FastQCFastQC Report
Wed 25 May 2016
SRR1294811_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294811_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132577
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48280.22639276330936703No Hit
GTACATGGGAAGCAGTGGTATCAAC41880.19638212359975749No Hit
CCCATGTACTCTGCGTTGATACCAC41000.19225566063968616No Hit
GGTATCAACGCAGAGTACTTTTTTT29490.13828340078693524No Hit
ATTCCATTCCATTCCATTCCATTCC23170.10864789407369581No Hit
TATCAACGCAGAGTACTTTTTTTTT22600.1059750714745587No Hit
GAGTACATGGGAAGCAGTGGTATCA21490.10077010114992331No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACG350.00219100716.2628659
AGGACCG655.2742005E-513.2010095
GCGGCGT752.1012734E-411.38427311
ACCGTCT752.1017311E-411.3840068
TTAGACA2350.011.3597324
GCGTCGC600.00593757211.06804214
ATCGTGC600.00593860811.0677828
TCGTGCA600.00593860811.0677829
ACCGTCA1053.5254088E-610.84216312
CCTAGAC2400.010.3285673
GTCTAGG2150.010.2025941
AGACCGT856.647711E-410.050136
CTAGACA2400.09.93131354
CTAGGGC1701.5706973E-89.5340613
TTTTTCG800.00454541679.49268119
CGTTCCG800.0045692219.48667110
ATTATAC2252.5465852E-119.3221933
GTCTTAT1951.5679689E-99.2926641
GTCTTAA3200.09.239171
TAGACAG4050.09.1809485
Produced by FastQC (version 0.11.5)