FastQCFastQC Report
Wed 25 May 2016
SRR1294809_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294809_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64198
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC39686.180877908969127No Hit
GTACATGGGAAGCAGTGGTATCAAC38906.0593787968472546No Hit
GAGTACATGGGAAGCAGTGGTATCA21413.334994859652949No Hit
CATGTACTCTGCGTTGATACCACTG19242.9969780990061996No Hit
ACGCAGAGTACATGGGAAGCAGTGG15352.391040219321474No Hit
GCGTTGATACCACTGCTTCCCATGT13872.1605034424748433No Hit
TATCAACGCAGAGTACATGGGAAGC13022.028100563880495No Hit
ACTCTGCGTTGATACCACTGCTTCC13002.0249852020312162No Hit
GGTATCAACGCAGAGTACATGGGAA12661.9720240505934763No Hit
GTATCAACGCAGAGTACATGGGAAG12291.9143898563818187No Hit
GTGGTATCAACGCAGAGTACATGGG9701.510950496900215No Hit
GTATCAACGCAGAGTACTTTTTTTT8511.3255864668681268No Hit
GCTTCCCATGTACTCTGCGTTGATA8171.2726253154303873No Hit
GATACCACTGCTTCCCATGTACTCT7911.2321256113897627No Hit
GGGAAGCAGTGGTATCAACGCAGAG7611.1853951836505812No Hit
GTACTCTGCGTTGATACCACTGCTT7461.1620299697809902No Hit
CAGTGGTATCAACGCAGAGTACATG7371.1480108414592354No Hit
ATACCACTGCTTCCCATGTACTCTG7121.1090688183432504No Hit
GGTATCAACGCAGAGTACTTTTTTT7101.1059534564939717No Hit
CATGGGAAGCAGTGGTATCAACGCA6931.079472880775102No Hit
ACATGGGAAGCAGTGGTATCAACGC6320.9844543443720988No Hit
CTGCTTCCCATGTACTCTGCGTTGA6020.937723916632917No Hit
TACCACTGCTTCCCATGTACTCTGC5810.9050126172154896No Hit
TATCAACGCAGAGTACTTTTTTTTT5610.8738589987227017No Hit
GCAGTGGTATCAACGCAGAGTACAT5500.8567245085516684No Hit
GTTGATACCACTGCTTCCCATGTAC5460.8504937848531107No Hit
CCATGTACTCTGCGTTGATACCACT5400.8411476993052743No Hit
GCAGAGTACATGGGAAGCAGTGGTA5250.8177824854356833No Hit
ATGGGAAGCAGTGGTATCAACGCAG4790.746129162902271No Hit
AAGCAGTGGTATCAACGCAGAGTAC4650.7243216299573195No Hit
CCACTGCTTCCCATGTACTCTGCGT4520.7040717779370074No Hit
CTTCCCATGTACTCTGCGTTGATAC4420.6884949686906134No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4040.6293030935543164No Hit
TGATACCACTGCTTCCCATGTACTC3810.5934764322876102No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3810.5934764322876102No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3520.5483036854730677No Hit
ATCAACGCAGAGTACATGGGAAGCA3310.5155923860556404No Hit
TTCCCATGTACTCTGCGTTGATACC3240.5046886195831646No Hit
GGAAGCAGTGGTATCAACGCAGAGT2570.400323997632325No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2540.39565095485840684No Hit
GTACATGGTAAGCAGTGGTATCAAC2540.39565095485840684No Hit
TGGGAAGCAGTGGTATCAACGCAGA2060.32088227047571577No Hit
GTGGTATCAACGCAGAGTACTTTTT1900.29595937568148545No Hit
TCAACGCAGAGTACATGGGAAGCAG1860.28972865198292785No Hit
CTGCGTTGATACCACTGCTTCCCAT1830.28505560920900963No Hit
ACCATGTACTCTGCGTTGATACCAC1830.28505560920900963No Hit
CTCTGCGTTGATACCACTGCTTCCC1760.27415184273653387No Hit
AAAAAGTACTCTGCGTTGATACCAC1560.24299822424374592No Hit
ATGTACTCTGCGTTGATACCACTGC1450.22586373407271254No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1420.22119069129879435No Hit
TCCCATGTACTCTGCGTTGATACCA1420.22119069129879435No Hit
AGTGGTATCAACGCAGAGTACATGG1410.21963301037415495No Hit
CCCCATGTACTCTGCGTTGATACCA1380.21495996760023678No Hit
AACGCAGAGTACATGGGAAGCAGTG1350.21028692482631858No Hit
GGTAAGCAGTGGTATCAACGCAGAG1280.1993831583538428No Hit
CAACGCAGAGTACATGGGAAGCAGT1270.19782547742920342No Hit
AAAGTACTCTGCGTTGATACCACTG1240.19315243465528523No Hit
TTGATACCACTGCTTCCCATGTACT1230.1915947537306458No Hit
TCCATGTACTCTGCGTTGATACCAC1200.18692171095672763No Hit
ATCAACGCAGAGTACTTTTTTTTTT1110.17290258263497305No Hit
ACTGCTTCCCATGTACTCTGCGTTG1090.16978722078569425No Hit
GAGTACATGGTAAGCAGTGGTATCA1070.16667185893641548No Hit
GCTTACCATGTACTCTGCGTTGATA1010.1573257733885791No Hit
GTACATGGGTAAGCAGTGGTATCAA970.1510950496900215No Hit
GTACATGGAAAGCAGTGGTATCAAC930.14486432599146393No Hit
GAGTACATGGAAAGCAGTGGTATCA860.13396055951898814No Hit
CGTTGATACCACTGCTTCCCATGTA840.13084519766970934No Hit
ACTCTGCGTTGATACCACTGCTTAC800.12461447397115176No Hit
TGTACTCTGCGTTGATACCACTGCT780.12149911212187296No Hit
GCGTTGATACCACTGCTTACCATGT770.11994143119723355No Hit
CGCAGAGTACATGGGAAGCAGTGGT770.11994143119723355No Hit
ACGCAGAGTACATGGTAAGCAGTGG730.11371070749867597No Hit
CTGCTTACCATGTACTCTGCGTTGA680.10592230287547898No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT680.10592230287547898No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT650.1012492601015608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTAAG1000.00285886658.5394776
ATGGTAA1150.0096282747.4605225
CATGGTA1150.0096282747.4605224
CCATGTA12800.07.2986192
TACTCTG13850.07.05628737
TGCGTTG14000.06.912910512
TGTACTC13700.06.88873055
CTGCGTT14100.06.86388311
TGATACC14350.06.81042417
CTCTGCG14250.06.7916319
TCTGCGT14250.06.79163110
CCCATGT12650.06.7822931
ATGTACT13900.06.72102984
TTGATAC14650.06.67096116
CGTTGAT14550.06.65159814
GTACTCT14750.06.6257346
GATACCA14800.06.603349718
ACTCTGC14850.06.5172228
ATACCAC15100.06.472157519
GCGTTGA15250.06.346278713
Produced by FastQC (version 0.11.5)