##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294809_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 64198 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.033801676064677 33.0 31.0 34.0 26.0 34.0 2 31.212452101311566 34.0 31.0 34.0 27.0 34.0 3 31.498177513318172 34.0 31.0 34.0 28.0 34.0 4 34.45573070812175 37.0 35.0 37.0 31.0 37.0 5 34.920013084519766 37.0 35.0 37.0 32.0 37.0 6 34.83787656936353 37.0 36.0 37.0 31.0 37.0 7 34.960357020467924 37.0 36.0 37.0 31.0 37.0 8 34.952334963706036 37.0 37.0 37.0 31.0 37.0 9 36.46964079877878 39.0 39.0 39.0 31.0 39.0 10 36.63511324340322 39.0 39.0 39.0 31.0 39.0 11 36.59302470481946 39.0 39.0 39.0 32.0 39.0 12 36.68368173463348 39.0 39.0 39.0 32.0 39.0 13 36.43350260132714 39.0 38.0 39.0 31.0 39.0 14 37.9438300258575 41.0 39.0 41.0 31.0 41.0 15 37.876880899716504 41.0 39.0 41.0 31.0 41.0 16 37.78884077385588 41.0 39.0 41.0 31.0 41.0 17 37.894545001401916 41.0 39.0 41.0 32.0 41.0 18 37.87585283030624 41.0 39.0 41.0 31.0 41.0 19 37.820601887909284 41.0 39.0 41.0 31.0 41.0 20 37.87339169444531 41.0 39.0 41.0 31.0 41.0 21 37.84063366460014 41.0 39.0 41.0 31.0 41.0 22 37.90414031589769 41.0 39.0 41.0 31.0 41.0 23 37.902286675597374 41.0 39.0 41.0 30.0 41.0 24 38.02205676189289 41.0 39.0 41.0 30.0 41.0 25 37.161827471260786 41.0 38.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1654384109573961 1101 2 0.3039524986291475 1101 3 -0.17045721140399195 1101 4 -0.2092217002215051 1101 5 -0.016203590443510052 1101 6 0.2060991360278095 1101 7 -0.18802034407228518 1101 8 -0.04329318660898451 1101 9 -0.07450582543734896 1101 10 0.05637485073218329 1101 11 -0.11192142571339048 1101 12 0.13788492899305993 1101 13 -0.14086039770855763 1101 14 0.4612509910715019 1101 15 0.1617347678765242 1101 16 0.10994462645805214 1101 17 0.20951689741178825 1101 18 0.1861571724166282 1101 19 -0.11763722431861368 1101 20 0.039687060561419685 1101 21 0.1832854514472615 1101 22 -0.15807868438639616 1101 23 -0.11877316795340676 1101 24 0.005511137699400592 1101 25 0.269832823342135 1102 1 0.17825012452408373 1102 2 0.25579418245435903 1102 3 0.456744801865284 1102 4 -0.012813555632170903 1102 5 0.23042189571525995 1102 6 0.28136795323211317 1102 7 0.17516885748342048 1102 8 0.17107423503365737 1102 9 0.04789179524441778 1102 10 0.11780244167247389 1102 11 -0.30043663962324985 1102 12 -0.7134076813935835 1102 13 -0.1274767326433519 1102 14 -0.14696223244714446 1102 15 0.4633819881922392 1102 16 0.6792651480778176 1102 17 -0.015031260904386556 1102 18 -0.017457561053973336 1102 19 -0.16385090539845493 1102 20 0.3719908811974264 1102 21 0.09726394607091748 1102 22 0.04370580428896176 1102 23 0.01929592378760958 1102 24 0.705465381579522 1102 25 -0.6719425141092472 1103 1 -1.0151813855273417 1103 2 -1.1412371405937947 1103 3 -1.2393748802291533 1103 4 -1.2469182774648004 1103 5 -0.9849824988616405 1103 6 -1.1852052117982765 1103 7 -0.9972247844053115 1103 8 -0.8490286167662475 1103 9 -1.2944410705807385 1103 10 -1.2542171936711455 1103 11 -0.6669630538355023 1103 12 -0.7788588267886283 1103 13 -1.0159760313810793 1103 14 -1.5836148738683704 1103 15 -1.0653697649634353 1103 16 -1.2607769276585046 1103 17 -1.9509826400535175 1103 18 -1.1820204409043669 1103 19 -2.3415040143278603 1103 20 -1.6906552151092598 1103 21 -1.6319319398570897 1103 22 -1.0762100442383868 1103 23 -0.8287639172596073 1103 24 -1.122842238803834 1103 25 -1.1124520980269708 1104 1 -0.24110471418559243 1104 2 -0.045281086362845 1104 3 -0.3389541228658999 1104 4 -0.18485656638485892 1104 5 -0.1996856311664601 1104 6 -0.5250836596711181 1104 7 -0.7603150134843233 1104 8 -0.7213988832459108 1104 9 -0.4252264843254778 1104 10 -0.7209072357468784 1104 11 -0.6660280374727137 1104 12 -0.46609585343659177 1104 13 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1.0961658890071106 1108 23 1.374134633465033 1108 24 1.4552210023028849 1108 25 1.6428179941996461 1109 1 0.003949049255268733 1109 2 0.6826759028844656 1109 3 -0.04024444544654315 1109 4 -0.10517914703002162 1109 5 0.25762619679053245 1109 6 0.33609913602781205 1109 7 0.5953839112468629 1109 8 0.500536600625054 1109 9 0.5025154511583949 1109 10 0.37360889328537894 1109 11 0.019993467903631768 1109 12 0.4580976949505029 1109 13 0.4544587512276195 1109 14 0.3050807783055447 1109 15 1.0185432785148194 1109 16 0.43313611581975664 1109 17 0.2524956208160418 1109 18 0.242327385182584 1109 19 0.29023511610692054 1109 20 0.23543174141248358 1109 21 0.2532854514472618 1109 22 0.0159638688050876 1109 23 0.3208013001316985 1109 24 0.009766456848339544 1109 25 0.11493920632085519 1110 1 1.2795046048108247 1110 2 1.301194421402986 1110 3 1.3608666656645703 1110 4 1.4414875196366452 1110 5 0.8346632338275697 1110 6 1.0209139508426262 1110 7 1.1742728001357463 1110 8 1.1594254895139429 1110 9 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0.6044582900850486 2215 21 1.1921350089693874 2215 22 1.1113620988935864 2215 23 0.9788543974768302 2215 24 0.9009168993262122 2215 25 0.7752931886217453 2216 1 0.6220971974034164 2216 2 0.5419351621437265 2216 3 0.6479037027016012 2216 4 0.6822282603773857 2216 5 0.7235521227164554 2216 6 0.7523954323241071 2216 7 0.48908761495056297 2216 8 0.3446106746991333 2216 9 0.7091821178250584 2216 10 0.8436088932853778 2216 11 0.5866601345702946 2216 12 0.11365325050606145 2216 13 0.5403846771535399 2216 14 -0.0026969994722350066 2216 15 0.7937284637000062 2216 16 0.9686916713753106 2216 17 1.3239771022975262 2216 18 0.594549607404808 2216 19 1.2080128938846997 2216 20 0.9224687784495202 2216 21 0.8777298958917044 2216 22 1.2426305354717542 2216 23 1.179690189020583 2216 24 0.7616183087001929 2216 25 0.8086429100245596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47.0 3 10.0 4 5.0 5 4.0 6 5.0 7 19.0 8 5.0 9 14.0 10 39.0 11 70.0 12 77.0 13 124.0 14 240.0 15 373.0 16 504.0 17 603.0 18 580.0 19 562.0 20 515.0 21 481.0 22 409.0 23 393.0 24 373.0 25 291.0 26 298.0 27 281.0 28 268.0 29 268.0 30 312.0 31 340.0 32 487.0 33 624.0 34 1047.0 35 1541.0 36 2946.0 37 7251.0 38 42792.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.19414541432332 22.83880415647541 10.738600071663368 24.228450357537895 2 11.316191843230255 28.776484538334323 30.66433675539052 29.2429868630449 3 23.18433575161869 33.7795459864264 25.348311100709886 17.68780716124503 4 15.109058570224981 26.443972914781416 24.50463381907823 33.94233469591537 5 10.932355465397519 41.36537411250683 33.31044706249512 14.391823359600531 6 30.920067390490452 31.522213902408584 17.243853737676275 20.313864969424685 7 18.347035240160366 38.41317879038423 21.7322122209569 21.50757374849851 8 26.990860600767334 34.92311051498799 20.87713278642503 17.208896097819647 9 25.7636743736154 21.849355674123995 19.013073730849637 33.373896221410966 10 18.48378943426842 34.80669017380722 27.896526976003994 18.812993415920367 11 23.85987896936802 30.529041112982718 16.09582631480442 29.515253602844844 12 24.25632146255479 36.53111204704634 24.566739981593276 14.64582650880559 13 29.84115095340636 23.340511188091003 20.946852666729082 25.87148519177355 14 21.193354652523205 33.35621246392637 22.591061539661492 22.85937134388893 15 35.94315487333083 21.898789467115936 20.198427555222764 21.959628104330463 16 16.167477848496194 30.36939972544615 32.71246724073381 20.75065518532385 17 26.16685386247348 34.093036315986524 23.69742917758642 16.042680643953574 18 25.6505054286784 25.315112941470115 28.377324347934607 20.657057281916885 19 22.86984325040942 35.67183966310536 28.0823520237074 13.37596506277782 20 24.278493650744124 27.148918910486415 25.025740226513992 23.546847212255468 21 18.147639699229355 32.79460859255561 27.905525568625006 21.152226139590027 22 22.90090624073872 31.169378109840746 18.482007767777763 27.447707881642774 23 16.472974026986975 30.4718820684814 27.136728804305438 25.918415100226188 24 22.189289112834032 38.83281593685163 20.537260346629644 18.440634603684693 25 22.727058860431924 26.21665281284798 19.157478709555996 31.898809617164098 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1613.0 1 1613.0 2 1150.5 3 688.0 4 688.0 5 688.0 6 635.5 7 583.0 8 583.0 9 583.0 10 574.0 11 565.0 12 565.0 13 565.0 14 535.0 15 505.0 16 505.0 17 505.0 18 561.5 19 618.0 20 618.0 21 618.0 22 783.0 23 948.0 24 948.0 25 948.0 26 1130.5 27 1313.0 28 1313.0 29 1313.0 30 1474.0 31 1635.0 32 1635.0 33 1635.0 34 1748.5 35 1862.0 36 1862.0 37 1862.0 38 1941.0 39 2020.0 40 2020.0 41 2020.0 42 2947.5 43 3875.0 44 3875.0 45 3875.0 46 13813.0 47 23751.0 48 23751.0 49 23751.0 50 19904.5 51 16058.0 52 16058.0 53 16058.0 54 10507.0 55 4956.0 56 4956.0 57 4956.0 58 3172.0 59 1388.0 60 1388.0 61 1388.0 62 1131.0 63 874.0 64 874.0 65 874.0 66 668.0 67 462.0 68 462.0 69 462.0 70 361.0 71 260.0 72 260.0 73 260.0 74 193.0 75 126.0 76 126.0 77 126.0 78 95.0 79 64.0 80 64.0 81 64.0 82 44.0 83 24.0 84 24.0 85 24.0 86 16.5 87 9.0 88 9.0 89 9.0 90 5.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014019128321754573 2 0.16199881616249726 3 0.16044113523785788 4 0.16199881616249726 5 0.17601794448425184 6 0.1464220069161033 7 0.14797968784074272 8 0.12461447397115176 9 0.1557680924639397 10 0.16199881616249726 11 0.12772983582043054 12 0.14174896414218513 13 0.17446026355961244 14 0.14486432599146393 15 0.1464220069161033 16 0.1464220069161033 17 0.1464220069161033 18 0.1464220069161033 19 0.12928751674506994 20 0.1495373687653821 21 0.1495373687653821 22 0.1355182404436275 23 0.14486432599146393 24 0.14797968784074272 25 0.6807065640674164 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 64198.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.678463503535934 #Duplication Level Percentage of deduplicated Percentage of total 1 88.84362609309652 25.478986884326616 2 6.311444245288143 3.6200504688619586 3 1.569713758079409 1.3505093616623571 4 0.8418880017380913 0.9657621732764261 5 0.4508174460920102 0.6464375837253498 6 0.2824398457443919 0.48599644848749185 7 0.2226929552984629 0.44705442537150686 8 0.13035685188202706 0.2990747375307642 9 0.09233610341643582 0.2383251814698277 >10 0.7984357177774157 4.483005701112184 >50 0.14121992287219595 2.8489984111654567 >100 0.16837760034761828 10.262001931524347 >500 0.08690456792135137 16.288669428954172 >1k 0.05974689044592906 32.58512726253154 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 3953 6.157512695099536 No Hit GTACATGGGAAGCAGTGGTATCAAC 3814 5.94099504657466 No Hit CATGTACTCTGCGTTGATACCACTG 1933 3.010997227327954 No Hit GAGTACATGGGAAGCAGTGGTATCA 1866 2.9066326053771148 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1547 2.409732390417147 No Hit GCGTTGATACCACTGCTTCCCATGT 1441 2.244618212405371 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1408 2.193214741892271 No Hit ACTCTGCGTTGATACCACTGCTTCC 1346 2.096638524564628 No Hit GGTATCAACGCAGAGTACATGGGAA 1231 1.9175052182310977 No Hit GTATCAACGCAGAGTACATGGGAAG 1197 1.864544066793358 No Hit TATCAACGCAGAGTACATGGGAAGC 1183 1.8427365338484065 No Hit GTGGTATCAACGCAGAGTACATGGG 902 1.405028194024736 No Hit GCTTCCCATGTACTCTGCGTTGATA 823 1.2819714009782237 No Hit GATACCACTGCTTCCCATGTACTCT 800 1.2461447397115175 No Hit GTACTCTGCGTTGATACCACTGCTT 786 1.224337206766566 No Hit ATACCACTGCTTCCCATGTACTCTG 773 1.2040873547462538 No Hit CTGCTTCCCATGTACTCTGCGTTGA 711 1.107511137418611 No Hit CAGTGGTATCAACGCAGAGTACATG 646 1.0062618773170504 No Hit GGGAAGCAGTGGTATCAACGCAGAG 626 0.9751082588242624 No Hit CATGGGAAGCAGTGGTATCAACGCA 615 0.957973768653229 No Hit GCAGTGGTATCAACGCAGAGTACAT 554 0.8629552322502257 No Hit ACATGGGAAGCAGTGGTATCAACGC 550 0.8567245085516684 No Hit TACCACTGCTTCCCATGTACTCTGC 545 0.8489361039284713 No Hit CCATGTACTCTGCGTTGATACCACT 533 0.8302439328327984 No Hit GCAGAGTACATGGGAAGCAGTGGTA 533 0.8302439328327984 No Hit GTTGATACCACTGCTTCCCATGTAC 531 0.8271285709835198 No Hit CTTCCCATGTACTCTGCGTTGATAC 529 0.824013209134241 No Hit ATGGGAAGCAGTGGTATCAACGCAG 489 0.7617059721486651 No Hit CCACTGCTTCCCATGTACTCTGCGT 454 0.7071871397862862 No Hit AAGCAGTGGTATCAACGCAGAGTAC 427 0.6651297548210224 No Hit TTCCCATGTACTCTGCGTTGATACC 373 0.5810149848904951 No Hit TGATACCACTGCTTCCCATGTACTC 363 0.565438175644101 No Hit ATCAACGCAGAGTACATGGGAAGCA 345 0.5373999190005919 No Hit GGAAGCAGTGGTATCAACGCAGAGT 259 0.4034393594816038 No Hit GTATCAACGCAGAGTACTTTTTTTT 258 0.4018816785569644 No Hit GGTATCAACGCAGAGTACTTTTTTT 249 0.3878625502352098 No Hit GTACATGGTAAGCAGTGGTATCAAC 226 0.3520358889685037 No Hit CTGCGTTGATACCACTGCTTCCCAT 216 0.3364590797221097 No Hit TGGGAAGCAGTGGTATCAACGCAGA 205 0.31932458955107634 No Hit ACCATGTACTCTGCGTTGATACCAC 201 0.31309386585251875 No Hit CTCTGCGTTGATACCACTGCTTCCC 196 0.3053054612293218 No Hit TCAACGCAGAGTACATGGGAAGCAG 180 0.28038256643509146 No Hit ATGTACTCTGCGTTGATACCACTGC 172 0.2679211190379763 No Hit TATCAACGCAGAGTACTTTTTTTTT 170 0.2648057571886975 No Hit AAAAAGTACTCTGCGTTGATACCAC 169 0.2632480762640581 No Hit TCCCATGTACTCTGCGTTGATACCA 154 0.2398828623944671 No Hit TTGATACCACTGCTTCCCATGTACT 149 0.2320944577712701 No Hit TCCATGTACTCTGCGTTGATACCAC 138 0.21495996760023678 No Hit AAACAAAAAAAAAAAAAAAAAAAAA 138 0.21495996760023678 No Hit AAAGTACTCTGCGTTGATACCACTG 137 0.21340228667559735 No Hit GGTAAGCAGTGGTATCAACGCAGAG 135 0.21028692482631858 No Hit AGTGGTATCAACGCAGAGTACATGG 131 0.20405620112776102 No Hit AACGCAGAGTACATGGGAAGCAGTG 129 0.2009408392784822 No Hit GTACATGGGTAAGCAGTGGTATCAA 110 0.17134490171033365 No Hit CAACGCAGAGTACATGGGAAGCAGT 107 0.16667185893641548 No Hit CGCAGAGTACATGGGAAGCAGTGGT 103 0.16044113523785788 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 103 0.16044113523785788 No Hit CCCCATGTACTCTGCGTTGATACCA 102 0.1588834543132185 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 100 0.1557680924639397 No Hit CTGCTTACCATGTACTCTGCGTTGA 98 0.1526527306146609 No Hit GAGTACATGGTAAGCAGTGGTATCA 98 0.1526527306146609 No Hit ACTCTGCGTTGATACCACTGCTTAC 94 0.1464220069161033 No Hit ACTGCTTCCCATGTACTCTGCGTTG 90 0.14019128321754573 No Hit TGTACTCTGCGTTGATACCACTGCT 86 0.13396055951898814 No Hit ACGCAGAGTACATGGTAAGCAGTGG 78 0.12149911212187296 No Hit GCTTACCATGTACTCTGCGTTGATA 78 0.12149911212187296 No Hit GTACATGGAAAGCAGTGGTATCAAC 77 0.11994143119723355 No Hit CACTGCTTCCCATGTACTCTGCGTT 74 0.11526838842331538 No Hit CGTTGATACCACTGCTTCCCATGTA 73 0.11371070749867597 No Hit CAGAGTACATGGGAAGCAGTGGTAT 65 0.1012492601015608 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0015576809246393968 0.0 11 0.0 0.0 0.0 0.0015576809246393968 0.0 12 0.0 0.0 0.0 0.0031153618492787936 0.0 13 0.0 0.0 0.0 0.0031153618492787936 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACAAA 60 1.4207308E-6 15.826182 1 ACATGGT 105 3.3178167E-6 10.852241 3 TGGTAAG 105 3.3178167E-6 10.852241 6 CATGGTA 105 3.3178167E-6 10.852241 4 ATGGTAA 120 1.4478628E-6 10.287019 5 TAAGCAG 130 4.189109E-5 8.76527 9 GTAAGCA 130 4.189109E-5 8.76527 8 GGTAAGC 130 4.189109E-5 8.76527 7 AACAAAA 170 1.3729943E-5 7.8199964 2 GAAAAAA 115 0.009970854 7.4314256 1 AGTGGTA 1300 0.0 6.500909 14 CAGTGGT 1320 0.0 6.4024105 13 GTGGTAT 1345 0.0 6.2834063 15 TGGTATC 1350 0.0 6.2601347 16 ACAAAAA 230 1.0611261E-4 6.1928544 3 GTATCAA 1390 0.0 6.0799866 18 AGCAGTG 1390 0.0 6.0799866 11 GCAGTGG 1405 0.0 6.0150757 12 AAGCAGT 1390 0.0 6.011672 10 GAAGCAG 1175 0.0 5.9802766 9 >>END_MODULE