106941231No Hit
GAGTACATGGGAAGCAGTGGTATCA55040.30294373909851124No Hit
CATGTACTCTGCGTTGATACCACTG50350.2771296741208219No Hit
GCGTTGATACCACTGCTTCCCATGT40850.22484105636217627No Hit
GTATCAACGCAGAGTACATGGGAAG32240.17745105647776166No Hit
ACGCAGAGTACATGGGAAGCAGTGG30320.166883251625488No Hit
ACTCTGCGTTGATACCACTGCTTCC25110.13820707283364128No Hit
GCTTCCCATGTACTCTGCGTTGATA20460.11261317045704106No Hit
GTATCAACGCAGAGTACTTTTTTTT20120.11074178834778427No Hit
TATCAACGCAGAGTACATGGGAAGC19670.10826495908553263No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAT250.00590729619.069091
AGTACGG250.005914850619.064875
GCGCGAA350.00218944716.26441211
GTACGAC902.1809683E-914.8282343
GGTACGA851.6394551E-814.5806312
TACGACC1051.3023964E-913.6177654
ATGTCGC905.458405E-712.6567176
CGACCAT1155.3878466E-912.381576
TATGTCG701.05691994E-412.2559895
GGTACCG550.002991429312.1321915
TGGCGTA1353.5106495E-1012.0038085
GGCGTAC1603.6379788E-1211.8656726
CTAGACT1459.094947E-1111.8333684
GACTGCG658.107537E-411.6779787
ATTAGAC907.184499E-611.6507553
TTGCGGA1657.2759576E-1211.50578919
CTTATAC1104.755093E-711.2656053
TTGGCGT1552.9467628E-1011.0699254
CTTTGCG1803.6379788E-1211.06883617
GGGTACG1053.319099E-610.8966231
Produced by FastQC (version 0.11.5)