FastQCFastQC Report
Wed 25 May 2016
SRR1294810_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294810_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2483143
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC83440.3360257544571537No Hit
CCCATGTACTCTGCGTTGATACCAC79020.31822573246889124No Hit
GTATCAACGCAGAGTACTTTTTTTT61030.24577722668408544No Hit
GAGTACATGGGAAGCAGTGGTATCA44460.17904727999958117No Hit
GGTATCAACGCAGAGTACTTTTTTT36300.1461857009443274No Hit
CATGTACTCTGCGTTGATACCACTG36050.14517891237033068No Hit
GTATCAACGCAGAGTACATGGGAAG34540.1390979093833903No Hit
GCGTTGATACCACTGCTTCCCATGT34330.13825220698123306No Hit
TATCAACGCAGAGTACTTTTTTTTT28800.11598204372442505No Hit
ACGCAGAGTACATGGGAAGCAGTGG25320.10196754677439035No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCTA655.5189175E-513.13753514
CGTGTAT907.5726857E-611.59624610
CAATGCG600.00593364211.0691439
ATAGACG1252.1462438E-710.6736633
GTACTAG1201.4568395E-610.32819751
CTGGTCG1201.5498663E-610.278499
CGGATAG750.00258001110.1653955
ACTGCGT1054.1651052E-59.9396398
CTCGTCT1502.697834E-79.4882213
TAGACGT1252.6543512E-59.1488544
GCACCGT1151.1197092E-49.0804416
TGCACCG1054.354154E-49.0762445
GTGCTAG2850.09.0319451
TACACCG950.00177255929.0284755
GTTCCGG850.00722428928.9729141
TAGGACA2451.6370905E-118.9465844
CGAGCAT850.0075159288.929728518
TTAGGCT2159.804353E-108.8651694
GTATAAC2053.8871804E-98.8361311
TTACACT3350.08.8188584
Produced by FastQC (version 0.11.5)