FastQCFastQC Report
Wed 25 May 2016
SRR1294813_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294813_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846183
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC22430.12149391474192969No Hit
CCCATGTACTCTGCGTTGATACCAC20790.11261072168901999No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGC350.002175237516.2802936
GTCCGGA500.001501039813.298461
CCCCGCG604.103235E-412.66279412
TGCACCG1052.7171336E-711.7630995
TGCTAGC1354.7421054E-911.2573442
CGTCTAT1201.2852797E-711.07723815
TCGTCTA1201.2852797E-711.07723814
GTATTAC1553.1286618E-1011.0309811
CTCGTCT1408.392817E-910.85382413
GCACCGT1752.0008883E-1110.8535286
CTTGCGT1053.4896675E-610.85117215
GTCTAGT1158.831139E-710.7378861
CACCGTG2950.010.6264667
GTCTTAT909.527525E-510.5543341
GCTCGTC1451.45028025E-810.47955312
ATGCGGG1106.0543716E-610.3624371
GTGCTAG1856.002665E-1110.2690821
CGGAAGT750.002645144210.1346374
ACCGGAC750.002651707310.131613
CGTGCGT856.5995485E-410.05793310
Produced by FastQC (version 0.11.5)