FastQCFastQC Report
Wed 25 May 2016
SRR1294808_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294808_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136524
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC84426.183528170871055No Hit
GTACATGGGAAGCAGTGGTATCAAC83436.111013448184935No Hit
GAGTACATGGGAAGCAGTGGTATCA42143.0866367818112566No Hit
CATGTACTCTGCGTTGATACCACTG41723.0558729600656296No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA34042.4933345052884475No Hit
GCGTTGATACCACTGCTTCCCATGT30732.2508862910550524No Hit
ACGCAGAGTACATGGGAAGCAGTGG30032.1996132548123404No Hit
ACTCTGCGTTGATACCACTGCTTCC27121.986463918431924No Hit
GTATCAACGCAGAGTACATGGGAAG25111.8392370572207086No Hit
TATCAACGCAGAGTACATGGGAAGC25031.8333772816501128No Hit
GGTATCAACGCAGAGTACATGGGAA24691.8084732354750814No Hit
GTGGTATCAACGCAGAGTACATGGG18701.3697225396267323No Hit
GCTTCCCATGTACTCTGCGTTGATA17681.2950104011016377No Hit
GTACTCTGCGTTGATACCACTGCTT16731.2254255662008144No Hit
ATACCACTGCTTCCCATGTACTCTG15411.1287392692859863No Hit
GATACCACTGCTTCCCATGTACTCT14781.0825935366675457No Hit
GGGAAGCAGTGGTATCAACGCAGAG14101.0327854443174826No Hit
CTGCTTCCCATGTACTCTGCGTTGA13650.9998242067328821No Hit
CATGGGAAGCAGTGGTATCAACGCA12150.8899534147842139No Hit
CAGTGGTATCAACGCAGAGTACATG11850.8679792563944801No Hit
GCAGTGGTATCAACGCAGAGTACAT11800.8643168966628579No Hit
ACATGGGAAGCAGTGGTATCAACGC11490.8416102663267996No Hit
GTTGATACCACTGCTTCCCATGTAC11350.8313556590782574No Hit
CCATGTACTCTGCGTTGATACCACT11160.8174386920980926No Hit
TACCACTGCTTCCCATGTACTCTGC10950.802056781225279No Hit
GCAGAGTACATGGGAAGCAGTGGTA10570.7742228472649497No Hit
CCACTGCTTCCCATGTACTCTGCGT10210.7478538571972693No Hit
CTTCCCATGTACTCTGCGTTGATAC9410.6892561014913129No Hit
ATGGGAAGCAGTGGTATCAACGCAG9380.6870586856523395No Hit
AAGCAGTGGTATCAACGCAGAGTAC8650.6335882335706543No Hit
GTATCAACGCAGAGTACTTTTTTTT8130.5954996923617826No Hit
TTCCCATGTACTCTGCGTTGATACC7950.5823151973279423No Hit
TGATACCACTGCTTCCCATGTACTC7670.5618059828308576No Hit
ATCAACGCAGAGTACATGGGAAGCA6530.47830418094986965No Hit
GGTATCAACGCAGAGTACTTTTTTT6400.4687820456476517No Hit
GTACATGGTAAGCAGTGGTATCAAC5280.38674518765931265No Hit
GGAAGCAGTGGTATCAACGCAGAGT4940.36184114148428115No Hit
ACCATGTACTCTGCGTTGATACCAC4790.3508540622894143No Hit
TATCAACGCAGAGTACTTTTTTTTT4550.3332747355776274No Hit
TCAACGCAGAGTACATGGGAAGCAG4010.29372125047610675No Hit
TGGGAAGCAGTGGTATCAACGCAGA3930.2878614749055111No Hit
CTCTGCGTTGATACCACTGCTTCCC3770.27614192376431984No Hit
CTGCGTTGATACCACTGCTTCCCAT3750.2746769798716709No Hit
AAAAAGTACTCTGCGTTGATACCAC3630.26588731651577746No Hit
AAACAAAAAAAAAAAAAAAAAAAAA3300.24171574228707043No Hit
TCCCATGTACTCTGCGTTGATACCA3090.22633383141425684No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3070.22486888752160794No Hit
ATGTACTCTGCGTTGATACCACTGC3060.22413641557528347No Hit
TCCATGTACTCTGCGTTGATACCAC2950.21607922416571448No Hit
TTGATACCACTGCTTCCCATGTACT2930.21461428027306556No Hit
AAAGTACTCTGCGTTGATACCACTG2910.21314933638041664No Hit
GGTAAGCAGTGGTATCAACGCAGAG2660.19483753772230522No Hit
CCCCATGTACTCTGCGTTGATACCA2590.18971023409803403No Hit
GAGTACATGGTAAGCAGTGGTATCA2520.18458293047376287No Hit
AGTGGTATCAACGCAGAGTACATGG2450.17945562684949168No Hit
AACGCAGAGTACATGGGAAGCAGTG2400.17579326711786938No Hit
ACTGCTTCCCATGTACTCTGCGTTG2220.16260877208402919No Hit
GTACATGGAAAGCAGTGGTATCAAC2100.15381910872813573No Hit
CAACGCAGAGTACATGGGAAGCAGT2000.14649438926489117No Hit
CGCAGAGTACATGGGAAGCAGTGGT1990.1457619173185667No Hit
GTACATGGGTAAGCAGTGGTATCAA1990.1457619173185667No Hit
GCTTACCATGTACTCTGCGTTGATA1990.1457619173185667No Hit
TGTACTCTGCGTTGATACCACTGCT1960.14356450147959332No Hit
ACTCTGCGTTGATACCACTGCTTAC1960.14356450147959332No Hit
CGTTGATACCACTGCTTCCCATGTA1780.13038000644575312No Hit
CTGCTTACCATGTACTCTGCGTTGA1700.12452023087515748No Hit
GCGTTGATACCACTGCTTACCATGT1620.11866045530456183No Hit
ACGCAGAGTACATGGTAAGCAGTGG1530.11206820778764173No Hit
AAAAACAAAAAAAAAAAAAAAAAAA1480.10840584805601944No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA1440.10547596027072163No Hit
GTACTTTTTTTTTTTTTTTTTTTTT1420.10401101637807272No Hit
GAGTACATGGAAAGCAGTGGTATCA1370.10034865664645044No Hit
GTGGTATCAACGCAGAGTACTTTTT1370.10034865664645044No Hit
GCTTTCCATGTACTCTGCGTTGATA1370.10034865664645044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA855.4569682E-1116.7611451
AAAAAGT750.002625795310.1311811
AAAAGTA850.00737536058.9392782
GAAAAAA2355.820766E-108.4875581
CATGGGT1050.00446701148.1411284
AAGTACT1100.0067846217.77107672
CATGGTA1502.2707593E-47.59838534
CCATGTA27050.07.02253772
ACATGGT1709.527974E-46.7044583
GAAGCAG24800.06.5873119
AGTGGTA27100.06.518910414
CAGTGGT27150.06.506904613
GGAAGCA25300.06.45712668
CCCATGT27500.06.42409041
TGTACTC29750.06.38519865
ATGTACT29850.06.3638074
TACTCTG31100.06.35234837
TGATACC31300.06.31175817
TGCGTTG31600.06.281893712
CTGCGTT31650.06.271969311
Produced by FastQC (version 0.11.5)