FastQCFastQC Report
Wed 25 May 2016
SRR1294811_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294811_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132577
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC41690.19549118273337845No Hit
CCCATGTACTCTGCGTTGATACCAC39670.18601907457503294No Hit
ATTCCATTCCATTCCATTCCATTCC24250.11371218952469242No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCCG551.9646414E-413.8148999
CGTCCGC751.4828074E-512.66365710
GTCCGCA951.0433487E-611.99714911
GTCTCGT700.00149475310.8545649
ATCGTCC700.00149475310.8545648
GACTGCG700.00149475310.8545647
TCTCGTG803.780194E-410.68496210
ACCGTCT909.540886E-510.5530498
TGGACAG3900.09.9892675
TAGGCTA1054.0967163E-59.9544635
GGTTTCG900.00111629099.4977448
GCTCGTT900.00111629099.49774411
TAATACT3650.09.3720434
GTACAAG1554.245212E-79.1959041
GTATAAT3100.09.1959041
CGGGTTC1356.8977097E-69.14296718
TAAACTG3650.09.1114945
TCGTGGG1151.1136771E-49.08479912
GTCTAGA1804.20132E-88.9745221
GTCTTAC2553.6379788E-128.9434671
Produced by FastQC (version 0.11.5)