FastQCFastQC Report
Wed 25 May 2016
SRR1294810_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294810_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2483143
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC79750.32116555510496175No Hit
CCCATGTACTCTGCGTTGATACCAC77750.3131112465129878No Hit
GAGTACATGGGAAGCAGTGGTATCA41100.16551604156506491No Hit
CATGTACTCTGCGTTGATACCACTG37410.15065584221287295No Hit
GCGTTGATACCACTGCTTCCCATGT34420.1386146508678719No Hit
GTATCAACGCAGAGTACATGGGAAG32560.13112414387733612No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG551.9566723E-413.8216914
CGAATAG604.10082E-412.66400310
ACCGTCC802.875339E-511.8720248
ACCGGAA802.877715E-511.87106511
ACACCGT1052.7382157E-711.7565846
TAACCCG752.0702631E-411.4026665
CCGCGTG600.005888304711.0805559
GCACCGT1201.2843338E-711.078326
TAAGCCC1951.4551915E-1110.2333674
TACTCCG1356.425853E-79.8541565
TAGACTG3150.09.6530495
TCTATAC3000.09.501073
ACCGTCT900.0011165629.4976198
CACCGTC900.0011165629.4976197
GGAGCGT1201.710267E-59.4957036
GGAACGT1106.876064E-59.49531918
GGACAGT3450.09.3580846
TAGACAC2550.09.3159045
AGACCGT1151.1164852E-49.0828466
TGCACCG850.00742311738.9432675
Produced by FastQC (version 0.11.5)