FastQCFastQC Report
Wed 25 May 2016
SRR1294813_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294813_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846183
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35980.19488858905103124No Hit
CCCATGTACTCTGCGTTGATACCAC22870.12387721043905181No Hit
GGTATCAACGCAGAGTACTTTTTTT22560.12219807028880669No Hit
GTACATGGGAAGCAGTGGTATCAAC21330.11553567549912441No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG551.9059959E-413.8641525
TCGTCCG707.3620267E-613.5538829
TCGTCTA951.0571821E-611.98383514
TCCTATA1302.4792826E-911.7324822
GTTCCCG600.005716612611.1244431
TATACCG600.005732852511.1202065
TACCGCC600.00593202911.0693037
GCCGACA600.00593322511.06900218
TCTCACG1553.1650416E-1011.02545919
TGCGGGA1303.1057425E-810.9992022
TTAGGCA1252.1401502E-710.6753974
CGTCCGC909.642009E-510.54190710
CTAGACT1451.3706085E-810.51763254
CGTCTAT1106.144064E-610.34967615
GTTTAAC1502.3081157E-810.170921
GCTCGTC1409.881478E-810.16486112
TAGACCA1803.7107384E-1010.0611374
GTAGCAC1803.7107384E-1010.0611373
GTATTAG2002.3646862E-1110.0119991
GTCTAGA1053.9416504E-59.9892951
Produced by FastQC (version 0.11.5)