##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294808_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	136524
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.90358471770531	33.0	31.0	34.0	26.0	34.0
2	31.05012305528698	33.0	31.0	34.0	26.0	34.0
3	31.319672731534382	34.0	31.0	34.0	27.0	34.0
4	34.28130585098591	37.0	35.0	37.0	30.0	37.0
5	34.82594269139492	37.0	35.0	37.0	31.0	37.0
6	34.72523512349477	37.0	36.0	37.0	31.0	37.0
7	34.93437783832879	37.0	36.0	37.0	31.0	37.0
8	34.8446793237819	37.0	37.0	37.0	31.0	37.0
9	36.36739327883742	39.0	39.0	39.0	30.0	39.0
10	36.55466438135419	39.0	38.0	39.0	31.0	39.0
11	36.533847528639654	39.0	39.0	39.0	31.0	39.0
12	36.63287773578272	39.0	39.0	39.0	31.0	39.0
13	36.328931176935924	39.0	38.0	39.0	31.0	39.0
14	37.844459582198	41.0	39.0	41.0	31.0	41.0
15	37.76097242975594	41.0	39.0	41.0	31.0	41.0
16	37.723301397556476	41.0	38.0	41.0	31.0	41.0
17	37.81480911781079	41.0	39.0	41.0	31.0	41.0
18	37.804122352113914	41.0	39.0	41.0	31.0	41.0
19	37.74534880314084	41.0	39.0	41.0	31.0	41.0
20	37.82166505522839	41.0	39.0	41.0	31.0	41.0
21	37.76754270311447	41.0	39.0	41.0	31.0	41.0
22	37.860017286337936	41.0	39.0	41.0	30.0	41.0
23	37.871128885763675	41.0	39.0	41.0	30.0	41.0
24	37.9955758694442	41.0	39.0	41.0	30.0	41.0
25	37.180495737013274	41.0	38.0	41.0	28.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.908752083523968
1101	2	1.0135141297471755
1101	3	1.0515371227506876
1101	4	0.9512125550307573
1101	5	0.6827716414618195
1101	6	0.9710084370652368
1101	7	0.9228270812941162
1101	8	0.5418734561402374
1101	9	1.1677649356153168
1101	10	0.7319691956194561
1101	11	1.1963344340852373
1101	12	0.9600333286617371
1101	13	1.3689294018503517
1101	14	1.2441952803402359
1101	15	1.0936885440160893
1101	16	0.7711120334550827
1101	17	1.0171132022647313
1101	18	0.8633856965193729
1101	19	0.44714735297753805
1101	20	0.9862086168793027
1101	21	0.6142427417000818
1101	22	1.0058179226373483
1101	23	1.1454325896169806
1101	24	1.1399204895732993
1101	25	1.029004541636887
1102	1	0.08607542163442972
1102	2	-0.0018921099176907319
1102	3	0.20402857903996363
1102	4	0.16497068533612946
1102	5	0.17814150145520813
1102	6	0.19184957916643697
1102	7	0.07286566579416842
1102	8	0.6104987455239836
1102	9	0.41947367776062094
1102	10	0.3831101944067967
1102	11	0.2645738784684326
1102	12	0.46369885616697104
1102	13	0.47018615413786335
1102	14	0.5535602897107523
1102	15	0.43488576593207995
1102	16	0.13245587647129042
1102	17	0.1944365403751931
1102	18	0.38066604047262587
1102	19	0.2567604055627015
1102	20	0.10039117668527808
1102	21	0.2637301190564898
1102	22	0.461555989002683
1102	23	0.4822201543838176
1102	24	0.2654579165383524
1102	25	0.5421783923699977
1103	1	0.468940955978173
1103	2	0.26482258349617993
1103	3	0.015782201826805675
1103	4	0.14452237412280766
1103	5	0.40725679335623255
1103	6	0.5895941692304447
1103	7	0.46191002588393815
1103	8	0.030381818866366928
1103	9	0.32543361237035384
1103	10	0.5408780589713729
1103	11	0.5533716396831423
1103	12	0.4389558454440774
1103	13	0.6186165107364587
1103	14	0.5118878505985123
1103	15	0.9911740372462603
1103	16	0.6719255503512045
1103	17	0.5238261388900582
1103	18	0.5673053688370402
1103	19	0.3623823057594251
1103	20	0.37012601362523867
1103	21	0.6641346520425557
1103	22	0.5930292094731655
1103	23	0.6427587928255392
1103	24	0.5311197158789085
1103	25	0.6877825596139076
1104	1	-0.4898103086554464
1104	2	-0.4244099645598993
1104	3	0.294464092273401
1104	4	-0.03015604761444024
1104	5	-0.20135716819890348
1104	6	-0.31533428863919255
1104	7	-0.566764638084841
1104	8	-0.46864983310646124
1104	9	-0.01357491487346607
1104	10	-0.13939365659446423
1104	11	-0.39665003975030544
1104	12	-0.3336124447712763
1104	13	-0.2901275274193438
1104	14	-0.07918022282250092
1104	15	-0.022585176512954774
1104	16	0.1352005900968365
1104	17	-0.2841518926173876
1104	18	-0.5966200539119129
1104	19	-0.2044915199379318
1104	20	-0.2549932232587082
1104	21	-0.2452742220722044
1104	22	-0.4729629859307991
1104	23	-0.5140268698424819
1104	24	-0.41433482957563683
1104	25	-0.7678902254706443
1105	1	-0.448557115163446
1105	2	-0.4040522934559583
1105	3	-0.39662102269230814
1105	4	-0.3171266002566355
1105	5	-0.07953385234574029
1105	6	-0.1025334280771375
1105	7	0.049639826080820626
1105	8	0.18085937759678217
1105	9	-0.3312029907104943
1105	10	-0.17577929596517805
1105	11	-0.1517125649620823
1105	12	-0.04126337928787649
1105	13	-0.024052497165207853
1105	14	-0.08297207437554732
1105	15	-0.44256635171800696
1105	16	0.25430775696519703
1105	17	-0.07153928000477805
1105	18	0.02145174923452231
1105	19	0.0793057101279615
1105	20	-0.32873263431154953
1105	21	-0.1981315570190887
1105	22	-0.021598188297339505
1105	23	-0.053572385507401066
1105	24	-0.0043845808194191704
1105	25	-0.162484820065238
1106	1	-0.4446531898141153
1106	2	-0.23475523806306597
1106	3	-0.5972208941484247
1106	4	-0.374757108004971
1106	5	-0.16915750442029776
1106	6	-0.4270115161058925
1106	7	0.08560830902494843
1106	8	-0.3833530530728382
1106	9	-0.3306316845618156
1106	10	-0.24212222248092274
1106	11	-0.34474262994815774
1106	12	0.15750355160788132
1106	13	-0.0019857971723453716
1106	14	-0.46024837231170324
1106	15	-0.4968642402657011
1106	16	-0.1929773486074211
1106	17	-0.508540874901108
1106	18	-0.468157620186318
1106	19	-0.5056165684313996
1106	20	-0.14127280427513256
1106	21	-0.2801377937778824
1106	22	-0.395994151067228
1106	23	-0.20384970703373995
1106	24	-0.30141616928855797
1106	25	-0.06679104016092907
1107	1	-0.404356010135821
1107	2	0.07410966059289237
1107	3	-0.2723429157396353
1107	4	-0.15752261015688163
1107	5	-0.28338894872315024
1107	6	-0.13668428184848125
1107	7	-0.08668858212939057
1107	8	0.05010610876506405
1107	9	0.301812834685137
1107	10	-0.011375185862426918
1107	11	-0.19776371624086408
1107	12	-0.23666826441000666
1107	13	0.1397515979371704
1107	14	-0.010295257455126716
1107	15	-0.05770673022747275
1107	16	0.2235069869459423
1107	17	0.2869196814692643
1107	18	-0.1314020721110154
1107	19	-0.02842198306436927
1107	20	0.002700651520598285
1107	21	0.06334998001933201
1107	22	-0.2700293990776146
1107	23	-0.07064781239306939
1107	24	-0.220364980329407
1107	25	-0.24288683011548784
1108	1	0.2801297671669829
1108	2	0.3989237764209008
1108	3	0.5748114079148365
1108	4	0.5534076065374478
1108	5	0.321695296030029
1108	6	0.5036213185517084
1108	7	0.3858096556659234
1108	8	0.566375237212732
1108	9	0.29910699780844396
1108	10	0.43886242754397387
1108	11	0.502775177321972
1108	12	0.053106400771099516
1108	13	0.10277940666406238
1108	14	0.47980228910118683
1108	15	-0.07999009274139723
1108	16	0.21943686256840067
1108	17	0.021975048803668074
1108	18	0.13990923555855517
1108	19	0.5289804135340077
1108	20	0.46062375118179943
1108	21	0.266355960146214
1108	22	0.1973072843394732
1108	23	0.05040996518258822
1108	24	0.20738462743793917
1108	25	0.12258305323793195
1109	1	0.8310030280457426
1109	2	1.004814587094561
1109	3	0.99471668131104
1109	4	1.1480707773413599
1109	5	0.7694687979541612
1109	6	0.7066914754426463
1109	7	0.4040360691644622
1109	8	0.9151086917735611
1109	9	0.749142943171492
1109	10	0.8725538048857118
1109	11	0.5378536227907418
1109	12	0.5370367090554495
1109	13	0.9858512551015011
1109	14	1.3352173523227364
1109	15	0.7124994408146534
1109	16	1.0526292336937004
1109	17	0.9393641467865024
1109	18	0.9173228609655837
1109	19	1.1159681921105715
1109	20	0.8758984160441443
1109	21	0.8452031712089294
1109	22	0.8608586862086298
1109	23	0.8136568886824023
1109	24	0.4108988152841775
1109	25	0.7814404135796238
1110	1	0.22106578482934225
1110	2	0.33284294051909313
1110	3	0.19181361570988287
1110	4	0.2757063365088115
1110	5	0.50356514541906
1110	6	0.39692579753393886
1110	7	0.2157657183585613
1110	8	0.24878064084575158
1110	9	0.2392779379793879
1110	10	0.11818566185441881
1110	11	0.24680210804967118
1110	12	-0.13470919369410694
1110	13	-0.1583087521223021
1110	14	0.3595976855206189
1110	15	0.5344327109397184
1110	16	0.13540949465356533
1110	17	-0.1923122268646793
1110	18	0.28837798760786626
1110	19	0.03734161754738352
1110	20	4.187104140953579E-4
1110	21	0.19266806804400005
1110	22	-0.1360260489938625
1110	23	-0.2941809369530688
1110	24	0.31416758588329685
1110	25	-0.14799206644205043
1111	1	-0.3377951355876405
1111	2	0.05392099798159222
1111	3	0.07542663376225889
1111	4	0.15315357780045247
1111	5	0.26106578106615075
1111	6	-0.09926031993364148
1111	7	-0.3242800482318273
1111	8	-0.6732254613663002
1111	9	-0.14961095090950494
1111	10	-0.06907597048730452
1111	11	-0.24067564430222177
1111	12	-0.15549485349578873
1111	13	-0.33133188967633487
1111	14	-0.2134381649751802
1111	15	-0.025572374236993767
1111	16	-0.0011910647158757115
1111	17	0.01618001824083848
1111	18	0.09005609592213659
1111	19	0.004351533641965943
1111	20	6.7296924959237E-4
1111	21	-0.19800063269335055
1111	22	-0.23251727706403358
1111	23	-0.29316390161108785
1111	24	-0.18538746115458338
1111	25	-0.03967780252137487
1112	1	-0.20791160322732694
1112	2	-0.07360369610657003
1112	3	-0.0059901434749498605
1112	4	0.07118394784320259
1112	5	-0.14048957791955274
1112	6	0.03497232049094379
1112	7	-0.10417095196174131
1112	8	-0.04448449129405674
1112	9	-0.1187986259795295
1112	10	-0.30289581421426703
1112	11	-0.4603793152442819
1112	12	-0.014443696190760136
1112	13	-0.4149969346416924
1112	14	0.07223926389109891
1112	15	-0.059628101791169286
1112	16	-0.10958705469081309
1112	17	-0.45669334162942476
1112	18	-0.5342989092378261
1112	19	-0.2774190693360694
1112	20	-0.16348743175291247
1112	21	-0.3710066772164211
1112	22	-0.4413481778453985
1112	23	-0.4932228725678911
1112	24	-0.4345028968025275
1112	25	-0.5406360805694419
1113	1	0.549177615438861
1113	2	0.7975566829386658
1113	3	0.401537122750689
1113	4	0.24482957630735314
1113	5	0.34447376912139305
1113	6	0.06994460727800345
1113	7	0.11857176214518006
1113	8	0.0929372859274693
1113	9	0.4794670632748961
1113	10	0.05750111051307272
1113	11	0.14952592344693727
1113	12	0.14194822227875648
1113	13	0.02637621036099347
1113	14	0.03674847182959695
1113	15	0.17879492699481148
1113	16	0.3530269270721078
1113	17	0.5937089469455756
1113	18	0.5921091007746924
1113	19	0.5662962891477505
1113	20	0.3979107445388763
1113	21	0.40360444382773863
1113	22	0.4611370715735177
1113	23	0.28160280238293467
1113	24	-0.0611433402139383
1113	25	0.41623858419008286
1114	1	-0.07290451397187425
1114	2	-0.3021543756376417
1114	3	-0.2417165327961115
1114	4	0.14795539716367045
1114	5	-0.1087089644395931
1114	6	-0.3711901444950456
1114	7	-0.09556010021363193
1114	8	-0.3894372872272456
1114	9	-0.29158625955147954
1114	10	-0.15127220180634282
1114	11	-0.23628967938994094
1114	12	-0.16226331786658932
1114	13	-0.14560522734148407
1114	14	-0.4357145349123712
1114	15	-0.25873243488243247
1114	16	-0.12546007056382535
1114	17	-0.013926111280497366
1114	18	0.053452240604826784
1114	19	0.07598896054255277
1114	20	-0.016221117819931408
1114	21	-0.032416920326014065
1114	22	-0.04549106956999083
1114	23	-0.06855917993034666
1114	24	-0.24478750447276099
1114	25	0.1338114762310596
1115	1	0.29866115035543217
1115	2	0.16406149445097995
1115	3	0.5551347214087627
1115	4	0.341695471954921
1115	5	0.2040482372065
1115	6	0.5195429122131117
1115	7	0.11431644299624111
1115	8	0.08292580892561574
1115	9	0.16505018325370457
1115	10	0.5158042799312739
1115	11	0.12332304536801075
1115	12	-0.08395448805937633
1115	13	0.20441099450507494
1115	14	0.023858915901278976
1115	15	0.13941998217270424
1115	16	0.4102706809345591
1115	17	0.15391023590117214
1115	18	0.3216932801690575
1115	19	0.8459290250884237
1115	20	0.4841118569252103
1115	21	0.5126712752923837
1115	22	0.3964774088208003
1115	23	0.5313247057995554
1115	24	0.6412668301753968
1115	25	0.4889674620924396
1116	1	0.5636893448442066
1116	2	0.3863546462047118
1116	3	0.506938104747416
1116	4	0.5244986070402149
1116	5	0.27242430558256814
1116	6	0.21427994097503955
1116	7	0.13568396436376418
1116	8	0.045510481053852914
1116	9	0.017055715757166467
1116	10	0.27797756077675473
1116	11	0.3107188676186041
1116	12	0.2476765365177087
1116	13	0.23726909997728995
1116	14	0.27115472751244596
1116	15	0.2809953270675507
1116	16	0.1394971943934351
1116	17	0.22760601914052359
1116	18	0.4184411611493033
1116	19	0.46915135370494454
1116	20	0.42869816043267406
1116	21	0.33573755893957724
1116	22	0.18876027579246113
1116	23	0.5685095126939004
1116	24	0.19506687982607218
1116	25	0.39734423976382516
1201	1	0.2763379625207598
1201	2	0.387548362482228
1201	3	0.1622883411032383
1201	4	0.13747191554425342
1201	5	0.10348957798862557
1201	6	-0.1626240364875997
1201	7	-0.07004109890320365
1201	8	-0.10527975912185639
1201	9	0.29824752208863714
1201	10	0.2872407990902701
1201	11	0.24043285319851293
1201	12	0.33448358421861
1201	13	0.2640322189191764
1201	14	0.2750701169026968
1201	15	0.6046834328785593
1201	16	0.04524135712084387
1201	17	-0.011948963431215986
1201	18	0.0851555764670735
1201	19	0.5328480542731526
1201	20	0.36683978293184083
1201	21	0.4688130495569709
1201	22	0.5939553290322124
1201	23	0.804650466711955
1201	24	0.6057769022679338
1201	25	0.6755040067504012
1202	1	-0.1444394058377334
1202	2	-0.21520927450814398
1202	3	-0.3101650049088853
1202	4	-0.20304276411818023
1202	5	-0.008717720240305482
1202	6	-0.35345964804114516
1202	7	-0.11440696125907834
1202	8	-0.22408399066827656
1202	9	0.17840323348765708
1202	10	0.14154366370456017
1202	11	-0.3408996084679572
1202	12	-0.06656241601911717
1202	13	-0.4129854917666691
1202	14	-0.537719613276785
1202	15	0.19475237380331833
1202	16	-0.12250498782150743
1202	17	-0.19033360624590756
1202	18	-0.3919334524168008
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2210	3	-0.2622926644833541
2210	4	0.03483452435287404
2210	5	-0.04711455349117699
2210	6	-0.7091746406190822
2210	7	-0.2810323942130637
2210	8	-0.1301206062051392
2210	9	-0.1348822687389628
2210	10	-0.6642208093285902
2210	11	-0.7231113848162991
2210	12	-0.7008028910314863
2210	13	-0.6295367040378181
2210	14	-0.6608269858646167
2210	15	-0.3500521783985562
2210	16	-0.4949691144914752
2210	17	-0.40332222574120635
2210	18	-0.1788953524662773
2210	19	-0.4041143986305755
2210	20	-0.6389027141449404
2210	21	-0.766707283040148
2210	22	-0.5840880644977346
2210	23	-0.7057302010806907
2210	24	-0.33061389934307783
2210	25	-0.413647610762915
2211	1	-0.45917967802481385
2211	2	-0.4991847393346731
2211	3	-0.3163467185374067
2211	4	-0.481507398965789
2211	5	-0.49865446549620174
2211	6	-0.3351541084590153
2211	7	-0.6559538272740326
2211	8	-0.6154966799533028
2211	9	-1.0550163563149084
2211	10	-0.5429972601980637
2211	11	-0.5020554451557118
2211	12	-0.8867655979244233
2211	13	-0.41715455444636973
2211	14	-0.8368378804801608
2211	15	-1.2360986900264663
2211	16	-0.9422768873806433
2211	17	-0.8598275682930634
2211	18	-0.6840074412993005
2211	19	-0.8819689974154059
2211	20	-1.0090472773127601
2211	21	-0.7786075554055358
2211	22	-1.235125148092962
2211	23	-0.8050178608334733
2211	24	-0.8927132079540172
2211	25	-0.44626860384902045
2212	1	0.25172353287839755
2212	2	0.06584182569189423
2212	3	0.09668169449100361
2212	4	-0.2703731878315807
2212	5	0.191227724385989
2212	6	0.31684404353914175
2212	7	0.05876088744068397
2212	8	0.011322446865818847
2212	9	-0.10260240390095277
2212	10	0.28225106505026076
2212	11	0.07567613257587169
2212	12	0.3587876476288159
2212	13	-0.01914115643297265
2212	14	-0.24593928958157107
2212	15	0.016038062110283136
2212	16	-1.1596481047518594
2212	17	-0.2595734680389654
2212	18	0.033825776533916496
2212	19	-0.09067770612411152
2212	20	-0.3063445746100584
2212	21	-0.35657013336811616
2212	22	-0.19158160454941253
2212	23	-0.1836272394428491
2212	24	0.16087884563803811
2212	25	0.34606218848177406
2213	1	0.6534329345877978
2213	2	0.30088456073462666
2213	3	0.7035193013286118
2213	4	0.8407379232795336
2213	5	0.4690055021637676
2213	6	0.453596180291278
2213	7	0.571581400261195
2213	8	0.3275617631051375
2213	9	0.47859417729562637
2213	10	0.5771228599220564
2213	11	0.772257329157064
2213	12	0.18784747668864554
2213	13	0.8227391854473751
2213	14	0.8309837873415091
2213	15	0.7938158398880617
2213	16	1.0027450576412988
2213	17	0.924187215721723
2213	18	0.6919454346535758
2213	19	0.665732550286144
2213	20	0.8004930322275499
2213	21	0.8656520888541053
2213	22	0.5520081390403249
2213	23	0.6240650682494575
2213	24	0.8019044866636804
2213	25	0.7221305645501488
2214	1	0.18718820884940257
2214	2	0.46609066264959154
2214	3	0.5351756899470246
2214	4	0.43940718650575405
2214	5	0.15763473509679216
2214	6	0.37142588926022313
2214	7	0.12275526156164318
2214	8	0.2667589934416057
2214	9	0.025494534322561435
2214	10	0.001876727713899129
2214	11	0.4472546244075275
2214	12	-0.10058701427748673
2214	13	0.11625860554907064
2214	14	-0.1181128263120712
2214	15	0.4622135462604504
2214	16	0.06581981691859085
2214	17	0.5149586102905843
2214	18	0.14179937817840482
2214	19	0.28555295491667465
2214	20	0.18943601610724414
2214	21	0.16631204774191133
2214	22	0.48000770628040357
2214	23	0.3521836444692994
2214	24	0.4582390100868068
2214	25	0.48308480018792466
2215	1	0.029465992439035915
2215	2	0.30395724562549375
2215	3	0.23357510411168647
2215	4	0.0047557236609705456
2215	5	-0.01496002725630774
2215	6	0.08886495192368216
2215	7	-0.19973314378061957
2215	8	-0.23067797695278358
2215	9	-0.014624725014186879
2215	10	-0.17035188755269104
2215	11	0.07087285504531593
2215	12	-0.2926921856691962
2215	13	-0.0870227697691277
2215	14	0.21461026187706977
2215	15	-0.06254497101820533
2215	16	-0.2843214112433472
2215	17	-0.10597454171276155
2215	18	0.03619209708205773
2215	19	-0.511706172607127
2215	20	-0.4013858969241113
2215	21	-0.0874453187887525
2215	22	-0.27789932723077726
2215	23	-0.561178980630956
2215	24	-0.5019381353607599
2215	25	-0.2740550679991216
2216	1	0.08917590647534723
2216	2	0.054977856996597296
2216	3	0.06300854033592174
2216	4	0.015127790440480737
2216	5	0.5775422131101138
2216	6	0.2633480081153934
2216	7	0.166525278337609
2216	8	-0.14653977123735729
2216	9	0.22990712137965374
2216	10	0.0684213873651558
2216	11	0.15326855354178548
2216	12	0.3372656622514114
2216	13	0.37221915661409355
2216	14	0.40656817617890795
2216	15	0.4606884585679083
2216	16	0.523803650989052
2216	17	0.5589828083486381
2216	18	0.3147443225127091
2216	19	0.2339541546523236
2216	20	0.21373574667508421
2216	21	0.3527286000270067
2216	22	0.4744157180744111
2216	23	0.32615548118324256
2216	24	0.3230145680147203
2216	25	0.7170332522239207
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	133.0
3	17.0
4	10.0
5	13.0
6	16.0
7	29.0
8	15.0
9	31.0
10	78.0
11	129.0
12	173.0
13	276.0
14	518.0
15	812.0
16	1146.0
17	1321.0
18	1343.0
19	1298.0
20	1196.0
21	1040.0
22	907.0
23	847.0
24	749.0
25	703.0
26	657.0
27	619.0
28	552.0
29	514.0
30	580.0
31	826.0
32	1012.0
33	1346.0
34	1938.0
35	3105.0
36	6139.0
37	16110.0
38	90326.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.82470148707054	23.13530144311772	10.802871584499304	23.237125485312433
2	11.498268067868256	28.60623495567428	30.74840016438678	29.14709681207069
3	22.95169801903895	34.75085689960146	24.91284211762461	17.38460296373498
4	14.680454438703617	26.599195631752	24.647721935180837	34.07262799436355
5	10.728284325958985	42.26720885509182	32.755912447334815	14.24859437161438
6	30.740221721681326	32.17165454852271	17.053933688929323	20.034190040866637
7	18.267198872974877	38.86695820615168	21.38632895045785	21.47951397041559
8	26.815580985915492	35.19879694835681	20.957306338028168	17.028315727699532
9	25.551397754787587	22.143957737178077	18.625724557927946	33.67891995010639
10	18.3957384049836	35.246208369103435	27.44355661214936	18.9144966137636
11	23.95099765258216	30.821596244131456	15.678550469483568	29.548855633802816
12	24.074535984153766	37.28486538038295	24.301958770449712	14.338639865013572
13	29.6078805584509	24.337536885065404	20.429555030315484	25.62502752616821
14	21.052747430131117	33.86137015650336	22.648597486261014	22.437284927104503
15	36.10964371661677	22.25157011210894	20.202353700768917	21.43643247050537
16	16.042580664094025	30.868046894487406	32.78138893372265	20.30798350769592
17	26.050210555661522	34.38293251947823	23.851481226064884	15.71537569879536
18	25.383723660268824	25.469566238187475	28.74699184128661	20.39971826025709
19	22.532111706927033	35.73257238429882	28.43729139310891	13.298024515665233
20	24.245714956562573	27.407401972293965	25.069705329889647	23.277177741253816
21	17.885445099938362	32.7037656658155	28.9791905139268	20.431598720319332
22	22.522575391905868	31.637825426750098	18.600949230125952	27.23864995121808
23	15.95988202321384	30.920483059179148	27.412727993074	25.70690692453301
24	21.68422751819676	38.85888706269077	20.996712843390466	18.460172575722
25	22.452877496239047	26.485944367422793	19.30031562490782	31.760862511430343
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3636.0
1	3636.0
2	2613.0
3	1590.0
4	1590.0
5	1590.0
6	1479.5
7	1369.0
8	1369.0
9	1369.0
10	1379.5
11	1390.0
12	1390.0
13	1390.0
14	1278.5
15	1167.0
16	1167.0
17	1167.0
18	1245.0
19	1323.0
20	1323.0
21	1323.0
22	1720.0
23	2117.0
24	2117.0
25	2117.0
26	2580.0
27	3043.0
28	3043.0
29	3043.0
30	3364.5
31	3686.0
32	3686.0
33	3686.0
34	3949.0
35	4212.0
36	4212.0
37	4212.0
38	4322.5
39	4433.0
40	4433.0
41	4433.0
42	6404.5
43	8376.0
44	8376.0
45	8376.0
46	29124.0
47	49872.0
48	49872.0
49	49872.0
50	41443.5
51	33015.0
52	33015.0
53	33015.0
54	21818.5
55	10622.0
56	10622.0
57	10622.0
58	6705.5
59	2789.0
60	2789.0
61	2789.0
62	2346.5
63	1904.0
64	1904.0
65	1904.0
66	1474.5
67	1045.0
68	1045.0
69	1045.0
70	797.5
71	550.0
72	550.0
73	550.0
74	394.5
75	239.0
76	239.0
77	239.0
78	170.0
79	101.0
80	101.0
81	101.0
82	65.5
83	30.0
84	30.0
85	30.0
86	20.5
87	11.0
88	11.0
89	11.0
90	7.0
91	3.0
92	3.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01025460724854238
2	0.1904427060443585
3	0.20289472913187426
4	0.19630248161495414
5	0.20948697664879434
6	0.16627113181565148
7	0.17286337933257157
8	0.14942427705018899
9	0.17139843543992264
10	0.17359585127889604
11	0.14942427705018899
12	0.15674899651343355
13	0.2124168644340922
14	0.1706659634935982
15	0.16700360376197593
16	0.15967888429873137
17	0.15967888429873137
18	0.16700360376197593
19	0.1486918051038645
20	0.17286337933257157
21	0.17579326711786938
22	0.1486918051038645
23	0.165538659869327
24	0.17286337933257157
25	0.6738741906184993
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	136524.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.339134511148227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.50598369659478	21.41308487884914
2	8.98999826559519	4.555975506138115
3	2.6189512632248366	1.990858750109871
4	1.1418164999710931	1.15730567519264
5	0.48852402150661967	0.6189387946441651
6	0.3353182632826502	0.5098004746418212
7	0.2803954442966989	0.49734845155430546
8	0.18789385442562295	0.380885412088717
9	0.14742440885702723	0.33620462336292517
>10	0.9394692721281147	4.68342562479857
>50	0.11273631265537377	2.0274823474260937
>100	0.14742440885702723	8.326008613870089
>500	0.026016072151240098	5.083355307491723
>1k	0.07226686708677806	36.12478392077583
>5k	0.005781349366942245	12.294541619055991
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCAC	8442	6.183528170871055	No Hit
GTACATGGGAAGCAGTGGTATCAAC	8343	6.111013448184935	No Hit
GAGTACATGGGAAGCAGTGGTATCA	4214	3.0866367818112566	No Hit
CATGTACTCTGCGTTGATACCACTG	4172	3.0558729600656296	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	3404	2.4933345052884475	No Hit
GCGTTGATACCACTGCTTCCCATGT	3073	2.2508862910550524	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	3003	2.1996132548123404	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2712	1.986463918431924	No Hit
GTATCAACGCAGAGTACATGGGAAG	2511	1.8392370572207086	No Hit
TATCAACGCAGAGTACATGGGAAGC	2503	1.8333772816501128	No Hit
GGTATCAACGCAGAGTACATGGGAA	2469	1.8084732354750814	No Hit
GTGGTATCAACGCAGAGTACATGGG	1870	1.3697225396267323	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1768	1.2950104011016377	No Hit
GTACTCTGCGTTGATACCACTGCTT	1673	1.2254255662008144	No Hit
ATACCACTGCTTCCCATGTACTCTG	1541	1.1287392692859863	No Hit
GATACCACTGCTTCCCATGTACTCT	1478	1.0825935366675457	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	1410	1.0327854443174826	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	1365	0.9998242067328821	No Hit
CATGGGAAGCAGTGGTATCAACGCA	1215	0.8899534147842139	No Hit
CAGTGGTATCAACGCAGAGTACATG	1185	0.8679792563944801	No Hit
GCAGTGGTATCAACGCAGAGTACAT	1180	0.8643168966628579	No Hit
ACATGGGAAGCAGTGGTATCAACGC	1149	0.8416102663267996	No Hit
GTTGATACCACTGCTTCCCATGTAC	1135	0.8313556590782574	No Hit
CCATGTACTCTGCGTTGATACCACT	1116	0.8174386920980926	No Hit
TACCACTGCTTCCCATGTACTCTGC	1095	0.802056781225279	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	1057	0.7742228472649497	No Hit
CCACTGCTTCCCATGTACTCTGCGT	1021	0.7478538571972693	No Hit
CTTCCCATGTACTCTGCGTTGATAC	941	0.6892561014913129	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	938	0.6870586856523395	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	865	0.6335882335706543	No Hit
GTATCAACGCAGAGTACTTTTTTTT	813	0.5954996923617826	No Hit
TTCCCATGTACTCTGCGTTGATACC	795	0.5823151973279423	No Hit
TGATACCACTGCTTCCCATGTACTC	767	0.5618059828308576	No Hit
ATCAACGCAGAGTACATGGGAAGCA	653	0.47830418094986965	No Hit
GGTATCAACGCAGAGTACTTTTTTT	640	0.4687820456476517	No Hit
GTACATGGTAAGCAGTGGTATCAAC	528	0.38674518765931265	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	494	0.36184114148428115	No Hit
ACCATGTACTCTGCGTTGATACCAC	479	0.3508540622894143	No Hit
TATCAACGCAGAGTACTTTTTTTTT	455	0.3332747355776274	No Hit
TCAACGCAGAGTACATGGGAAGCAG	401	0.29372125047610675	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	393	0.2878614749055111	No Hit
CTCTGCGTTGATACCACTGCTTCCC	377	0.27614192376431984	No Hit
CTGCGTTGATACCACTGCTTCCCAT	375	0.2746769798716709	No Hit
AAAAAGTACTCTGCGTTGATACCAC	363	0.26588731651577746	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	330	0.24171574228707043	No Hit
TCCCATGTACTCTGCGTTGATACCA	309	0.22633383141425684	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	307	0.22486888752160794	No Hit
ATGTACTCTGCGTTGATACCACTGC	306	0.22413641557528347	No Hit
TCCATGTACTCTGCGTTGATACCAC	295	0.21607922416571448	No Hit
TTGATACCACTGCTTCCCATGTACT	293	0.21461428027306556	No Hit
AAAGTACTCTGCGTTGATACCACTG	291	0.21314933638041664	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	266	0.19483753772230522	No Hit
CCCCATGTACTCTGCGTTGATACCA	259	0.18971023409803403	No Hit
GAGTACATGGTAAGCAGTGGTATCA	252	0.18458293047376287	No Hit
AGTGGTATCAACGCAGAGTACATGG	245	0.17945562684949168	No Hit
AACGCAGAGTACATGGGAAGCAGTG	240	0.17579326711786938	No Hit
ACTGCTTCCCATGTACTCTGCGTTG	222	0.16260877208402919	No Hit
GTACATGGAAAGCAGTGGTATCAAC	210	0.15381910872813573	No Hit
CAACGCAGAGTACATGGGAAGCAGT	200	0.14649438926489117	No Hit
CGCAGAGTACATGGGAAGCAGTGGT	199	0.1457619173185667	No Hit
GTACATGGGTAAGCAGTGGTATCAA	199	0.1457619173185667	No Hit
GCTTACCATGTACTCTGCGTTGATA	199	0.1457619173185667	No Hit
TGTACTCTGCGTTGATACCACTGCT	196	0.14356450147959332	No Hit
ACTCTGCGTTGATACCACTGCTTAC	196	0.14356450147959332	No Hit
CGTTGATACCACTGCTTCCCATGTA	178	0.13038000644575312	No Hit
CTGCTTACCATGTACTCTGCGTTGA	170	0.12452023087515748	No Hit
GCGTTGATACCACTGCTTACCATGT	162	0.11866045530456183	No Hit
ACGCAGAGTACATGGTAAGCAGTGG	153	0.11206820778764173	No Hit
AAAAACAAAAAAAAAAAAAAAAAAA	148	0.10840584805601944	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	144	0.10547596027072163	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	142	0.10401101637807272	No Hit
GAGTACATGGAAAGCAGTGGTATCA	137	0.10034865664645044	No Hit
GTGGTATCAACGCAGAGTACTTTTT	137	0.10034865664645044	No Hit
GCTTTCCATGTACTCTGCGTTGATA	137	0.10034865664645044	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	7.324719463244558E-4	0.0
6	0.0	0.0	0.0	7.324719463244558E-4	0.0
7	0.0	0.0	0.0	7.324719463244558E-4	0.0
8	0.0	0.0	0.0	7.324719463244558E-4	0.0
9	0.0	0.0	0.0	7.324719463244558E-4	0.0
10	0.0	0.0	0.0	7.324719463244558E-4	0.0
11	0.0	0.0	0.0	7.324719463244558E-4	0.0
12	0.0	0.0	0.0	7.324719463244558E-4	0.0
13	0.0	0.0	0.0	7.324719463244558E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACAAA	85	5.4569682E-11	16.761145	1
AAAAAGT	75	0.0026257953	10.131181	1
AAAAGTA	85	0.0073753605	8.939278	2
GAAAAAA	235	5.820766E-10	8.487558	1
CATGGGT	105	0.0044670114	8.141128	4
AAGTACT	110	0.006784621	7.7710767	2
CATGGTA	150	2.2707593E-4	7.5983853	4
CCATGTA	2705	0.0	7.0225377	2
ACATGGT	170	9.527974E-4	6.704458	3
GAAGCAG	2480	0.0	6.587311	9
AGTGGTA	2710	0.0	6.5189104	14
CAGTGGT	2715	0.0	6.5069046	13
GGAAGCA	2530	0.0	6.4571266	8
CCCATGT	2750	0.0	6.4240904	1
TGTACTC	2975	0.0	6.3851986	5
ATGTACT	2985	0.0	6.363807	4
TACTCTG	3110	0.0	6.3523483	7
TGATACC	3130	0.0	6.311758	17
TGCGTTG	3160	0.0	6.2818937	12
CTGCGTT	3165	0.0	6.2719693	11
>>END_MODULE