FastQCFastQC Report
Wed 25 May 2016
SRR1294812_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294812_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1715502
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC46790.272748151852927No Hit
CCCATGTACTCTGCGTTGATACCAC41420.24144536118290738No Hit
GAGTACATGGGAAGCAGTGGTATCA24340.14188266758068485No Hit
ATTCCATTCCATTCCATTCCATTCC19950.11629249047800584No Hit
CATGTACTCTGCGTTGATACCACTG19030.11092962876172688No Hit
GTATCAACGCAGAGTACATGGGAAG18940.11040500098513438No Hit
GCGTTGATACCACTGCTTCCCATGT18900.1101718330844266No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGGG456.77735E-414.77359612
TACGACC400.00527292914.2526314
AATTTCG400.005298550214.24222215
AAATGCG550.003065764212.0924365
ATTTCGT550.003081910812.08395816
TATATCG600.005870553711.0847315
AATGCGT600.005899998411.0772836
GCCTAAG700.001489602710.858831
GTCTTCG700.001494696610.8543877
GTTCTAG1502.1263986E-910.7683391
GTTATAC1002.3954242E-510.4513183
GTCCTAA1502.4381734E-810.1349081
AACCGTG750.002653385710.1307617
ACACGGT1502.4649125E-810.1278026
TATACAG2600.09.8666295
GTATTAG1951.6370905E-109.7451041
TTAGAGT1502.6402267E-79.5017554
TAGTACT2500.09.5017554
ATGCGGG800.0045100589.5014761
CCTCTAG1304.2293086E-69.5014751
Produced by FastQC (version 0.11.5)