FastQCFastQC Report
Wed 25 May 2016
SRR1294812_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294812_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1715502
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48110.2804426925762838No Hit
GTACATGGGAAGCAGTGGTATCAAC47550.27717834196637486No Hit
CCCATGTACTCTGCGTTGATACCAC44860.2614978006437766No Hit
GGTATCAACGCAGAGTACTTTTTTT31920.18606798476480937No Hit
GAGTACATGGGAAGCAGTGGTATCA25960.15132596755935No Hit
TATCAACGCAGAGTACTTTTTTTTT24460.14258217128280817No Hit
CATGTACTCTGCGTTGATACCACTG20380.11879904541061449No Hit
ATTCCATTCCATTCCATTCCATTCC19970.11640907442835974No Hit
GTATCAACGCAGAGTACATGGGAAG18680.1088894096305338No Hit
GCGTTGATACCACTGCTTCCCATGT18350.1069657744496946No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC250.006080114318.9740610
GTCGTGC500.001515175713.2818418
TAACACG600.005728136711.1213614
GGAACGA1002.4344234E-510.43603715
TCTCACG1201.5383775E-610.2839119
CTAGCAC1303.664445E-710.2658713
ATTAGGC1409.213363E-810.2134943
TCAGCAC3650.010.18551353
ACGGTGT750.002677987310.1194988
GTCCTAA1356.1048377E-79.8905781
CCTATAC1856.0936145E-109.7902333
GGACCAT1752.6047928E-99.7640646
GCCTTAT2002.4556357E-109.5373441
GTCTACT1106.548535E-59.5373441
GTCCCGC800.0043720149.5373431
TCTAGTA1106.571671E-59.534272
AATCCTG1502.5188092E-79.5325955
TAGAACC900.00107928249.5325954
CAGGGGT2600.09.5325954
TCTACAC2201.6370905E-119.5325953
Produced by FastQC (version 0.11.5)