FastQCFastQC Report
Wed 25 May 2016
SRR1294809_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294809_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64198
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC39536.157512695099536No Hit
GTACATGGGAAGCAGTGGTATCAAC38145.94099504657466No Hit
CATGTACTCTGCGTTGATACCACTG19333.010997227327954No Hit
GAGTACATGGGAAGCAGTGGTATCA18662.9066326053771148No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA15472.409732390417147No Hit
GCGTTGATACCACTGCTTCCCATGT14412.244618212405371No Hit
ACGCAGAGTACATGGGAAGCAGTGG14082.193214741892271No Hit
ACTCTGCGTTGATACCACTGCTTCC13462.096638524564628No Hit
GGTATCAACGCAGAGTACATGGGAA12311.9175052182310977No Hit
GTATCAACGCAGAGTACATGGGAAG11971.864544066793358No Hit
TATCAACGCAGAGTACATGGGAAGC11831.8427365338484065No Hit
GTGGTATCAACGCAGAGTACATGGG9021.405028194024736No Hit
GCTTCCCATGTACTCTGCGTTGATA8231.2819714009782237No Hit
GATACCACTGCTTCCCATGTACTCT8001.2461447397115175No Hit
GTACTCTGCGTTGATACCACTGCTT7861.224337206766566No Hit
ATACCACTGCTTCCCATGTACTCTG7731.2040873547462538No Hit
CTGCTTCCCATGTACTCTGCGTTGA7111.107511137418611No Hit
CAGTGGTATCAACGCAGAGTACATG6461.0062618773170504No Hit
GGGAAGCAGTGGTATCAACGCAGAG6260.9751082588242624No Hit
CATGGGAAGCAGTGGTATCAACGCA6150.957973768653229No Hit
GCAGTGGTATCAACGCAGAGTACAT5540.8629552322502257No Hit
ACATGGGAAGCAGTGGTATCAACGC5500.8567245085516684No Hit
TACCACTGCTTCCCATGTACTCTGC5450.8489361039284713No Hit
CCATGTACTCTGCGTTGATACCACT5330.8302439328327984No Hit
GCAGAGTACATGGGAAGCAGTGGTA5330.8302439328327984No Hit
GTTGATACCACTGCTTCCCATGTAC5310.8271285709835198No Hit
CTTCCCATGTACTCTGCGTTGATAC5290.824013209134241No Hit
ATGGGAAGCAGTGGTATCAACGCAG4890.7617059721486651No Hit
CCACTGCTTCCCATGTACTCTGCGT4540.7071871397862862No Hit
AAGCAGTGGTATCAACGCAGAGTAC4270.6651297548210224No Hit
TTCCCATGTACTCTGCGTTGATACC3730.5810149848904951No Hit
TGATACCACTGCTTCCCATGTACTC3630.565438175644101No Hit
ATCAACGCAGAGTACATGGGAAGCA3450.5373999190005919No Hit
GGAAGCAGTGGTATCAACGCAGAGT2590.4034393594816038No Hit
GTATCAACGCAGAGTACTTTTTTTT2580.4018816785569644No Hit
GGTATCAACGCAGAGTACTTTTTTT2490.3878625502352098No Hit
GTACATGGTAAGCAGTGGTATCAAC2260.3520358889685037No Hit
CTGCGTTGATACCACTGCTTCCCAT2160.3364590797221097No Hit
TGGGAAGCAGTGGTATCAACGCAGA2050.31932458955107634No Hit
ACCATGTACTCTGCGTTGATACCAC2010.31309386585251875No Hit
CTCTGCGTTGATACCACTGCTTCCC1960.3053054612293218No Hit
TCAACGCAGAGTACATGGGAAGCAG1800.28038256643509146No Hit
ATGTACTCTGCGTTGATACCACTGC1720.2679211190379763No Hit
TATCAACGCAGAGTACTTTTTTTTT1700.2648057571886975No Hit
AAAAAGTACTCTGCGTTGATACCAC1690.2632480762640581No Hit
TCCCATGTACTCTGCGTTGATACCA1540.2398828623944671No Hit
TTGATACCACTGCTTCCCATGTACT1490.2320944577712701No Hit
TCCATGTACTCTGCGTTGATACCAC1380.21495996760023678No Hit
AAACAAAAAAAAAAAAAAAAAAAAA1380.21495996760023678No Hit
AAAGTACTCTGCGTTGATACCACTG1370.21340228667559735No Hit
GGTAAGCAGTGGTATCAACGCAGAG1350.21028692482631858No Hit
AGTGGTATCAACGCAGAGTACATGG1310.20405620112776102No Hit
AACGCAGAGTACATGGGAAGCAGTG1290.2009408392784822No Hit
GTACATGGGTAAGCAGTGGTATCAA1100.17134490171033365No Hit
CAACGCAGAGTACATGGGAAGCAGT1070.16667185893641548No Hit
CGCAGAGTACATGGGAAGCAGTGGT1030.16044113523785788No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1030.16044113523785788No Hit
CCCCATGTACTCTGCGTTGATACCA1020.1588834543132185No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA1000.1557680924639397No Hit
CTGCTTACCATGTACTCTGCGTTGA980.1526527306146609No Hit
GAGTACATGGTAAGCAGTGGTATCA980.1526527306146609No Hit
ACTCTGCGTTGATACCACTGCTTAC940.1464220069161033No Hit
ACTGCTTCCCATGTACTCTGCGTTG900.14019128321754573No Hit
TGTACTCTGCGTTGATACCACTGCT860.13396055951898814No Hit
ACGCAGAGTACATGGTAAGCAGTGG780.12149911212187296No Hit
GCTTACCATGTACTCTGCGTTGATA780.12149911212187296No Hit
GTACATGGAAAGCAGTGGTATCAAC770.11994143119723355No Hit
CACTGCTTCCCATGTACTCTGCGTT740.11526838842331538No Hit
CGTTGATACCACTGCTTCCCATGTA730.11371070749867597No Hit
CAGAGTACATGGGAAGCAGTGGTAT650.1012492601015608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA601.4207308E-615.8261821
ACATGGT1053.3178167E-610.8522413
TGGTAAG1053.3178167E-610.8522416
CATGGTA1053.3178167E-610.8522414
ATGGTAA1201.4478628E-610.2870195
TAAGCAG1304.189109E-58.765279
GTAAGCA1304.189109E-58.765278
GGTAAGC1304.189109E-58.765277
AACAAAA1701.3729943E-57.81999642
GAAAAAA1150.0099708547.43142561
AGTGGTA13000.06.50090914
CAGTGGT13200.06.402410513
GTGGTAT13450.06.283406315
TGGTATC13500.06.260134716
ACAAAAA2301.0611261E-46.19285443
GTATCAA13900.06.079986618
AGCAGTG13900.06.079986611
GCAGTGG14050.06.015075712
AAGCAGT13900.06.01167210
GAAGCAG11750.05.98027669
Produced by FastQC (version 0.11.5)